• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)
  • Sensitivity
    10 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-4
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC01-1, Quantikine Immunoassay Control Group 1 - Please Inquire
Product Summary
The Quantikine Human IL-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-4 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human IL-4 accurately. Results obtained using natural human IL-4 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-4.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean1705101110132411865
Standard Deviation6.717.558.59.832.152.8
CV%3.93.45.37.47.86.1

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean15953810921695031032
Standard Deviation11.427.867.216.849.383
CV%7.25.26.29.99.88

Recovery

The recovery of human IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 98 90-109
Citrate Plasma (n=10) 94 85-109
EDTA Plasma (n=10) 100 90-123
Heparin Plasma (n=10) 95 84-121
Serum (n=10) 98 82-111
Linearity
To assess the linearity of the assay, the following biological samples, spiked with high concentrations of IL-4, were diluted with the appropriate Calibrator Diluent and then assayed.
Human IL-4 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-4
IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.
    • Long Name
      Interleukin 4
    • Entrez Gene IDs
      3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit);
    • Alternate Names
      B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 3 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    38 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Profiling of cytokines, chemokines and other soluble proteins as a potential biomarker in colorectal cancer and polyps
      Authors: NA Johdi, L Mazlan, I Sagap, R Jamal
      Cytokine, 2017;99(0):35-42.
      Species: Human
      Sample Type: Serum
    2. The Association between Serum Cytokines and Damage to Large and Small Nerve Fibers in Diabetic Peripheral Neuropathy.
      Authors: Magrinelli F, Briani C, Romano M, Ruggero S, Toffanin E, Triolo G, Peter G, Praitano M, Lauriola M, Zanette G, Tamburin S
      J Diabetes Res, 2015;2015(0):547834.
      Species: Human
      Sample Type: Serum
    3. IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
      Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
      J Transl Med, 2015;13(0):381.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Interleukin 4, interleukin 6 and osteopontin-serological markers of head and neck malignancy in primary diagnostics: A pilot study.
      Authors: Aderhold C, Grobschmidt G, Sauter A, Faber A, Hormann K, Schultz J
      Oncol Lett, 2014;8(3):1112-1118.
      Species: Human
      Sample Type: Serum
    5. Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy.
      Authors: Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J
      PLoS ONE, 2014;9(6):e99612.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Molecular mechanisms of neutrophil dysfunction in glycogen storage disease type Ib.
      Authors: Jun H, Weinstein D, Lee Y, Mansfield B, Chou J
      Blood, 2014;123(18):2843-53.
      Species: Human
      Sample Type: Serum
    7. Influence of interleukin-4 gene polymorphisms and interleukin-4 serum level on susceptibility and severity of rheumatoid arthritis in Egyptian population.
      Authors: Hussein Y, El-Shal A, Rezk N, Abdel Galil S, Alzahrani S
      Cytokine, 2013;61(3):849-55.
      Species: Human
      Sample Type: Serum
    8. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
      Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
      Leukemia, 2011;25(1):161-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Shaping of iNKT cell repertoire after unrelated cord blood transplantation.
      Authors: Beziat V, Nguyen S, Exley M, Achour A, Simon T, Chevallier P, Sirvent A, Vigouroux S, Debre P, Rio B, Vieillard V, Lubrano-Berthelier C, Alatrakchi N
      Clin. Immunol., 2010;135(3):364-73.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
      Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
      J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
      Authors: Mabilleau G, Sabokbar A
      PLoS ONE, 2009;4(1):e4173.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
      Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
      Int. Immunol., 2008;20(10):1321-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
      Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
      Eur. J. Immunol., 2008;38(4):1012-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
      Authors: Miller R, Sadhukhan R, Wu C
      J. Immunol. Methods, 2008;334(1):134-41.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
      Authors: Lin YL, Liang YC, Chiang BL
      J. Leukoc. Biol., 2007;82(6):1473-80.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
      Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
      J. Allergy Clin. Immunol., 2007;120(5):1118-25.
      Species: Human
      Sample Type: BALF
    17. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
      Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
      AIDS Res. Hum. Retroviruses, 2007;23(7):886-90.
      Species: Human
      Sample Type: Plasma
    18. 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
      Authors: Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR
      Cytokine, 2007;40(2):128-34.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Decreased plasma CXCL16/SR-PSOX concentration is associated with coronary artery disease.
      Authors: Sheikine Y, Bang CS, Nilsson L, Samnegard A, Hamsten A, Jonasson L, Eriksson P, Sirsjo A
      Atherosclerosis, 2006;188(2):462-6.
      Species: Human
      Sample Type: Serum
    20. The potential of adiponectin in driving arthritis.
      Authors: Ehling A, Schaffler A, Herfarth H, Tarner IH, Anders S, Distler O, Paul G, Distler J, Gay S, Scholmerich J, Neumann E, Muller-Ladner U
      J. Immunol., 2006;176(7):4468-78.
      Species: Human
      Sample Type: Cell Culture Supernates
    21. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
      Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
      Gut, 2006;56(2):243-52.
      Species: Human
      Sample Type: Cell Culture Supernates
    22. Influence of eotaxin 67G>A polymorphism on plasma eotaxin concentrations in myocardial infarction survivors and healthy controls.
      Authors: Sheikine Y, Olsen B, Gharizadeh B, Jatta K, Tornvall P, Ghaderi M
      Atherosclerosis, 2006;189(2):458-63.
      Species: Human
      Sample Type: Serum
    23. Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.
      Authors: Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M
      Am. J. Respir. Crit. Care Med., 2006;173(12):1370-6.
      Species: Human
      Sample Type: Serum
    24. Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma.
      Authors: Yoshikawa H, Sato K, Edahiro S, Furukawa Y, Maruta T, Iwasa K, Watanabe H, Takaoka S, Suzuki Y, Takamori M, Yamada M
      J. Neuroimmunol., 2006;175(1):169-75.
      Species: Human
      Sample Type: Serum
    25. Detection of interleukin 10 in cerebrospinal fluid of patients with subacute sclerosing panencephalitis.
      Authors: Mistchenko AS, Fornari MC, Viegas M, Barrero PR, Diez RA
      J. Neurovirol., 2005;11(1):66-9.
      Species: Human
      Sample Type: CSF
    26. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
      Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
      Microbes Infect., 2005;7(3):427-36.
      Species: Human
      Sample Type: Serum
    27. Effect of piceatannol in human monocyte-derived dendritic cells in vitro.
      Authors: Takei M, Umeyama A, Arihara S, Matsumoto H
      J Pharm Sci, 2005;94(5):974-82.
      Species: Human
      Sample Type: Cell Culture Supernates
    28. Respiratory syncytial virus and other respiratory viruses during the first 3 months of life promote a local TH2-like response.
      Authors: Kristjansson S, Bjarnarson SP, Wennergren G, Palsdottir AH, Arnadottir T, Haraldsson A, Jonsdottir I
      J. Allergy Clin. Immunol., 2005;116(4):805-11.
      Species: Human
      Sample Type: Tissue Secretion
    29. Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
      Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
      J. Androl., 2004;25(2):250-4.
      Species: Human
      Sample Type: Seminal Plasma
    30. Cytokines evaluation in nasal lavage of allergic children after Bacillus clausii administration: a pilot study.
      Authors: Ciprandi G, Tosca MA, Milanese M, Caligo G, Ricca V
      Pediatr Allergy Immunol, 2004;15(2):148-51.
      Species: Human
      Sample Type: Nasal Lavage
    31. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
      Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
      J. Leukoc. Biol., 2003;73(5):584-90.
      Species: Human
      Sample Type: Cell Culture Supernates
    32. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
      Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
      Int. J. Cancer, 2002;101(2):183-9.
      Species: Human
      Sample Type: Ascites Fluid
    33. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
      Authors: D&amp;amp;apos;Ambrosio D
      Eur. J. Immunol., 2002;32(10):2934-43.
      Species: Human
      Sample Type: Cell Culture Supernates
    34. An analysis of acute changes in interleukin-6 levels after treatment of hepatitis C with consensus interferon.
      Authors: Cotler SJ, Reddy KR, McCone J, Wolfe DL, Liu A, Craft TR, Ferris MW, Conrad AJ, Albrecht J, Morrissey M, Ganger DR, Rosenblate H, Blatt LM, Jensen DM, Taylor MW
      J. Interferon Cytokine Res., 2001;21(12):1011-9.
      Species: Human
      Sample Type: Serum
    35. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
      Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
      J. Leukoc. Biol., 2001;70(4):617-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    36. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
      Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
      J. Exp. Med., 2000;192(2):219-26.
      Species: Human
      Sample Type: Cell Culture Supernates
    37. High versus low basal cortisol secretion in asymptomatic, medication-free HIV-infected men: differential effects of severe life stress on parameters of immune status.
      Authors: Petitto JM, Leserman J, Perkins DO, Stern RA, Silva SG, Gettes D, Zheng B, Folds JD, Golden RN, Evans DL
      Behav Med, 2000;25(4):143-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    38. Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells.
      Authors: Manetti, R, Parronchi, P, Giudizi, M G, Piccinni, M P, Maggi, E, Trinchieri, G, Romagnani, S
      J Exp Med, 1993;177(4):1199-204.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all 38 Citations
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    FAQs

    1. Why is plasma a validated sample type for the Human IL-4 Quantikine® ELISA (Catalog # D4050), but not in the high sensitivity version (Catalog # HS400)?

      • The recovery and linearity obtained with plasma samples assayed with the high sensitivity kit HS400 show too much variability and did not meet our specifications, unlike the D4050 which shows reliable recovery and linearity data with plasma samples.

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