Cdk5 Antibody (OTI2H6)

Novus Biologicals | Catalog # NBP1-47692

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2H6
View all CDK5 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CDK5 (NP_004926) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens cyclin-dependent kinase 5 (CDK5), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Cdk5 Antibody (OTI2H6) (NBP1-47692) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Cdk5 Antibody (OTI2H6)

Western Blot: Cdk5 Antibody (2H6) [NBP1-47692] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk5 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Immunocytochemistry/Immunofluorescence: Cdk5 Antibody (2H6) [NBP1-47692] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk5.
Flow Cytometry: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Flow Cytometry: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Flow Cytometry: Cdk5 Antibody (2H6) [NBP1-47692] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cdk5 antibody, and then analyzed by flow cytometry.
Western Blot: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Western Blot: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Western Blot: Cdk5 Antibody (2H6) [NBP1-47692] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cdk5.
Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Immunocytochemistry/Immunofluorescence: Cdk5 Antibody (2H6) [NBP1-47692] - Immunofluorescent staining of HT29 cells using anti-Cdk5 mouse monoclonal antibody.

Applications for Cdk5 Antibody (OTI2H6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Western Blot

1:1000-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDK5

Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase

Long Name

Cyclin-dependent Kinase 5

Alternate Names

CDKN5, LIS7, PSSALRE

Entrez Gene IDs

1020 (Human)

Gene Symbol

CDK5

Additional CDK5 Products

Product Documents for Cdk5 Antibody (OTI2H6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cdk5 Antibody (OTI2H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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