• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
  • Sensitivity
    15 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-17
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC21, Quantikine Immunoassay Control Group 4 - Please Inquire
Product Summary
The Quantikine Human IL-17 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-17 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-17 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-17 accurately. Results obtained using natural human IL-17 showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-17.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean1317361480125408794
Standard Deviation5.834.661.29.834.355.6
CV%4.44.74.17.88.47

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean107604121776.6272549
Standard Deviation7.82563.36.523.443.9
CV%7.34.15.28.58.68

Recovery

The recovery of human IL-17 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 97 88-112
Citrate Plasma (n=5) 105 92-116
EDTA Plasma (n=5) 101 90-110
Heparin Plasma (n=5) 101 93-109
Serum (n=5) 98 90-105
Linearity
To assess the linearity of the assay, five samples spiked with high concentrations of IL-17 were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-17 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-17/IL-17A
The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.
    • Long Name
      Interleukin 17
    • Entrez Gene IDs
      3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine);
    • Alternate Names
      CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    10.   Aspirate and wash 3 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    45 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab
      Authors: U Bughani, A Saha, A Kuriakose, R Nair, RB Sadashivar, R Venkataram, S Patel, AT Deshchougu, SK S, E Montero, HV Pai, DV Palanivelu, R Melarkode, P Nair
      PLoS ONE, 2017;12(7):e0180088.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Lacrimal gland-derived IL-22 regulates IL-17-mediated ocular mucosal inflammation
      Authors: YW Ji, SK Mittal, HS Hwang, EJ Chang, JH Lee, Y Seo, A Yeo, H Noh, HS Lee, SK Chauhan, HK Lee
      Mucosal Immunol, 2017;0(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. High serum resistin associates with intrahepatic inflammation and necrosis: an index of disease severity for patients with chronic HBV infection
      Authors: Z Meng, Y Zhang, Z Wei, P Liu, J Kang, Y Zhang, D Ma, C Ke, Y Chen, J Luo, Z Gong
      BMC Gastroenterol, 2017;17(1):6.
      Species: Human
      Sample Type: Serum
    4. Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol
      J Exp Clin Cancer Res, 2016;35(1):118.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Expression of human gene coding ROR?T receptor depends on the Sp2 transcription factor
      Authors: Marcin Ratajewski
      J Leukoc Biol, 2016;0(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. CCL20-CCR6 Cytokine Network Facilitate Treg Activity in Advanced Grades and Metastatic Variants of Hepatocellular Carcinoma.
      Authors: Li W, Liu H
      Scand J Immunol, 2015;83(1):33-7.
      Species: Human
      Sample Type: Tissue Homogenates
    7. The impact of concomitant pulmonary infection on immune dysregulation in Pneumocystis jirovecii pneumonia.
      Authors: Chou C, Lin F, Tsai H, Chang S
      BMC Pulm Med, 2014;14(0):182.
      Species: Human
      Sample Type: BALF
    8. The pro-inflammatory potential of T cells in juvenile-onset systemic lupus erythematosus.
      Authors: Ballantine L, Ong J, Midgley A, Watson L, Flanagan B, Beresford M
      Pediatr Rheumatol Online J, 2014;12(0):4.
      Species: Human
      Sample Type: Plasma
    9. Live attenuated B. pertussis BPZE1 rescues the immune functions of Respiratory Syncytial virus infected human dendritic cells by promoting Th1/Th17 responses.
      Authors: Schiavoni I, Fedele G, Quattrini A, Bianco M, Schnoeller C, Openshaw P, Locht C, Ausiello C
      PLoS ONE, 2014;9(6):e100166.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Overexpression of IL-17RC associated with ocular sarcoidosis.
      Authors: Wu W, Jin M, Wang Y, Liu B, Shen D, Chen P, Hannes S, Li Z, Hirani S, Jawad S, Sen H, Chan C, Nussenblatt R, Wei L
      J Transl Med, 2014;12(0):152.
      Species: Human
      Sample Type: Serum
    11. Exogenous IFN-beta regulates the RANKL-c-Fos-IFN-beta signaling pathway in the collagen antibody-induced arthritis model.
      Authors: Zhao R, Chen N, Zhou X, Miao P, Hu C, Qian L, Yu Q, Zhang J, Nie H, Chen X, Li P, Xu R, Xiao L, Zhang X, Liu J, Zhang D
      J Transl Med, 2014;12(1):330.
      Species: Human
      Sample Type: Serum
    12. The systemic cytokine environment is permanently altered in multiple myeloma.
      Authors: Zheng M, Zhang Z, Bemis K, Belch A, Pilarski L, Shively J, Kirshner J
      PLoS ONE, 2013;8(3):e58504.
      Species: Human
      Sample Type: Plasma
    13. Elevated serum levels of interleukin-17A in children with autism.
      Authors: Al-Ayadhi LY, Mostafa GA
      J Neuroinflammation, 2012;9(1):158.
      Species: Human
      Sample Type: Serum
    14. Preclinical Evaluation of Local JAK1 and JAK2 Inhibition in Cutaneous Inflammation.
      Authors: Fridman JS, Scherle PA, Collins R, Burn T, Neilan CL, Hertel D, Contel N, Haley P, Thomas B, Shi J, Collier P, Rodgers JD, Shepard S, Metcalf B, Hollis G, Newton RC, Yeleswaram S, Friedman SM, Vaddi K
      J. Invest. Dermatol., 2011;131(9):1838-44.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
      Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
      Clin. Cancer Res., 2011;17(24):7529-38.
      Species: Human
      Sample Type: Serum
    16. Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD.
      Authors: Doe C, Bafadhel M, Siddiqui S, Desai D, Mistry V, Rugman P, McCormick M, Woods J, May R, Sleeman MA, Anderson IK, Brightling CE
      Chest, 2011;138(5):1140-7.
      Species: Human
      Sample Type: Sputum
    17. Subnormal cytokine profile in the tear fluid of keratoconus patients.
      Authors: Jun AS, Cope L, Speck C, Feng X, Lee S, Meng H, Hamad A, Chakravarti S
      PLoS ONE, 2011;6(1):e16437.
      Species: Human
      Sample Type: Tears
    18. Cytokine Response Patterns in Severe Pandemic 2009 H1N1 and Seasonal Influenza among Hospitalized Adults.
      Authors: Lee N, Wong CK, Chan PK, Chan MC, Wong RY, Lun SW, Ngai KL, Lui GC, Wong BC, Lee SK, Choi KW, Hui DS
      PLoS ONE, 2011;6(10):e26050.
      Species: Human
      Sample Type: Plasma
    19. Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneration.
      Authors: Liu B, Wei L, Meyerle C, Tuo J, Sen HN, Li Z, Chakrabarty S, Agron E, Chan CC, Klein ML, Chew E, Ferris F, Nussenblatt RB
      J Transl Med, 2011;9(0):111.
      Species: Human
      Sample Type: Serum
    20. Identification of human NK17/NK1 cells.
      Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
      PLoS ONE, 2011;6(10):e26780.
      Species: Human
      Sample Type: Cell Culture Supernates
    21. Kinetics of circulating Th17 cytokines and adipokines in psoriasis patients.
      Authors: Nakajima H, Nakajima K, Tarutani M, Morishige R, Sano S
      Arch. Dermatol. Res., 2011;303(6):451-5.
      Species: Human
      Sample Type: Serum
    22. Regulating human Th17 cells via differential expression of IL-1 receptor.
      Authors: Lee WW, Kang SW, Choi J
      Blood, 2010;115(3):530-40.
      Species: Human
      Sample Type: Cell Culture Supernates
    23. Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells.
      Authors: Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB
      Allergy, 2010;65(5):581-9.
      Species: Human
      Sample Type: Nasal Lavage
    24. Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
      Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
      J. Allergy Clin. Immunol., 2010;126(5):962.
      Species: Human
      Sample Type: Tissue Homogenates
    25. An antiinflammatory dietary mix modulates inflammation and oxidative and metabolic stress in overweight men: a nutrigenomics approach.
      Authors: Bakker GC, van Erk MJ, Pellis L, Wopereis S, Rubingh CM, Cnubben NH, Kooistra T, van Ommen B, Hendriks HF
      Am. J. Clin. Nutr., 2010;91(4):1044-59.
      Species: Human
      Sample Type: Plasma
    26. B7-h3 augments the inflammatory response and is associated with human sepsis.
      Authors: Zhang G, Wang J, Kelly J
      J. Immunol., 2010;185(6):3677-84.
      Species: Human
      Sample Type: Plasma
    27. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
      Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
      Transplantation, 2009;88(9):1109-16.
      Species: Human
      Sample Type: Urine
    28. Serum interleukin-17 levels are related to clinical severity in allergic rhinitis.
      Authors: Ciprandi G, De Amici M, Murdaca G, Fenoglio D, Ricciardolo F, Marseglia G, Tosca M
      Allergy, 2009;64(9):1375-8.
      Species: Human
      Sample Type: Serum
    29. Interleukin 17: an unlikely marker of acute coronary syndrome?
      Authors: Patel KD, Murphy RT, White M, Gasparro D, Kelleher DP, Ryan T, McManus R, Ryan AW
      Atherosclerosis, 2009;205(1):33-4.
      Species: Human
      Sample Type: Serum
    30. Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
      Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
      J. Exp. Med., 2009;206(3):535-48.
      Species: Human
      Sample Type: Cell Culture Supernates
    31. Intervention of an inflammation amplifier, triggering receptor expressed on myeloid cells 1, for treatment of autoimmune arthritis.
      Authors: Murakami Y, Akahoshi T, Aoki N, Toyomoto M, Miyasaka N, Kohsaka H
      Arthritis Rheum., 2009;60(6):1615-23.
      Species: Human
      Sample Type: Serum
    32. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
      Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
      J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
      Species: Human
      Sample Type: Cell Culture Supernates
    33. IL-17 induces monocyte migration in rheumatoid arthritis.
      Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
      J. Immunol., 2009;182(6):3884-91.
      Species: Human
      Sample Type: Synovial Fluid
    34. The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
      Authors: Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R
      Eur. J. Immunol., 2009;39(12):3570-81.
      Species: Human
      Sample Type: Serum
    35. Memory Th-17 cells specific for C. albicans are persistent in human peripheral blood.
      Authors: Zhou M, Yang B, Ma R, Wu C
      Immunol. Lett., 2008;118(1):72-81.
      Species: Human
      Sample Type: Cell Culture Supernates
    36. Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides.
      Authors: Jandus C, Bioley G, Rivals JP, Dudler J, Speiser D, Romero P
      Arthritis Rheum., 2008;58(8):2307-17.
      Species: Human
      Sample Type: Cell Culture Supernates
    37. Linking genetic susceptibility to Crohn's disease with Th17 cell function: IL-22 serum levels are increased in Crohn's disease and correlate with disease activity and IL23R genotype status.
      Authors: Schmechel S, Konrad A, Diegelmann J, Glas J, Wetzke M, Paschos E, Lohse P, Goke B, Brand S
      Inflamm. Bowel Dis., 2008;14(2):204-12.
      Species: Human
      Sample Type: Serum
    38. Chronically inflamed human tissues are infiltrated by highly differentiated Th17 lymphocytes.
      Authors: Pene J, Chevalier S, Preisser L, Venereau E, Guilleux MH, Ghannam S, Moles JP, Danger Y, Ravon E, Lesaux S, Yssel H, Gascan H
      J. Immunol., 2008;180(0):7423.
      Species: Human
      Sample Type: Cell Culture Supernates
    39. Serum levels of MMP-3 and cathepsin K in patients with ankylosing spondylitis: effect of TNFalpha antagonist therapy.
      Authors: Wendling D, Cedoz JP, Racadot E
      Joint Bone Spine, 2008;75(5):559-62.
      Species: Human
      Sample Type: Serum
    40. IL-22 induces lipopolysaccharide-binding protein in hepatocytes: a potential systemic role of IL-22 in Crohn's disease.
      Authors: Wolk K, Witte E, Hoffmann U, Doecke WD, Endesfelder S, Asadullah K, Sterry W, Volk HD, Wittig BM, Sabat R
      J. Immunol., 2007;178(9):5973-81.
      Species: Human
      Sample Type: Cell Culture Supernates
    41. IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
      Authors: Rao DA, Tracey KJ, Pober JS
      J. Immunol., 2007;179(10):6536-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    42. IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production.
      Authors: Vaknin-Dembinsky A, Balashov K, Weiner HL
      J. Immunol., 2006;176(12):7768-74.
      Species: Human
      Sample Type: Cell Culture Supernates
    43. Levels of interleukin-17 in gingival crevicular fluid and in supernatants of cellular cultures of gingival tissue from patients with chronic periodontitis.
      Authors: Vernal R, Dutzan N, Chaparro A, Puente J, Antonieta Valenzuela M, Gamonal J
      J. Clin. Periodontol., 2005;32(4):383-9.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    44. Cytokine profile in Behcet's disease patients. Relationship with disease activity.
      Authors: Hamzaoui K, Hamzaoui A, Guemira F, Bessioud M, Hamza M, Ayed K
      Scand. J. Rheumatol., 2002;31(4):205-10.
      Species: Human
      Sample Type: Serum
    45. Stimulation of 92-kd gelatinase (matrix metalloproteinase 9) production by interleukin-17 in human monocyte/macrophages: a possible role in rheumatoid arthritis.
      Authors: Jovanovic DV, Martel-Pelletier J, Di Battista JA, Mineau F, Jolicoeur FC, Benderdour M, Pelletier JP
      Arthritis Rheum., 2000;43(5):1134-44.
      Species: Human
      Sample Type: Synovial Fluid
    Expand to show all 45 Citations
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     Human IL-17 Quantikine ELISA Kit D1700
    : Human IL-17 Quantikine ELISA Kit [D1700]

    Summary

    Sample TestedT cells
    SpeciesHuman

    Other Experimental Details

    Other Experimental DetailsStandard curve is shown.
    Buffer: kit dilution buffer
    Dilution: 1/100

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