CellXVivo Human Th1 Cell Differentiation Kit

Kit Summary

For the differentiation of Th1 cells from a preparation of naïve CD4⁺ T cells.

Why Differentiate Th1 Cells In Vitro?

T helper type 1 (Th1) cells are a subset of CD4⁺ effector T cells that promote cell-mediated immune responses against intracellular viral and bacterial pathogens. Differentiation into the Th1 lineage is promoted by cytokines such as IL-12 and IFN-gamma. Th1 cells are characterized by the secretion of IFN-gamma, IL-10, and TNF-alpha. Although Th1 cells are critical for the clearance of intracellular pathogens, exaggerated Th1 responses are associated with autoimmune diseases including rheumatoid arthritis, multiple sclerosis, inflammatory bowel disease, and type 1 diabetes. Th1 polarized cells are present in low abundance in normal human peripheral blood. In vitro differentiation of Th1 cells from the larger naïve CD4⁺ T cell population provides increased numbers of Th1 cells to facilitate downstream research.

This kit contains the following optimized reagents to drive efficient differentiation of naïve CD4⁺ T cells into Th1 polarized cells.

• Mouse Anti-Human CD3 Antibody
• Human Th1 Differentiation Supplements
• Reconstitution Buffers
• Wash Buffer (20X)

Provides sufficient reagents for the differentiation of one 24-well plate.

Stability and Storage

Store the unopened kit at 2 to 8 °C. After opening the kit, Mouse Anti-Human CD3 Antibody, Human Th1 Reagent 1, and Human Th1 Reagent 2 may be stored at 2-8 °C under sterile conditions for up to 30 days or at -20 °C to -70 °C in a manual defrost freezer for up to 3 months. Reconstitution Buffer 1, Reconstitution Buffer 2, and 20X Wash Buffer may be stored under sterile conditions for up to 12 months at 2 to 8 °C. Do not use beyond the expiration date of the kit.