CD9 Antibody (EM-04) - BSA Free

Novus Biologicals | Catalog # NBP1-44876

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # EM-04

Format

BSA Free
View all CD9 Primary Antibodies »

Product Specifications

Immunogen

Permeabilized murine bone marrow-derived mast cells (BMMC).

Specificity

The rat monoclonal antibody EM-04 recognizes CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD9 Antibody (EM-04) - BSA Free

Western Blot: CD9 Antibody (EM-04)BSA Free [NBP1-44876]

Western Blot: CD9 Antibody (EM-04)BSA Free [NBP1-44876]

Western Blot: CD9 Antibody (EM-04) [NBP1-44876] - Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody.
Flow Cytometry: CD9 Antibody (EM-04) - BSA Free [NBP1-44876]

Flow Cytometry: CD9 Antibody (EM-04) - BSA Free [NBP1-44876]

Flow Cytometry: CD9 Antibody (EM-04) [NBP1-44876] - Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) APC.
Flow Cytometry: CD9 Antibody (EM-04) - BSA Free [NBP1-44876]

Flow Cytometry: CD9 Antibody (EM-04) - BSA Free [NBP1-44876]

Flow Cytometry: CD9 Antibody (EM-04) [NBP1-44876] - Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) PE
Flow Cytometry: CD9 Antibody (EM-04) - BSA Free [NBP1-44876]

Flow Cytometry: CD9 Antibody (EM-04) - BSA Free [NBP1-44876]

Flow Cytometry: CD9 Antibody (EM-04) [NBP1-44876] - Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) purified, DAR-APC.

Applications for CD9 Antibody (EM-04) - BSA Free

Application
Recommended Usage

Flow Cytometry

4 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:50

Western Blot

1 ug/ml
Application Notes
Western Blotting: non-reducing conditions. This antibody is CyTOF ready.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD9

This antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily. This family is characterized by the presence of four hydrophobic domains spanning the cell membrane and short N- and C-terminal cytoplasmic domains. CD9 is expressed by several cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts and neuroblasts. On T cells, CD9 functions as a co-stimulatory molecule on na've T cells. Furthermore, CD9 is expressed in oocytes, and CD9-deficiency results in sterility caused by defective gamete fusion. In mouse macrophages, CD9 functionally associates with FcgammaRs to modify signals for phagocytosis and inflammatory responses. In mouse B cells, it was discovered that CD9 is a marker for marginal zone B cells, B1 cells, and plasma cells. In dendritic cells, recently it was demonstrated that CD9 facilitates the association of heterologous MHC II molecules. The level of CD9 expression is subject to donor variability.

Alternate Names

CD9, DRAP-27, MIC3, p24, TSPAN29

Gene Symbol

CD9

Additional CD9 Products

Product Documents for CD9 Antibody (EM-04) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD9 Antibody (EM-04) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD9 Antibody (EM-04) - BSA Free

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Protocols

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FAQs

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