CD9 Antibody (10N2L6)
Novus Biologicals | Catalog # NBP3-15593
Recombinant Monoclonal Antibody
![Western Blot: CD9 Antibody (10N2L6) [NBP3-15593]](https://resources.rndsystems.com/images/products/CD9-Antibody-10N2L6-Western-Blot-NBP3-15593-img0002.jpg "Western Blot: CD9 Antibody (10N2L6) [NBP3-15593]")
Loading...
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10N2L6 expressed in HEK293
Loading...
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 129-228 of human CD9 (P21926). YNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHIIGAVGIGIAVVMIFGMIFSMILCCAIRRNREMV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CD9 Antibody (10N2L6)
Western Blot: CD9 Antibody (10N2L6) [NBP3-15593]
Western Blot: CD9 Antibody (10N2L6) [NBP3-15593] - Analysis of extracts of various cell lines, using CD9 antibody (NBP3-15593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.![Immunohistochemistry-Paraffin: CD9 Antibody (10N2L6) [NBP3-15593]](https://resources.rndsystems.com/images/products/CD9-Antibody-10N2L6-Immunohistochemistry-Paraffin-NBP3-15593-img0001.jpg "Immunohistochemistry-Paraffin: CD9 Antibody (10N2L6) [NBP3-15593]")
Immunohistochemistry-Paraffin: CD9 Antibody (10N2L6) [NBP3-15593]
Immunohistochemistry-Paraffin: CD9 Antibody (10N2L6) [NBP3-15593] - Human lung cancer using CD9 Rabbit mAb (NBP3-15593) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunoprecipitation: CD9 Antibody (10N2L6) [NBP3-15593]](https://resources.rndsystems.com/images/products/CD9-Antibody-10N2L6-Immunoprecipitation-NBP3-15593-img0003.jpg "Immunoprecipitation: CD9 Antibody (10N2L6) [NBP3-15593]")
Immunoprecipitation: CD9 Antibody (10N2L6) [NBP3-15593]
Immunoprecipitation: CD9 Antibody (10N2L6) [NBP3-15593] - Analysis of 300ug extracts of THP-1 cells using 3ug CD9 antibody (NBP3-15593). Western blot was performed from the immunoprecipitate using CD9 antibody (NBP3-15593) at a dilition of 1:1000.Applications for CD9 Antibody (10N2L6)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CD9
Alternate Names
CD9, DRAP-27, MIC3, p24, TSPAN29
Gene Symbol
CD9
Additional CD9 Products
Product Documents for CD9 Antibody (10N2L6)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD9 Antibody (10N2L6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for CD9 Antibody (10N2L6)
There are currently no reviews for this product. Be the first to review CD9 Antibody (10N2L6) and earn rewards!
Have you used CD9 Antibody (10N2L6)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...