CEACAM5/CD66e Antibody (308/3-3) - BSA Free

Novus Biologicals | Catalog # NBP2-54624

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 308/3-3

Format

BSA Free
View all CEACAM-5/CD66e Primary Antibodies »

Product Specifications

Immunogen

Recombinant soluble human CEACAM3-Fc produced in HEK293 cells

Epitope

Reacts to an epitope shared by CEACAM 3 and 5

Specificity

Clone 308/3-3 reacts with human CEACAM3 and CEACAM5, but not human CEACAM1/4/6/7/8, rat CEACAM1, or mouse CEACAM1 & CEACAM2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CEACAM5/CD66e Antibody (308/3-3) - BSA Free

Western Blot: CEACAM5/CD66e Antibody (308/3-3)BSA Free [NBP2-54624]

Western Blot: CEACAM5/CD66e Antibody (308/3-3)BSA Free [NBP2-54624]

Western Blot: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Detection using 10 ug/ml Anti-CEACAM3/5 [308/3-3] followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.
Flow Cytometry: CEACAM5/CD66e Antibody (308/3-3) - BSA Free [NBP2-54624]

Flow Cytometry: CEACAM5/CD66e Antibody (308/3-3) - BSA Free [NBP2-54624]

Flow Cytometry: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM3/5 [308/3-3] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).
Sandwich ELISA: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Solid phase was coated with 3 ug/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM3/5 [308/3-3] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
Immunoprecipitation: CEACAM5/CD66e Antibody (308/3-3) - BSA Free [NBP2-54624]

Immunoprecipitation: CEACAM5/CD66e Antibody (308/3-3) - BSA Free [NBP2-54624]

Immunoprecipitation: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Immunoprecipitation utilizing 10 ug/ml Anti-CEACAM3/5 [308/3-3]. Detection of the sample using 5 ug/ml pAb Anti-CEA (Dako) followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.

Applications for CEACAM5/CD66e Antibody (308/3-3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunoprecipitation

1:10 - 1:500

Sandwich ELISA

1:100 - 1:2000

Western Blot

1:100 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CEACAM-5/CD66e

The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Alternate Names

CD66e, CEA, CEACAM5

Gene Symbol

CEACAM5

Additional CEACAM-5/CD66e Products

Product Documents for CEACAM5/CD66e Antibody (308/3-3) - BSA Free

Certificate of Analysis

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Product Specific Notices for CEACAM5/CD66e Antibody (308/3-3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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