Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # UM7F8
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Product Specifications
Immunogen
Molt 13 T cell line
Localization
Cell surface, cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34506)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD98 Antibody (UM7F8)
Immunocytochemistry/Immunofluorescence: CD98 Antibody (UM7F8) [NBP2-34291] -
Immunocytochemistry/Immunofluorescence: CD98 Antibody (UM7F8) [NBP2-34291] - Analysis of CD89 antibody at 1:200 dilution. Fixation in 4% PAF and experiment done without permeabilization. Primary antibody (1/200) in blocking buffer, overnight @4°C. Secondary AB anti-Mouse IgG - AF594 1/600, 1h, R.T. Confocal microscopy. Image from verified customer review.Applications for CD98 Antibody (UM7F8)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Application Notes
Comitogenic with soluble anti-CD2 and immobilized anti-CD3 mAbs.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Reviewed Applications
Read 1 review rated 5 using NBP2-34291 in the following applications:
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD98
Additional CD98 Products
Product Documents for CD98 Antibody (UM7F8)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD98 Antibody (UM7F8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for CD98 Antibody (UM7F8) (1)
5 out of 5
1 Customer Rating
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Application: ImmunofluorescenceSample Tested: hela cell, HT-29 colon cancer cell line and HCT-116 human colorectal carcinoma cell lineSpecies: HumanVerified Customer | Posted 04/17/2023IF images using anti-CD98 (NBP2-34291) (1:200)fixation in 4% PAF no permeabilisation primary AB 1/200 in blocking buffer, o.night @4°C secondary AB anti-Mouse IgG -AF594 1/600, 1h, R.T. Confocal microscopy
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
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- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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