Human IL-17 Quantikine ELISA Kit

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PD1700
S1700
D1700
Control Products Available
Human IL-17/IL-17A ELISA Cell Culture Supernate Standard Curve
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Product Details
Procedure
Citations (63)
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Human IL-17 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
Sensitivity
15 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-17
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-17 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-17 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-17 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-17 accurately. Results obtained using natural human IL-17 showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-17.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 107 604 1217 76.6 272 549
Standard Deviation 7.8 25 63.3 6.5 23.4 43.9
CV% 7.3 4.1 5.2 8.5 8.6 8

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 131 736 1480 125 408 794
Standard Deviation 5.8 34.6 61.2 9.8 34.3 55.6
CV% 4.4 4.7 4.1 7.8 8.4 7

Recovery

The recovery of human IL-17 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 97 88-112
Citrate Plasma (n=5) 105 92-116
EDTA Plasma (n=5) 101 90-110
Heparin Plasma (n=5) 101 93-109
Serum (n=5) 98 90-105

Linearity

To assess the linearity of the assay, five samples spiked with high concentrations of IL-17 were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-17/IL-17A ELISA Linearity

Data Examples

Human IL-17/IL-17A ELISA Cell Culture Supernate Standard Curve

Human IL-17/IL-17A ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

Human Interleukin 17 (IL-17), also known as IL-17A and CTLA-8, is a 15-20 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines (1-5). Its alternate name, CTLA-8, originated from rodent studies where an activated hybridoma was created from the fusion of a mouse cytotoxic and a rat T cell lymphoma cell line. The molecule of interest in this study was assumed to have come from the mouse cytotoxic lymphocyte cell (thus the CTL designation), whereas, in fact, it was a rat lymphocyte molecule. Human IL-17/17A is synthesized as a 155 amino acid (aa) precursor that contains a 23 aa signal sequence and a 133 aa mature region that possesses a cysteine-knot fold (4-6). In both human and mouse, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 32-38 kDa disulfide-linked homodimer, and a 40-45 kDa covalent heterodimer with IL-17F (7-9). Most secreted IL-17A is in the form of the IL-17A:F heterodimer, however, the IL-17A:A homodimer is the most bioactive of the two forms (8). Mature human IL-17A is 61%, 74%, and 99% aa identical to mouse, porcine, and chimpanzee IL-17A, respectively (10-12). Mammalian cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27- gamma δ T cells, CD1+NK1.1- iNKT cells, and CD3- CD4+ LTi-like cells (9, 13-17). 

A high affinity receptor for human IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio (1, 18). IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily (2, 10, 15). IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30% aa identity with IL-17RA (19, 20). Both receptors are needed for IL-17A and IL-17A:F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA (1, 21, 22). 
IL-17 is best known for its participation in the recruitment and survival of neutrophils (14, 15, 23, 24, 25). Its induction was initially described to be the result of antigen stimulation of dendritic cells, resulting in IL-23 secretion. In a T cell receptor-independent event, IL-23 induces T cell production of IL-17 (14). Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and stem cell factor (membrane form), an effect that increases polymorphonuclear neutrophils (PMN) differentiation and production. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating PMN numbers (23). In the tissues, IL-17 would also seem to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-alpha, IL-1b and IL-6 production (26). TNF-alpha and IL-1b then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 (27). IL-17 further contributes to PMN influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability (14, 28). IL-17 effects are not limited to inflammation. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption (24). In conjunction with IL-4 and CD40L, IL-17A also promotes the generation of IgE secreting cells (29). And in white fat, IL-17A inhibits adipocyte differentiation from preadipocytes, and impairs glucose uptake by mature adipocytes (30).

Long Name:
Interleukin 17
Entrez Gene IDs:
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names:
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  10.   Aspirate and wash 3 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-17 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

63 Citations: Showing 1 - 10
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  1. lncRNA CASC2 downregulation participates in rheumatoid arthritis, and CASC2 overexpression promotes the apoptosis of fibroblast?like synoviocytes by downregulating IL?17
    Authors: C Liu, X Guo, S Bai, G Zeng, H Wang
    Mol Med Rep, 2020;0(0):.
    Species: Human
    Sample Types: Plasma
  2. Identification of Novel Molecular Markers of Human Th17 Cells
    Authors: A Sa?kowska, K Kara?, I Karwaciak, A Walczak-Dr, M Krawczyk, M Sobalska-K, J Dastych, M Ratajewski
    Cells, 2020;9(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Circulating IL-17A Levels in Postmenopausal Women with Primary Hyperparathyroidism
    Authors: E Dozio, E Passeri, E Vianello, S Palmieri, C Eller-Vain, M Corsi Roma, S Corbetta
    Mediators Inflamm., 2020;2020(0):3417329.
    Species: Human
    Sample Types: Plasma
  4. Plasma levels of TNF-?, IL-6, IFN-?, IL-12, IL-17, IL-22, and IL-23 in achalasia, eosinophilic esophagitis (EoE), and gastroesophageal reflux disease (GERD)
    Authors: S Clayton, E Cauble, A Kumar, N Patil, D Ledford, N Kolliputi, MF Lopes-Vire, D Castell, J Richter
    BMC Gastroenterol, 2019;19(1):28.
    Species: Human
    Sample Types: Plasma
  5. TH17 cells�expressing CD146 are significantly increased in patients with Systemic sclerosis
    Authors: A Gabsi, X Heim, A Dlala, A Gati, H Sakhri, A Abidi, S Amri, B Neili, AS Leroyer, A Bertaud, M Smiti Khan, F Said, MH Houman, B Granel, M Blot-Chaba, N Bardin, R Marrakchi
    Sci Rep, 2019;9(1):17721.
    Species: Human
    Sample Types: Serum
  6. Association between body composition and inflammation: A central role of IL-17 and IL-10 in diabetic and hypertensive elderly women
    Authors: IS Ribeiro, ÍS Pereira, DP Santos, DS Lopes, AO Prado, SPM Calado, CV Gonçalves, MPL Galantini, IPR Muniz, GS Santos, RAA Silva
    Exp. Gerontol., 2019;127(0):110734.
    Species: Human
    Sample Types: Plasma
  7. Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses
    Authors: H Yu, S Cui, Y Mei, Q Li, L Wu, S Duan, G Cai, H Zhu, B Fu, L Zhang, Z Feng, X Chen
    J Immunol Res, 2019;2019(0):2121849.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. LncRNA�CASC2 is upregulated in osteoarthritis and participates in the regulation of IL-17 expression and chondrocyte proliferation and apoptosis
    Authors: T Huang, J Wang, Y Zhou, Y Zhao, D Hang, Y Cao
    Biosci. Rep., 2019;0(0):.
    Species: Human
    Sample Types: Plasma
  9. IL-17A-associated IKK-? signaling induced TSLP production in epithelial cells of COPD patients
    Authors: G Anzalone, GD Albano, AM Montalbano, L Riccobono, A Bonanno, R Gagliardo, F Bucchieri, R Marchese, M Moscato, M Profita
    Exp. Mol. Med., 2018;50(10):131.
    Species: Human
    Sample Types: Sputum Supernates
  10. Digoxin, an Overlooked Agonist of ROR?/ROR?T
    Authors: K Kara?, A Sa?kowska, M Sobalska-K, A Walczak-Dr, D Strapagiel, J Dastych, RA Bachorz, M Ratajewski
    Front Pharmacol, 2018;9(0):1460.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Serum levels of IL-17 are elevated in patients with acute gouty arthritis
    Authors: Y Liu, Q Zhao, Y Yin, MA McNutt, T Zhang, Y Cao
    Biochem. Biophys. Res. Commun., 2018;497(3):897-902.
    Species: Human
    Sample Types: Serum
  12. BCG Vaccination Protects against Experimental Viral Infection in Humans through the Induction of Cytokines Associated with Trained Immunity
    Authors: RJW Arts, SJCFM Moorlag, B Novakovic, Y Li, SY Wang, M Oosting, V Kumar, RJ Xavier, C Wijmenga, LAB Joosten, CBEM Reusken, CS Benn, P Aaby, MP Koopmans, HG Stunnenber, R van Crevel, MG Netea
    Cell Host Microbe, 2018;23(1):89-100.e5.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Decreased interleukin-11 levels in the semen of infertile males
    Authors: L Qian
    Cytokine, 2018;108(0):57-59.
    Species: Human
    Sample Types: Semen
  14. Rheumatoid arthritis bone marrow environment supports Th17 response.
    Authors: Ewa Kuca-Warna, Weronika Kurowska, Monika Prochorec, Anna Radzikows, Tomasz Burakowsk, Urszula Skalska, Magdalena Massalska, Magdalena Pleba?czy, Barbara Ma?dyk-No, Iwona S?owi?ska, Robert Gasik, W?odzimierz Ma?li?ski
    Arthritis Research & Therapy, 2017;0(0):1478-6362.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab
    Authors: U Bughani, A Saha, A Kuriakose, R Nair, RB Sadashivar, R Venkataram, S Patel, AT Deshchougu, SK S, E Montero, HV Pai, DV Palanivelu, R Melarkode, P Nair
    PLoS ONE, 2017;12(7):e0180088.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. High serum resistin associates with intrahepatic inflammation and necrosis: an index of disease severity for patients with chronic HBV infection
    Authors: Z Meng, Y Zhang, Z Wei, P Liu, J Kang, Y Zhang, D Ma, C Ke, Y Chen, J Luo, Z Gong
    BMC Gastroenterol, 2017;17(1):6.
    Species: Human
    Sample Types: Serum
  17. Lacrimal gland-derived IL-22 regulates IL-17-mediated ocular mucosal inflammation
    Authors: YW Ji, SK Mittal, HS Hwang, EJ Chang, JH Lee, Y Seo, A Yeo, H Noh, HS Lee, SK Chauhan, HK Lee
    Mucosal Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Rheumatoid arthritis bone marrow environment supports Th17 response
    Authors: E Kuca-Warna, W Kurowska, M Prochorec-, A Radzikowsk, T Burakowski, U Skalska, M Massalska, M Pleba?czyk, B Ma?dyk-Now, I S?owi?ska, R Gasik, W Ma?li?ski
    Arthritis Res. Ther., 2017;19(1):274.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Increased interleukin (IL)-17 serum levels in patients with hidradenitis suppurativa: Implications for treatment with anti-IL-17 agents
    Authors: ?ukasz Matusiak
    J. Am. Acad. Dermatol, 2016;0(0):.
    Species: Human
    Sample Types: Serum
  20. Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol
    J Exp Clin Cancer Res, 2016;35(1):118.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Expression of human gene coding ROR?T receptor depends on the Sp2 transcription factor
    Authors: Marcin Ratajewski
    J Leukoc Biol, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. CCL20-CCR6 Cytokine Network Facilitate Treg Activity in Advanced Grades and Metastatic Variants of Hepatocellular Carcinoma.
    Authors: Li W, Liu H
    Scand J Immunol, 2015;83(1):33-7.
    Species: Human
    Sample Types: Tissue Homogenates
  23. Live attenuated B. pertussis BPZE1 rescues the immune functions of Respiratory Syncytial virus infected human dendritic cells by promoting Th1/Th17 responses.
    Authors: Schiavoni I, Fedele G, Quattrini A, Bianco M, Schnoeller C, Openshaw P, Locht C, Ausiello C
    PLoS ONE, 2014;9(6):e100166.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Overexpression of IL-17RC associated with ocular sarcoidosis.
    Authors: Wu W, Jin M, Wang Y, Liu B, Shen D, Chen P, Hannes S, Li Z, Hirani S, Jawad S, Sen H, Chan C, Nussenblatt R, Wei L
    J Transl Med, 2014;12(0):152.
    Species: Human
    Sample Types: Serum
  25. The impact of concomitant pulmonary infection on immune dysregulation in Pneumocystis jirovecii pneumonia.
    Authors: Chou C, Lin F, Tsai H, Chang S
    BMC Pulm Med, 2014;14(0):182.
    Species: Human
    Sample Types: BALF
  26. The pro-inflammatory potential of T cells in juvenile-onset systemic lupus erythematosus.
    Authors: Ballantine L, Ong J, Midgley A, Watson L, Flanagan B, Beresford M
    Pediatr Rheumatol Online J, 2014;12(0):4.
    Species: Human
    Sample Types: Plasma
  27. Exogenous IFN-beta regulates the RANKL-c-Fos-IFN-beta signaling pathway in the collagen antibody-induced arthritis model.
    Authors: Zhao R, Chen N, Zhou X, Miao P, Hu C, Qian L, Yu Q, Zhang J, Nie H, Chen X, Li P, Xu R, Xiao L, Zhang X, Liu J, Zhang D
    J Transl Med, 2014;12(1):330.
    Species: Human
    Sample Types: Serum
  28. The systemic cytokine environment is permanently altered in multiple myeloma.
    Authors: Zheng M, Zhang Z, Bemis K, Belch A, Pilarski L, Shively J, Kirshner J
    PLoS ONE, 2013;8(3):e58504.
    Species: Human
    Sample Types: Plasma
  29. Elevated serum levels of interleukin-17A in children with autism.
    Authors: Al-Ayadhi LY, Mostafa GA
    J Neuroinflammation, 2012;9(1):158.
    Species: Human
    Sample Types: Serum
  30. Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD.
    Authors: Doe C, Bafadhel M, Siddiqui S, Desai D, Mistry V, Rugman P, McCormick M, Woods J, May R, Sleeman MA, Anderson IK, Brightling CE
    Chest, 2011;138(5):1140-7.
    Species: Human
    Sample Types: Sputum
  31. Cytokine Response Patterns in Severe Pandemic 2009 H1N1 and Seasonal Influenza among Hospitalized Adults.
    Authors: Lee N, Wong CK, Chan PK, Chan MC, Wong RY, Lun SW, Ngai KL, Lui GC, Wong BC, Lee SK, Choi KW, Hui DS
    PLoS ONE, 2011;6(10):e26050.
    Species: Human
    Sample Types: Plasma
  32. Kinetics of circulating Th17 cytokines and adipokines in psoriasis patients.
    Authors: Nakajima H, Nakajima K, Tarutani M, Morishige R, Sano S
    Arch. Dermatol. Res., 2011;303(6):451-5.
    Species: Human
    Sample Types: Serum
  33. Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneration.
    Authors: Liu B, Wei L, Meyerle C, Tuo J, Sen HN, Li Z, Chakrabarty S, Agron E, Chan CC, Klein ML, Chew E, Ferris F, Nussenblatt RB
    J Transl Med, 2011;9(0):111.
    Species: Human
    Sample Types: Serum
  34. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011;6(10):e26780.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011;17(24):7529-38.
    Species: Human
    Sample Types: Serum
  36. Subnormal cytokine profile in the tear fluid of keratoconus patients.
    Authors: Jun AS, Cope L, Speck C, Feng X, Lee S, Meng H, Hamad A, Chakravarti S
    PLoS ONE, 2011;6(1):e16437.
    Species: Human
    Sample Types: Tears
  37. Preclinical Evaluation of Local JAK1 and JAK2 Inhibition in Cutaneous Inflammation.
    Authors: Fridman JS, Scherle PA, Collins R, Burn T, Neilan CL, Hertel D, Contel N, Haley P, Thomas B, Shi J, Collier P, Rodgers JD, Shepard S, Metcalf B, Hollis G, Newton RC, Yeleswaram S, Friedman SM, Vaddi K
    J. Invest. Dermatol., 2011;131(9):1838-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells.
    Authors: Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB
    Allergy, 2010;65(5):581-9.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  39. An antiinflammatory dietary mix modulates inflammation and oxidative and metabolic stress in overweight men: a nutrigenomics approach.
    Authors: Bakker GC, van Erk MJ, Pellis L, Wopereis S, Rubingh CM, Cnubben NH, Kooistra T, van Ommen B, Hendriks HF
    Am. J. Clin. Nutr., 2010;91(4):1044-59.
    Species: Human
    Sample Types: Plasma
  40. Regulating human Th17 cells via differential expression of IL-1 receptor.
    Authors: Lee WW, Kang SW, Choi J
    Blood, 2010;115(3):530-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
    Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
    J. Allergy Clin. Immunol., 2010;126(5):962.
    Species: Human
    Sample Types: Tissue Homogenates
  42. B7-h3 augments the inflammatory response and is associated with human sepsis.
    Authors: Zhang G, Wang J, Kelly J
    J. Immunol., 2010;185(6):3677-84.
    Species: Human
    Sample Types: Plasma
  43. Intervention of an inflammation amplifier, triggering receptor expressed on myeloid cells 1, for treatment of autoimmune arthritis.
    Authors: Murakami Y, Akahoshi T, Aoki N, Toyomoto M, Miyasaka N, Kohsaka H
    Arthritis Rheum., 2009;60(6):1615-23.
    Species: Human
    Sample Types: Serum
  44. The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
    Authors: Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R
    Eur. J. Immunol., 2009;39(12):3570-81.
    Species: Human
    Sample Types: Serum
  45. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Serum interleukin-17 levels are related to clinical severity in allergic rhinitis.
    Authors: Ciprandi G, De Amici M, Murdaca G, Fenoglio D, Ricciardolo F, Marseglia G, Tosca M
    Allergy, 2009;64(9):1375-8.
    Species: Human
    Sample Types: Serum
  47. Interleukin 17: an unlikely marker of acute coronary syndrome?
    Authors: Patel KD, Murphy RT, White M, Gasparro D, Kelleher DP, Ryan T, McManus R, Ryan AW
    Atherosclerosis, 2009;205(1):33-4.
    Species: Human
    Sample Types: Serum
  48. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
    Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
    Transplantation, 2009;88(9):1109-16.
    Species: Human
    Sample Types: Urine
  49. IL-17 induces monocyte migration in rheumatoid arthritis.
    Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
    J. Immunol., 2009;182(6):3884-91.
    Species: Human
    Sample Types: Synovial Fluid
  50. Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
    Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
    J. Exp. Med., 2009;206(3):535-48.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. Linking genetic susceptibility to Crohn's disease with Th17 cell function: IL-22 serum levels are increased in Crohn's disease and correlate with disease activity and IL23R genotype status.
    Authors: Schmechel S, Konrad A, Diegelmann J, Glas J, Wetzke M, Paschos E, Lohse P, Goke B, Brand S
    Inflamm. Bowel Dis., 2008;14(2):204-12.
    Species: Human
    Sample Types: Serum
  52. Chronically inflamed human tissues are infiltrated by highly differentiated Th17 lymphocytes.
    Authors: Pene J, Chevalier S, Preisser L, Venereau E, Guilleux MH, Ghannam S, Moles JP, Danger Y, Ravon E, Lesaux S, Yssel H, Gascan H
    J. Immunol., 2008;180(0):7423.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Serum levels of MMP-3 and cathepsin K in patients with ankylosing spondylitis: effect of TNFalpha antagonist therapy.
    Authors: Wendling D, Cedoz JP, Racadot E
    Joint Bone Spine, 2008;75(5):559-62.
    Species: Human
    Sample Types: Serum
  54. Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides.
    Authors: Jandus C, Bioley G, Rivals JP, Dudler J, Speiser D, Romero P
    Arthritis Rheum., 2008;58(8):2307-17.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Memory Th-17 cells specific for C. albicans are persistent in human peripheral blood.
    Authors: Zhou M, Yang B, Ma R, Wu C
    Immunol. Lett., 2008;118(1):72-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. IL-22 induces lipopolysaccharide-binding protein in hepatocytes: a potential systemic role of IL-22 in Crohn's disease.
    Authors: Wolk K, Witte E, Hoffmann U, Doecke WD, Endesfelder S, Asadullah K, Sterry W, Volk HD, Wittig BM, Sabat R
    J. Immunol., 2007;178(9):5973-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
    Authors: Rao DA, Tracey KJ, Pober JS
    J. Immunol., 2007;179(10):6536-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production.
    Authors: Vaknin-Dembinsky A, Balashov K, Weiner HL
    J. Immunol., 2006;176(12):7768-74.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. Levels of interleukin-17 in gingival crevicular fluid and in supernatants of cellular cultures of gingival tissue from patients with chronic periodontitis.
    Authors: Vernal R, Dutzan N, Chaparro A, Puente J, Antonieta Valenzuela M, Gamonal J
    J. Clin. Periodontol., 2005;32(4):383-9.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)
  60. Cytokine profile in Behcet's disease patients. Relationship with disease activity.
    Authors: Hamzaoui K, Hamzaoui A, Guemira F, Bessioud M, Hamza M, Ayed K
    Scand. J. Rheumatol., 2002;31(4):205-10.
    Species: Human
    Sample Types: Serum
  61. Stimulation of 92-kd gelatinase (matrix metalloproteinase 9) production by interleukin-17 in human monocyte/macrophages: a possible role in rheumatoid arthritis.
    Authors: Jovanovic DV, Martel-Pelletier J, Di Battista JA, Mineau F, Jolicoeur FC, Benderdour M, Pelletier JP
    Arthritis Rheum., 2000;43(5):1134-44.
    Species: Human
    Sample Types: Synovial Fluid
  62. VSIG-3 as a ligand of VISTA inhibits human T-cell function.
    Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
    Immunology, 0;156(1):74-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. Long-lasting effects of BCG vaccination on both heterologous Th1/Th17 responses and innate trained immunity.
    Authors: Kleinnijenhuis J, Quintin J, Preijers F, Benn C, Joosten L, Jacobs C, van Loenhout J, Xavier R, Aaby P, van der Meer J, van Crevel R, Netea M
    J Innate Immun, 0;6(2):152-8.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human IL-17 Quantikine ELISA Kit
By Anonymous on 10/26/2015
Application: Sample Tested: T cells Species: Human

Standard curve is shown.
Buffer: kit dilution buffer
Dilution: 1/100