CD84/SLAMF5 Antibody (6) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89267

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6 expressed in HEK293

Format

Azide and BSA Free
View all CD84/SLAMF5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD84/SLAMF5 extracellular domain (Met 1-Gly 225; Accession#: NP_003865.1).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD84/SLAMF5 Antibody (6) - Azide and BSA Free

Western Blot: CD84/SLAMF5 Antibody (6) [NBP2-89267]

Western Blot: CD84/SLAMF5 Antibody (6) [NBP2-89267]

Western Blot: CD84/SLAMF5 Antibody (6) [NBP2-89267] - Lane A: RAW264.7 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:39 kDa Observed band size:39 kDa
Immunoprecipitation: CD84/SLAMF5 Antibody (6) [NBP2-89267]

Immunoprecipitation: CD84/SLAMF5 Antibody (6) [NBP2-89267]

Immunoprecipitation: CD84/SLAMF5 Antibody (6) [NBP2-89267] - Lane A:0.5 mg Raw264.7 Whole Cell Lysate 0.5 uL anti-CD84 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD84 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 62 kDaObserved band size: 62 kDa

Applications for CD84/SLAMF5 Antibody (6) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD84/SLAMF5

CD84 is a 64-82 kD glycoprotein. It is a member of the SLAM (CD150) family, a CD2 subset of the Ig superfamily, also known as SLAMF5 or Ly9b. CD84 is expressed on B cells, monocytes, thymocytes, subset of T cells, and platelets. CD84 functions as a homophilic adhesion molecule and enhances T cell activation and cytokine production. CD84 is expressed on mature B cells and B cell lines but not on plasma cell lines. Immunohistochemical studies demonstrate that it strongly stains tissue macrophages. It is also expressed on platelets and at low levels on blood T cells. Cellular expression does not significantly increase after activation.

Long Name

SLAM Family Member 5

Alternate Names

CD84, Ly-9B, SLAMF5

Gene Symbol

CD84

Additional CD84/SLAMF5 Products

Product Documents for CD84/SLAMF5 Antibody (6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD84/SLAMF5 Antibody (6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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