CEACAM5/CD66e Antibody (CB30) - BSA Free
Novus Biologicals | Catalog # NB110-58734
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CB30) - BSA Free [NB110-58734]](https://resources.rndsystems.com/images/products/CEACAM-5-CD66e-Antibody-CB30-Immunohistochemistry-Paraffin-NB110-58734-img0002.jpg "Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CB30) - BSA Free [NB110-58734]")
Key Product Details
Species Reactivity
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation, CyTOF-ready
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CB30
Format
BSA Free
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Product Specifications
Immunogen
Human carcinoembryonic antigen (CEA; CEACAM5)
Specificity
The antibody CB30 recognizes CD66e (CEA; 180-200 kDa), a cell surface bound carcinoembryonic antigen mainly expressed on epithelial cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CEACAM5/CD66e Antibody (CB30) - BSA Free
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CB30) - BSA Free [NB110-58734]
Immunohistochemistry-Paraffin: CEACAM-5/CD66e Antibody (CB30) [NB110-58734] - Human colorectal carcinoma![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CB30) - BSA Free [NB110-58734]](https://resources.rndsystems.com/images/products/CEACAM-5-CD66e-Antibody-CB30-Immunohistochemistry-Paraffin-NB110-58734-img0001.jpg "Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CB30) - BSA Free [NB110-58734]")
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CB30) - BSA Free [NB110-58734]
Immunohistochemistry-Paraffin: CEACAM-5/CD66e Antibody (CB30) [NB110-58734] - Human colorectal carcinomaApplications for CEACAM5/CD66e Antibody (CB30) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:200
Immunohistochemistry-Paraffin
10 ug/ml
Immunoprecipitation
1:50
Application Notes
For paraffin sections use standard ABC technique with pretreatment in 0.1% pepsin in 0.1M HCl for 30 minutes at RT. This antibody is CyTOF ready.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CEACAM-5/CD66e
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 5
Alternate Names
CD66e, CEA, CEACAM5
Entrez Gene IDs
1048 (Human)
Gene Symbol
CEACAM5
Additional CEACAM-5/CD66e Products
Product Documents for CEACAM5/CD66e Antibody (CB30) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CEACAM5/CD66e Antibody (CB30) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for CEACAM5/CD66e Antibody (CB30) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
