CEACAM3/CD66d Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92411

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human CEACAM3/CD66d (NP_001806.2). MGPPSASPHRECIPWQGLLLTASLLNFWNPPTTAKLTIESMPLSVAEGKEVLLLVHNLPQHLFGYSWYKGERVDGNSLIVGYVIGTQQATPGAAYSGRETIYTNASLLIQNVTQNDIGFYTLQVIKSDLVNEEAT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CEACAM3/CD66d Antibody - BSA Free

CEACAM3/CD66d Antibody - BSA Free

Western Blot: CEACAM3/CD66d Antibody - BSA Free [NBP2-92411] -

Western Blot: CEACAM3/CD66d Antibody - BSA Free [NBP2-92411] - Western blot analysis of lysates from A549 cells, using CEACAM3 at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Applications for CEACAM3/CD66d Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CEACAM-3/CD66d

CEACAM-3 (carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31 - 34 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms. Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein. Its extracellular domain (aa 35 - 155) shows one V-type Ig-like domain (aa 35 - 142). There are two alternate splice forms of CEACAM-3. One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137 - 252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids. No definitive rodent CEACAM-3 has been reported.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 3

Alternate Names

CD66d, CGM1

Gene Symbol

CEACAM3

Additional CEACAM-3/CD66d Products

Product Documents for CEACAM3/CD66d Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CEACAM3/CD66d Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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