CEACAM-3 (carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31 - 34 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms. Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein. Its extracellular domain (aa 35 - 155) shows one V-type Ig-like domain (aa 35 - 142). There are two alternate splice forms of CEACAM-3. One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137 - 252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids. No definitive rodent CEACAM-3 has been reported.
CEACAM3/CD66d Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-92411
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human CEACAM3/CD66d (NP_001806.2). MGPPSASPHRECIPWQGLLLTASLLNFWNPPTTAKLTIESMPLSVAEGKEVLLLVHNLPQHLFGYSWYKGERVDGNSLIVGYVIGTQQATPGAAYSGRETIYTNASLLIQNVTQNDIGFYTLQVIKSDLVNEEAT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CEACAM3/CD66d Antibody - BSA Free
Western Blot: CEACAM3/CD66d Antibody - BSA Free [NBP2-92411] -
Western Blot: CEACAM3/CD66d Antibody - BSA Free [NBP2-92411] - Western blot analysis of lysates from A549 cells, using CEACAM3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Applications for CEACAM3/CD66d Antibody - BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL.
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CEACAM-3/CD66d
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 3
Alternate Names
CD66d, CGM1
Gene Symbol
CEACAM3
Additional CEACAM-3/CD66d Products
Product Documents for CEACAM3/CD66d Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CEACAM3/CD66d Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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