Human IL-4 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
S4050
PD4050
D4050
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IL-4 ELISA Kit ELISA
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Product Details
Procedure
Citations (44)
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Human IL-4 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)
Sensitivity
10 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-4 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human IL-4 accurately. Results obtained using natural human IL-4 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-4.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 159 538 1092 169 503 1032
Standard Deviation 11.4 27.8 67.2 16.8 49.3 83
CV% 7.2 5.2 6.2 9.9 9.8 8

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 170 510 1110 132 411 865
Standard Deviation 6.7 17.5 58.5 9.8 32.1 52.8
CV% 3.9 3.4 5.3 7.4 7.8 6.1

Recovery

The recovery of human IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 98 90-109
Citrate Plasma (n=10) 94 85-109
EDTA Plasma (n=10) 100 90-123
Heparin Plasma (n=10) 95 84-121
Serum (n=10) 98 82-111

Linearity

To assess the linearity of the assay, the following biological samples, spiked with high concentrations of IL-4, were diluted with the appropriate Calibrator Diluent and then assayed.
Human IL-4 ELISA Linearity

Data Examples

Human IL-4 ELISA Cell Culture Supernate Standard Curve

Human IL-4 ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-4

IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.

Long Name:
Interleukin 4
Entrez Gene IDs:
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names:
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 3 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-4 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. NT-proBNP and CA 125 levels are associated with increased pro-inflammatory cytokines in coronary sinus serum of patients with chronic heart failure
    Authors: AE Stanciu, MM Stanciu, RG Vatasescu
    Cytokine, 2018;111(0):13-19.
    Species: Human
    Sample Types: Serum
  2. Oxidative Stress Markers Patients with Parotid Gland Tumors: A Pilot Study
    Authors: P Sowa, M Misiolek, B Pasinski, G Bartosz, M Soszynski, M Adamczyk-S, I Sadowska-B
    Biomed Res Int, 2018;2018(0):4340871.
    Species: Human
    Sample Types: Serum
  3. The effects of highly active antiretroviral therapy on the serum levels of pro-inflammatory and anti-inflammatory cytokines in HIV infected subjects
    Authors: FN Osuji, CC Onyenekwe, JE Ahaneku, NR Ukibe
    J. Biomed. Sci., 2018;25(1):88.
    Species: Human
    Sample Types: Plasma
  4. Altered levels of memory T cell subsets and common ?c cytokines in Strongyloides stercoralis infection and partial reversal following anthelmintic treatment
    Authors: A Rajamanick, S Munisankar, Y Bhootra, CK Dolla, K Thiruvenga, TB Nutman, S Babu
    PLoS Negl Trop Dis, 2018;12(5):e0006481.
    Species: Human
    Sample Types: Plasma
  5. Association between cytokine profile and transcription factors produced by T cells subsets in early and late-onset preeclampsia
    Authors: VR Ribeiro, M Romao-Veig, GG Romagnoli, ML Matias, PR Nunes, VTM Borges, JC Peracoli, MTS Peracoli
    Immunology, 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  6. Profiling of cytokines, chemokines and other soluble proteins as a potential biomarker in colorectal cancer and polyps
    Authors: NA Johdi, L Mazlan, I Sagap, R Jamal
    Cytokine, 2017;99(0):35-42.
    Species: Human
    Sample Types: Serum
  7. Human Basophils Modulate Plasma Cell Differentiation and Maturation
    Authors: Almut Meyer-Bahl
    J. Immunol., 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. The Association between Serum Cytokines and Damage to Large and Small Nerve Fibers in Diabetic Peripheral Neuropathy.
    Authors: Magrinelli F, Briani C, Romano M, Ruggero S, Toffanin E, Triolo G, Peter G, Praitano M, Lauriola M, Zanette G, Tamburin S
    J Diabetes Res, 2015;2015(0):547834.
    Species: Human
    Sample Types: Serum
  9. IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
    Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
    J Transl Med, 2015;13(0):381.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Molecular mechanisms of neutrophil dysfunction in glycogen storage disease type Ib.
    Authors: Jun H, Weinstein D, Lee Y, Mansfield B, Chou J
    Blood, 2014;123(18):2843-53.
    Species: Human
    Sample Types: Serum
  11. Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy.
    Authors: Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J
    PLoS ONE, 2014;9(6):e99612.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Interleukin 4, interleukin 6 and osteopontin-serological markers of head and neck malignancy in primary diagnostics: A pilot study.
    Authors: Aderhold C, Grobschmidt G, Sauter A, Faber A, Hormann K, Schultz J
    Oncol Lett, 2014;8(3):1112-1118.
    Species: Human
    Sample Types: Serum
  13. Influence of interleukin-4 gene polymorphisms and interleukin-4 serum level on susceptibility and severity of rheumatoid arthritis in Egyptian population.
    Authors: Hussein Y, El-Shal A, Rezk N, Abdel Galil S, Alzahrani S
    Cytokine, 2013;61(3):849-55.
    Species: Human
    Sample Types: Serum
  14. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
    Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
    Leukemia, 2011;25(1):161-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Shaping of iNKT cell repertoire after unrelated cord blood transplantation.
    Authors: Beziat V, Nguyen S, Exley M, Achour A, Simon T, Chevallier P, Sirvent A, Vigouroux S, Debre P, Rio B, Vieillard V, Lubrano-Berthelier C, Alatrakchi N
    Clin. Immunol., 2010;135(3):364-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
    Authors: Mabilleau G, Sabokbar A
    PLoS ONE, 2009;4(1):e4173.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
    Authors: Miller R, Sadhukhan R, Wu C
    J. Immunol. Methods, 2008;334(1):134-41.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
    Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
    Int. Immunol., 2008;20(10):1321-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008;38(4):1012-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
    Authors: Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR
    Cytokine, 2007;40(2):128-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
    Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
    AIDS Res. Hum. Retroviruses, 2007;23(7):886-90.
    Species: Human
    Sample Types: Plasma
  23. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
    Authors: Lin YL, Liang YC, Chiang BL
    J. Leukoc. Biol., 2007;82(6):1473-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
    Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
    J. Allergy Clin. Immunol., 2007;120(5):1118-25.
    Species: Human
    Sample Types: BALF
  25. The potential of adiponectin in driving arthritis.
    Authors: Ehling A, Schaffler A, Herfarth H, Tarner IH, Anders S, Distler O, Paul G, Distler J, Gay S, Scholmerich J, Neumann E, Muller-Ladner U
    J. Immunol., 2006;176(7):4468-78.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Decreased plasma CXCL16/SR-PSOX concentration is associated with coronary artery disease.
    Authors: Sheikine Y, Bang CS, Nilsson L, Samnegard A, Hamsten A, Jonasson L, Eriksson P, Sirsjo A
    Atherosclerosis, 2006;188(2):462-6.
    Species: Human
    Sample Types: Serum
  27. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
    Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
    Gut, 2006;56(2):243-52.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.
    Authors: Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M
    Am. J. Respir. Crit. Care Med., 2006;173(12):1370-6.
    Species: Human
    Sample Types: Serum
  29. Influence of eotaxin 67G>A polymorphism on plasma eotaxin concentrations in myocardial infarction survivors and healthy controls.
    Authors: Sheikine Y, Olsen B, Gharizadeh B, Jatta K, Tornvall P, Ghaderi M
    Atherosclerosis, 2006;189(2):458-63.
    Species: Human
    Sample Types: Serum
  30. Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma.
    Authors: Yoshikawa H, Sato K, Edahiro S, Furukawa Y, Maruta T, Iwasa K, Watanabe H, Takaoka S, Suzuki Y, Takamori M, Yamada M
    J. Neuroimmunol., 2006;175(1):169-75.
    Species: Human
    Sample Types: Serum
  31. Detection of interleukin 10 in cerebrospinal fluid of patients with subacute sclerosing panencephalitis.
    Authors: Mistchenko AS, Fornari MC, Viegas M, Barrero PR, Diez RA
    J. Neurovirol., 2005;11(1):66-9.
    Species: Human
    Sample Types: CSF
  32. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
    Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
    Microbes Infect., 2005;7(3):427-36.
    Species: Human
    Sample Types: Serum
  33. Respiratory syncytial virus and other respiratory viruses during the first 3 months of life promote a local TH2-like response.
    Authors: Kristjansson S, Bjarnarson SP, Wennergren G, Palsdottir AH, Arnadottir T, Haraldsson A, Jonsdottir I
    J. Allergy Clin. Immunol., 2005;116(4):805-11.
    Species: Human
    Sample Types: Tissue Secretion
  34. Effect of piceatannol in human monocyte-derived dendritic cells in vitro.
    Authors: Takei M, Umeyama A, Arihara S, Matsumoto H
    J Pharm Sci, 2005;94(5):974-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Cytokines evaluation in nasal lavage of allergic children after Bacillus clausii administration: a pilot study.
    Authors: Ciprandi G, Tosca MA, Milanese M, Caligo G, Ricca V
    Pediatr Allergy Immunol, 2004;15(2):148-51.
    Species: Human
    Sample Types: Nasal Lavage
  36. Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
    Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
    J. Androl., 2004;25(2):250-4.
    Species: Human
    Sample Types: Seminal Plasma
  37. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003;73(5):584-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
    Authors: D&amp;amp;apos;Ambrosio D
    Eur. J. Immunol., 2002;32(10):2934-43.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
    Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
    Int. J. Cancer, 2002;101(2):183-9.
    Species: Human
    Sample Types: Ascites Fluid
  40. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
    Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
    J. Leukoc. Biol., 2001;70(4):617-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. An analysis of acute changes in interleukin-6 levels after treatment of hepatitis C with consensus interferon.
    Authors: Cotler SJ, Reddy KR, McCone J, Wolfe DL, Liu A, Craft TR, Ferris MW, Conrad AJ, Albrecht J, Morrissey M, Ganger DR, Rosenblate H, Blatt LM, Jensen DM, Taylor MW
    J. Interferon Cytokine Res., 2001;21(12):1011-9.
    Species: Human
    Sample Types: Serum
  42. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
    J. Exp. Med., 2000;192(2):219-26.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. High versus low basal cortisol secretion in asymptomatic, medication-free HIV-infected men: differential effects of severe life stress on parameters of immune status.
    Authors: Petitto JM, Leserman J, Perkins DO, Stern RA, Silva SG, Gettes D, Zheng B, Folds JD, Golden RN, Evans DL
    Behav Med, 2000;25(4):143-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells.
    Authors: Manetti, R, Parronchi, P, Giudizi, M G, Piccinni, M P, Maggi, E, Trinchieri, G, Romagnani, S
    J Exp Med, 1993;177(4):1199-204.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

  1. Why is plasma a validated sample type for the Human IL-4 Quantikine® ELISA (Catalog # D4050), but not in the high sensitivity version (Catalog # HS400)?

    • The recovery and linearity obtained with plasma samples assayed with the high sensitivity kit HS400 show too much variability and did not meet our specifications, unlike the D4050 which shows reliable recovery and linearity data with plasma samples.

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Human IL-4 Quantikine ELISA Kit
By Anonymous on 07/12/2018
Sample Tested: 293T human embryonic kidney cell line

Human IL-4 Quantikine ELISA Kit
By Anonymous on 04/24/2018
Sample Tested: CHO Chinese hamster ovary cell line