CDC42 Antibody (JJ086-04)

Novus Biologicals | Catalog # NBP2-67895

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JJ086-04 expressed in HEK293
View all CDC42 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human CDC42 aa 30-191 / 191. (SwissProt: P60953 Human; SwissProt: P60766 Mouse; SwissProt: Q8CFN2 Rat)

Localization

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC42 Antibody (JJ086-04)

Western Blot: CDC42 Antibody (JJ086-04) [NBP2-67895]

Western Blot: CDC42 Antibody (JJ086-04) [NBP2-67895]

Western Blot: CDC42 Antibody (JJ086-04) [NBP2-67895] - Western blot analysis of CDC42 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: Jurkat cell lysate
Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895]

Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895]

Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.
Flow Cytometry: CDC42 Antibody (JJ086-04) [NBP2-67895]

Flow Cytometry: CDC42 Antibody (JJ086-04) [NBP2-67895]

Flow Cytometry: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895]

Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895]

Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-CDC42 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895]

Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895]

Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of paraffin-embedded human pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.

Applications for CDC42 Antibody (JJ086-04)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC42

CDC42 is a small GTPase of the Rho subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc42, and is able to complement the yeast cdc42 1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott Aldrich syndrome protein (NWASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in at least two transcript variants.

Long Name

Cell Division Cycle 42

Alternate Names

G25K

Gene Symbol

CDC42

Additional CDC42 Products

Product Documents for CDC42 Antibody (JJ086-04)

Certificate of Analysis

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Product Specific Notices for CDC42 Antibody (JJ086-04)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

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Protocols

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