Human IFN-gamma Quantikine ELISA Kit DIF50: R&D Systems

Human IFN-gamma Quantikine ELISA Kit

(109 citations)
(3 Reviews)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL)
  • Sensitivity
    8 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant human IFN-gamma
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC01-1, Quantikine Immunoassay Control Group 1 - Please Inquire
Product Summary
The Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure IFN-gamma levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring human IFN-gamma samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean79.221446678.7206457
Standard Deviation3.75.5136.113.117
CV%4.72.62.87.86.43.7

Recovery

The recovery of IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 93-109
EDTA Plasma (n=5) 98 88-111
Serum (n=5) 102 91-118
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IFN-gamma in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IFN-gamma Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-gamma
IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
    • Long Name
      Interferon gamma
    • Entrez Gene IDs
      3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline);
    • Alternate Names
      IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    109 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Frequency and role of NKp46 and NKG2A in hepatitis B virus infection
      Authors: T Yoshioka, T Tatsumi, T Miyagi, K Mukai, K Nishio, A Nishio, Y Yokoyama, T Suda, T Kegasawa, M Shigekawa, H Hikita, R Sakamori, T Takehara
      PLoS ONE, 2017;12(3):e0174103.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Myeloid-derived suppressor cells are increased and correlated with type 2 immune responses, malnutrition, inflammation, and poor prognosis in patients with breast cancer
      Authors: K Gonda, M Shibata, T Ohtake, Y Matsumoto, K Tachibana, N Abe, H Ohto, K Sakurai, S Takenoshit
      Oncol Lett, 2017;14(2):1766-1774.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome
      Authors: KC Clark, FA Fierro, EM Ko, NJ Walker, B Arzi, CG Tepper, H Dahlenburg, A Cicchetto, A Kol, L Marsh, WJ Murphy, N Fazel, DL Borjesson
      Stem Cell Res Ther, 2017;8(1):69.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Alterations in the Fecal Microbiota of Patients with HIV-1 Infection: An Observational Study in A Chinese Population
      Sci Rep, 2016;6(0):30673.
      Species: Human
      Sample Type: Serum
    5. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
      J Immunol, 2016;197(5):1621-30.
      Species: Human
      Sample Type: Serum
    6. WNT5A transforms intestinal CD8alphaalpha(+) IELs into an unconventional phenotype with pro-inflammatory features.
      Authors: Zhao D, Xu A, Dai Z, Peng J, Zhu M, Shen J, Zheng Q, Ran Z
      BMC Gastroenterol, 2015;15(0):173.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    7. CCL20-CCR6 Cytokine Network Facilitate Treg Activity in Advanced Grades and Metastatic Variants of Hepatocellular Carcinoma.
      Authors: Li W, Liu H
      Scand J Immunol, 2015;83(1):33-7.
      Species: Human
      Sample Type: Tissue Homogenates
    8. A Multidrug-resistant Engineered CAR T Cell for Allogeneic Combination Immunotherapy.
      Authors: Valton J, Guyot V, Marechal A, Filhol J, Juillerat A, Duclert A, Duchateau P, Poirot L
      Mol Ther, 2015;23(9):1507-18.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Comparative Multi-Donor Study of IFNgamma Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays.
      Authors: Hagen J, Zimmerman R, Goetz C, Bonnevier J, Houchins J, Reagan K, Kalyuzhny A
      Cells, 2015;4(1):84-95.
      Species: Human
      Sample Type: Whole Cells
    10. A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism.
      Authors: Mankad D, Dupuis A, Smile S, Roberts W, Brian J, Lui T, Genore L, Zaghloul D, Iaboni A, Marcon P, Anagnostou E
      Mol Autism, 2015;6(0):18.
      Species: Human
      Sample Type: Plasma
    11. Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy.
      Authors: Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J
      PLoS ONE, 2014;9(6):e99612.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. EBV-encoded miR-BART20-5p and miR-BART8 inhibit the IFN-gamma-STAT1 pathway associated with disease progression in nasal NK-cell lymphoma.
      Authors: Huang W, Lin C
      Am J Pathol, 2014;184(4):1185-97.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
      Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
      PLoS ONE, 2014;9(1):e86466.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Live attenuated B. pertussis BPZE1 rescues the immune functions of Respiratory Syncytial virus infected human dendritic cells by promoting Th1/Th17 responses.
      Authors: Schiavoni I, Fedele G, Quattrini A, Bianco M, Schnoeller C, Openshaw P, Locht C, Ausiello C
      PLoS ONE, 2014;9(6):e100166.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma.
      Authors: Han L, Liu F, Li R, Li Z, Chen X, Zhou Z, Zhang X, Hu T, Zhang Y, Young K, Sun S, Wen J, Zhang M
      Oncol Lett, 2014;8(4):1461-1469.
      Species: Human
      Sample Type: Serum
    16. Endocannabinoids affect innate immunity of Muller glia during HIV-1 Tat cytotoxicity.
      Authors: Krishnan G, Chatterjee N
      Mol Cell Neurosci, 2014;59(0):10-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. Exogenous IFN-beta regulates the RANKL-c-Fos-IFN-beta signaling pathway in the collagen antibody-induced arthritis model.
      Authors: Zhao R, Chen N, Zhou X, Miao P, Hu C, Qian L, Yu Q, Zhang J, Nie H, Chen X, Li P, Xu R, Xiao L, Zhang X, Liu J, Zhang D
      J Transl Med, 2014;12(1):330.
      Species: Human
      Sample Type: Serum
    18. Neutrophil microparticles modulate cytokine production by natural killer cells.
      Authors: Pliyev B, Kalintseva M, Abdulaeva S, Yarygin K, Savchenko V
      Cytokine, 2013;65(2):126-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. N6-isopentenyladenosine, an endogenous isoprenoid end product, directly affects cytotoxic and regulatory functions of human NK cells through FDPS modulation.
      Authors: Ciaglia E, Pisanti S, Picardi P, Laezza C, Malfitano A, D'Alessandro A, Gazzerro P, Vitale M, Carbone E, Bifulco M
      J Leukoc Biol, 2013;94(6):1207-19.
      Species: Human
      Sample Type: Cell Culture Supernates
    20. Susceptibility-associated genetic variation at IL12B enhances Th1 polarization in psoriasis.
      Authors: Johnston A, Xing X, Swindell W, Kochkodan J, Riblett M, Nair R, Stuart P, Ding J, Voorhees J, Elder J, Gudjonsson J
      Hum Mol Genet, 2013;22(9):1807-15.
      Species: Human
      Sample Type: Serum
    21. Cleaved/associated TLR3 represents the primary form of the signaling receptor.
      Authors: Toscano F, Estornes Y, Virard F, Garcia-Cattaneo A, Pierrot A, Vanbervliet B, Bonnin M, Ciancanelli M, Zhang S, Funami K, Seya T, Matsumoto M, Pin J, Casanova J, Renno T, Lebecque S
      J Immunol, 2013;190(2):764-73.
      Species: Human
      Sample Type: Cell Culture Supernates
    22. Effects of interval and continuous exercise training on CD4 lymphocyte apoptotic and autophagic responses to hypoxic stress in sedentary men.
      Authors: Weng T, Huang S, Chuang Y, Wang J
      PLoS ONE, 2013;8(11):e80248.
      Species: Human
      Sample Type: Plasma
    23. Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor.
      Authors: Churchman SM, Geiler J, Parmar R, Horner EA, Church LD, Emery P, Buch MH, McDermott MF, Ponchel F
      Clin. Exp. Rheumatol., 2012;30(4):534-42.
      Species: Human
      Sample Type: Serum
    24. Analysis of the Interferon Gamma (rs2430561, +874T/A) Functional Gene Variant in Relation to the Presence of Cardiovascular Events in Rheumatoid Arthritis.
      Authors: Garcia-Bermudez M, Lopez-Mejias R, Gonzalez-Juanatey C, Corrales A, Robledo G, Castaneda S, Miranda-Filloy J, Blanco R, Fernandez-Gutierrez B, Balsa A, Gonzalez-Alvaro I, Gomez-Vaquero C, Llorca J, Martin J, Gonzalez-Gay M
      PLoS ONE, 2012;7(10):e47166.
      Species: Human
      Sample Type: Serum
    25. Potent adjuvanticity of a pure TLR7-agonistic imidazoquinoline dendrimer.
      Authors: Shukla N, Salunke D, Balakrishna R, Mutz C, Malladi S, David S
      PLoS ONE, 2012;7(8):e43612.
      Species: Human
      Sample Type: Cell Culture Supernates
    26. Engulfment of hematopoietic stem cells caused by down-regulation of CD47 is critical in the pathogenesis of hemophagocytic lymphohistiocytosis.
      Authors: Kuriyama T, Takenaka K, Kohno K, Yamauchi T, Daitoku S, Yoshimoto G, Kikushige Y, Kishimoto J, Abe Y, Harada N, Miyamoto T, Iwasaki H, Teshima T, Akashi K
      Blood, 2012;120(19):4058-67.
      Species: Human
      Sample Type: Serum
    27. Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system.
      Authors: Sun J, Fu F, Gu W, Yan R, Zhang G, Shen Z, Zhou Y, Wang H, Shen B, Zhang X
      PLoS ONE, 2011;6(9):e24751.
      Species: Human
      Sample Type: Cell Culture Supernates
    28. Cytokine Response Patterns in Severe Pandemic 2009 H1N1 and Seasonal Influenza among Hospitalized Adults.
      Authors: Lee N, Wong CK, Chan PK, Chan MC, Wong RY, Lun SW, Ngai KL, Lui GC, Wong BC, Lee SK, Choi KW, Hui DS
      PLoS ONE, 2011;6(10):e26050.
      Species: Human
      Sample Type: Plasma
    29. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
      Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
      Leukemia, 2011;25(1):161-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    30. Subnormal cytokine profile in the tear fluid of keratoconus patients.
      Authors: Jun AS, Cope L, Speck C, Feng X, Lee S, Meng H, Hamad A, Chakravarti S
      PLoS ONE, 2011;6(1):e16437.
      Species: Human
      Sample Type: Tears
    31. Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis.
      Authors: Mulder DJ, Pooni A, Mak N, Hurlbut DJ, Basta S, Justinich CJ
      Am. J. Pathol., 2011;178(2):744-53.
      Species: Human
      Sample Type: Whole Tissue
    32. Identification of human NK17/NK1 cells.
      Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
      PLoS ONE, 2011;6(10):e26780.
      Species: Human
      Sample Type: Cell Culture Supernates
    33. Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
      Authors: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H
      Arthritis Res. Ther., 2011;13(2):R52.
      Species: Human
      Sample Type: Serum
    34. Myeloid-derived suppressor cells in the peripheral blood of cancer patients contain a subset of immature neutrophils with impaired migratory properties.
      Authors: Brandau S, Trellakis S, Bruderek K, Schmaltz D, Steller G, Elian M, Suttmann H, Schenck M, Welling J, Zabel P, Lang S
      J. Leukoc. Biol., 2011;89(2):311-7.
      Species: Human
      Sample Type: Cell Culture Supernates
    35. Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus.
      Authors: Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J
      J. Invest. Dermatol., 2011;131(1):133-40.
      Species: Human
      Sample Type: Cell Culture Supernates
    36. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
      Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
      Diabetes, 2010;59(4):829-35.
      Species: Human
      Sample Type: Plasma
    37. B7-h3 augments the inflammatory response and is associated with human sepsis.
      Authors: Zhang G, Wang J, Kelly J
      J. Immunol., 2010;185(6):3677-84.
      Species: Human
      Sample Type: Plasma
    38. Interleukin-1beta, C-x-C motif ligand 10, and interferon-gamma serum levels in mixed cryoglobulinemia with or without autoimmune thyroiditis.
      Authors: Antonelli A, Ferri C, Ferrari SM, De Marco S, Di Domenicantonio A, Centanni M, Pupilli C, Villa E, Menichetti F, Fallahi P
      J. Interferon Cytokine Res., 2010;30(11):835-42.
      Species: Human
      Sample Type: Serum
    39. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
      Authors: Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
      J. Immunol., 2010;184(3):1642-52.
      Species: Human
      Sample Type: Serum
    40. A novel immunomodulatory mechanism of ribavirin in suppressing natural killer cell function.
      Authors: Ogbomo H, Michaelis M, Altenbrandt B, Doerr HW, Cinatl J
      Biochem. Pharmacol., 2010;79(2):188-97.
      Species: Human
      Sample Type: Cell Culture Supernates
    41. Shaping of iNKT cell repertoire after unrelated cord blood transplantation.
      Authors: Beziat V, Nguyen S, Exley M, Achour A, Simon T, Chevallier P, Sirvent A, Vigouroux S, Debre P, Rio B, Vieillard V, Lubrano-Berthelier C, Alatrakchi N
      Clin. Immunol., 2010;135(3):364-73.
      Species: Human
      Sample Type: Cell Culture Supernates
    42. Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
      Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
      J. Allergy Clin. Immunol., 2010;126(5):962.
      Species: Human
      Sample Type: Tissue Homogenates
    43. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
      Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
      J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
      Species: Human
      Sample Type: Cell Culture Supernates
    44. Arginase I-producing myeloid-derived suppressor cells in renal cell carcinoma are a subpopulation of activated granulocytes.
      Authors: Rodriguez PC, Ernstoff MS, Hernandez C, Atkins M, Zabaleta J, Sierra R, Ochoa AC
      Cancer Res., 2009;69(4):1553-60.
      Species: Human
      Sample Type: Plasma
    45. Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
      Authors: Mabilleau G, Sabokbar A
      PLoS ONE, 2009;4(1):e4173.
      Species: Human
      Sample Type: Cell Culture Supernates
    46. Cytokine secretion by human mesenchymal stem cells cocultured with damaged corneal epithelial cells.
      Authors: Oh JY, Kim MK, Shin MS, Wee WR, Lee JH
      Cytokine, 2009;46(1):100-3.
      Species: Human
      Sample Type: Cell Culture Supernates
    47. An evaluation of commercial fluorescent bead-based luminex cytokine assays.
      Authors: Djoba Siawaya JF, Roberts T, Babb C, Black G, Golakai HJ, Stanley K, Bapela NB, Hoal E, Parida S, van Helden P, Walzl G
      PLoS ONE, 2008;3(7):e2535.
      Species: Human
      Sample Type: Cell Culture Supernates
    48. Staphylococcus aureus in nasal lavage and biopsy of patients with chronic rhinosinusitis.
      Authors: Niederfuhr A, Kirsche H, Deutschle T, Poppert S, Riechelmann H, Wellinghausen N
      Allergy, 2008;63(10):1359-67.
      Species: Human
      Sample Type: Nasal Lavage
    49. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
      Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
      Eur. J. Immunol., 2008;38(4):1012-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    50. Synergistic interactions between interferon-gamma and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis.
      Authors: Stefanescu R, Bassett D, Modarresi R, Santiago F, Fakruddin M, Laurence J
      Blood, 2008;112(2):340-9.
      Species: Human
      Sample Type: Plasma
    51. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
      Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
      J. Immunol., 2008;181(11):8053-67.
      Species: Human
      Sample Type: BALF
    52. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
      Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
      Int. Immunol., 2008;20(10):1321-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    53. Panel of markers can accurately predict endometriosis in a subset of patients.
      Authors: Seeber B, Sammel MD, Fan X, Gerton GL, Shaunik A, Chittams J, Barnhart KT
      Fertil. Steril., 2007;89(5):1073-81.
      Species: Human
      Sample Type: Serum
    54. Translocation of bacterial DNA from Gram-positive microorganisms is associated with a species-specific inflammatory response in serum and ascitic fluid of patients with cirrhosis.
      Authors: Frances R, Gonzalez-Navajas JM, Zapater P, Munoz C, Cano R, Pascual S, Santana F, Marquez D, Perez-Mateo M, Such J
      Clin. Exp. Immunol., 2007;150(2):230-7.
      Species: Human
      Sample Type: Serum
    55. Interferon-beta and adhesion molecules (E-selectin and s-intracellular adhesion molecule-1) are detected in sera from patients with retinal vasculitis and are induced in retinal vascular endothelial cells by Toll-like receptor 3 signalling.
      Authors: Lee MT, Hooper LC, Kump L, Hayashi K, Nussenblatt R, Hooks JJ, Detrick B
      Clin. Exp. Immunol., 2007;147(1):71-80.
      Species: Human
      Sample Type: Serum
    56. 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
      Authors: Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR
      Cytokine, 2007;40(2):128-34.
      Species: Human
      Sample Type: Cell Culture Supernates
    57. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
      Authors: Lin YL, Liang YC, Chiang BL
      J. Leukoc. Biol., 2007;82(6):1473-80.
      Species: Human
      Sample Type: Cell Culture Supernates
    58. Fucoidan ingestion increases the expression of CXCR4 on human CD34+ cells.
      Authors: Irhimeh MR, Fitton JH, Lowenthal RM
      Exp. Hematol., 2007;35(6):989-94.
      Species: Human
      Sample Type: Plasma
    59. Phase 2 study of pegylated liposomal doxorubicin in combination with interleukin-12 for AIDS-related Kaposi sarcoma.
      Authors: Little RF, Aleman K, Kumar P, Wyvill KM, Pluda JM, Read-Connole E, Wang V, Pittaluga S, Catanzaro AT, Steinberg SM, Yarchoan R
      Blood, 2007;110(13):4165-71.
      Species: Human
      Sample Type: Serum
    60. Neuroinvasion of fluorescein-positive monocytes in acute simian immunodeficiency virus infection.
      Authors: Clay CC, Rodrigues DS, Ho YS, Fallert BA, Janatpour K, Reinhart TA, Esser U
      J. Virol., 2007;81(21):12040-8.
      Species: Primate - Macaca mulatta (Rhesus Macaque)
      Sample Type: Plasma
    61. IL-22 induces lipopolysaccharide-binding protein in hepatocytes: a potential systemic role of IL-22 in Crohn's disease.
      Authors: Wolk K, Witte E, Hoffmann U, Doecke WD, Endesfelder S, Asadullah K, Sterry W, Volk HD, Wittig BM, Sabat R
      J. Immunol., 2007;178(9):5973-81.
      Species: Human
      Sample Type: Cell Culture Supernates
    62. Impairment of human NK cell cytotoxic activity and cytokine release by cigarette smoke.
      Authors: Mian MF, Lauzon NM, Stampfli MR, Mossman KL, Ashkar AA
      J. Leukoc. Biol., 2007;83(3):774-84.
      Species: Human
      Sample Type: Cell Culture Supernates
    63. CD200 is induced by ERK and is a potential therapeutic target in melanoma.
      Authors: Petermann KB, Rozenberg GI, Zedek D, Groben P, McKinnon K, Buehler C, Kim WY, Shields JM, Penland S, Bear JE, Thomas NE, Serody JS, Sharpless NE
      J. Clin. Invest., 2007;117(12):3922-3929.
      Species: Human
      Sample Type: Cell Culture Supernates
    64. Human plasmacytoid predendritic cells activate NK cells through glucocorticoid-induced tumor necrosis factor receptor-ligand (GITRL).
      Authors: Hanabuchi S, Watanabe N, Wang YH, Ito T, Shaw J, Cao W, Qin FX, Liu YJ
      Blood, 2006;107(9):3617-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    65. The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
      Authors: Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N
      Blood, 2006;108(3):947-55.
      Species: Human
      Sample Type: Cell Culture Supernates
    66. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
      Authors: Ebert EC, Mehta V
      Cell. Immunol., 2006;241(1):7-13.
      Species: Human
      Sample Type: Cell Culture Supernates
    67. Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock.
      Authors: Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A
      Am. J. Respir. Crit. Care Med., 2006;174(1):51-7.
      Species: Human
      Sample Type: Plasma
    68. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
      Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
      Clin. Cancer Res., 2006;12(2):376-82.
      Species: Human
      Sample Type: Plasma
    69. Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma.
      Authors: Yoshikawa H, Sato K, Edahiro S, Furukawa Y, Maruta T, Iwasa K, Watanabe H, Takaoka S, Suzuki Y, Takamori M, Yamada M
      J. Neuroimmunol., 2006;175(1):169-75.
      Species: Human
      Sample Type: Serum
    70. Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.
      Authors: Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M
      Am. J. Respir. Crit. Care Med., 2006;173(12):1370-6.
      Species: Human
      Sample Type: Serum
    71. Over-expression of p53 mutants in LNCaP cells alters tumor growth and angiogenesis in vivo.
      Authors: Perryman LA, Blair JM, Kingsley EA, Szymanska B, Ow KT, Wen VW, MacKenzie KL, Vermeulen PB, Jackson P, Russell PJ
      Biochem. Biophys. Res. Commun., 2006;345(3):1207-14.
      Species: Human
      Sample Type: Cell Culture Supernates
    72. Th2 cytokine response in Major Depressive Disorder patients before treatment.
      Authors: Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G
      J. Neuroimmunol., 2006;172(1):156-65.
      Species: Human
      Sample Type: Serum
    73. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
      Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
      Gut, 2006;56(2):243-52.
      Species: Human
      Sample Type: Cell Culture Supernates
    74. IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis.
      Authors: Wolk K, Witte E, Wallace E, Docke WD, Kunz S, Asadullah K, Volk HD, Sterry W, Sabat R
      Eur. J. Immunol., 2006;36(5):1309-23.
      Species: Human
      Sample Type: Plasma
    75. A novel bispecific protein (ULBP2-BB4) targeting the NKG2D receptor on natural killer (NK) cells and CD138 activates NK cells and has potent antitumor activity against human multiple myeloma in vitro and in vivo.
      Authors: von Strandmann EP, Hansen HP, Reiners KS, Schnell R, Borchmann P, Merkert S, Simhadri VR, Draube A, Reiser M, Purr I, Hallek M, Engert A
      Blood, 2006;107(5):1955-62.
      Species: Human
      Sample Type: Cell Culture Supernates
    76. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
      Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
      Microbes Infect., 2005;7(3):427-36.
      Species: Human
      Sample Type: Serum
    77. Respiratory syncytial virus and other respiratory viruses during the first 3 months of life promote a local TH2-like response.
      Authors: Kristjansson S, Bjarnarson SP, Wennergren G, Palsdottir AH, Arnadottir T, Haraldsson A, Jonsdottir I
      J. Allergy Clin. Immunol., 2005;116(4):805-11.
      Species: Human
      Sample Type: Tissue Secretion
    78. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
      Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
      Microbes Infect., 2005;7(3):427-36.
      Species: Human
      Sample Type: Serum
    79. Detection of interleukin 10 in cerebrospinal fluid of patients with subacute sclerosing panencephalitis.
      Authors: Mistchenko AS, Fornari MC, Viegas M, Barrero PR, Diez RA
      J. Neurovirol., 2005;11(1):66-9.
      Species: Human
      Sample Type: CSF
    80. Effect of piceatannol in human monocyte-derived dendritic cells in vitro.
      Authors: Takei M, Umeyama A, Arihara S, Matsumoto H
      J Pharm Sci, 2005;94(5):974-82.
      Species: Human
      Sample Type: Cell Culture Supernates
    81. Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b.
      Authors: Jensen J, Krakauer M, Sellebjerg F
      Cytokine, 2005;29(1):24-30.
      Species: Human
      Sample Type: Plasma
    82. Production of interferon gamma in respiratory syncytial virus infection of humans is not associated with interleukins 12 and 18.
      Authors: Garofalo RP, Hintz KH, Hill V, Ogra PL, Welliver RC
      J. Med. Virol., 2004;73(2):289-94.
      Species: Human
      Sample Type: Tissue Secretion
    83. Escape from cardiomyocyte apoptosis by enterovirus persistence due to elevated soluble Fas-receptors.
      Authors: Alter P, Maisch B
      Z Kardiol, 2004;93(7):524-32.
      Species: Human
      Sample Type: Serum
    84. Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
      Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
      J. Androl., 2004;25(2):250-4.
      Species: Human
      Sample Type: Seminal Plasma
    85. A novel form of complete IL-12/IL-23 receptor beta1 deficiency with cell surface-expressed nonfunctional receptors.
      Authors: Fieschi C, Bosticardo M, de Beaucoudrey L, Boisson-Dupuis S, Feinberg J, Santos OF, Bustamante J, Levy J, Candotti F, Casanova JL
      Blood, 2004;104(7):2095-101.
      Species: Human
      Sample Type: Cell Culture Supernates
    86. Specific recognition and killing of glioblastoma multiforme by interleukin 13-zetakine redirected cytolytic T cells.
      Authors: Kahlon KS, Brown C, Cooper LJ, Raubitschek A, Forman SJ, Jensen MC
      Cancer Res., 2004;64(24):9160-6.
      Species: Human
      Sample Type: Cell Culture Supernates
    87. IL-22 increases the innate immunity of tissues.
      Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
      Immunity, 2004;21(2):241-54.
      Species: Human
      Sample Type: Cell Culture Supernates
    88. Cytokines evaluation in nasal lavage of allergic children after Bacillus clausii administration: a pilot study.
      Authors: Ciprandi G, Tosca MA, Milanese M, Caligo G, Ricca V
      Pediatr Allergy Immunol, 2004;15(2):148-51.
      Species: Human
      Sample Type: Nasal Lavage
    89. Effects of antiasthmatic agents on the functions of peripheral blood monocyte-derived dendritic cells from atopic patients.
      Authors: Saeki S, Matsuse H, Kondo Y, Machida I, Kawano T, Tomari S, Obase Y, Fukushima C, Kohno S
      J. Allergy Clin. Immunol., 2004;114(3):538-44.
      Species: Human
      Sample Type: Cell Culture Supernates
    90. IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction.
      Authors: Wu C, Sakorafas P, Miller R, McCarthy D, Scesney S, Dixon R, Ghayur T
      J. Immunol., 2003;170(11):5571-7.
      Species: Human
      Sample Type: Cell Culture Supernates
    91. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
      Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
      J. Leukoc. Biol., 2003;73(5):584-90.
      Species: Human
      Sample Type: Cell Culture Supernates
    92. In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment.
      Authors: Durali D, de Goer de Herve MG, Giron-Michel J, Azzarone B, Delfraissy JF, Taoufik Y
      Blood, 2003;102(12):4084-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    93. The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx.
      Authors: Chen CD, Yang JH, Lin KC, Chao KH, Ho HN, Yang YS
      Hum. Reprod., 2002;17(1):128-33.
      Species: Human
      Sample Type: Hydrosalpinx Fluid
    94. A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R.
      Authors: Parham C, Chirica M, Timans J, Vaisberg E, Travis M, Cheung J, Pflanz S, Zhang R, Singh KP, Vega F, To W, Wagner J, O&amp;amp;apos;Farrell AM, McClanahan T, Zurawski S, Hannum C, Gorman D, Rennick DM, Kastelein RA, de Waal Malefyt R, Moore KW
      J. Immunol., 2002;168(11):5699-708.
      Species: Human
      Sample Type: Cell Culture Supernates
    95. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
      Authors: D&amp;amp;apos;Ambrosio D
      Eur. J. Immunol., 2002;32(10):2934-43.
      Species: Human
      Sample Type: Cell Culture Supernates
    96. Cytokine profile in Behcet's disease patients. Relationship with disease activity.
      Authors: Hamzaoui K, Hamzaoui A, Guemira F, Bessioud M, Hamza M, Ayed K
      Scand. J. Rheumatol., 2002;31(4):205-10.
      Species: Human
      Sample Type: Serum
    97. Cytokine pattern in blister fluid and sera of patients with pemphigus.
      Authors: Baroni A, Perfetto B, Ruocco E, Greco R, Criscuolo D, Ruocco V
      Dermatology (Basel), 2002;205(2):116-21.
      Species: Human
      Sample Type: Blister Fluid
    98. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
      Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
      Int. J. Cancer, 2002;101(2):183-9.
      Species: Human
      Sample Type: Ascites Fluid
    99. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
      Authors: Briard D, Brouty-Boye D, Azzarone B, Jasmin C
      J. Immunol., 2002;168(9):4326-32.
      Species: Human
      Sample Type: Cell Culture Supernates
    100. Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells.
      Authors: Lazetic S, Leong SR, Chang JC, Ong R, Dawes G, Punnonen J
      J. Biol. Chem., 2002;277(41):38660-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    101. Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis: in situ, in vivo, and in vitro studies.
      Authors: Jotwani R, Palucka AK, Al-Quotub M, Nouri-Shirazi M, Kim J, Bell D, Banchereau J, Cutler CW
      J. Immunol., 2001;167(8):4693-700.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    102. Modulation of human immune response by Echinococcus granulosus antigen B and its possible role in evading host defenses.
      Authors: Rigano R, Profumo E, Bruschi F, Carulli G, Azzara A, Ioppolo S, Buttari B, Ortona E, Margutti P, Teggi A, Siracusano A
      Infect. Immun., 2001;69(1):288-96.
      Species: Human
      Sample Type: Cell Culture Supernates
    103. An analysis of acute changes in interleukin-6 levels after treatment of hepatitis C with consensus interferon.
      Authors: Cotler SJ, Reddy KR, McCone J, Wolfe DL, Liu A, Craft TR, Ferris MW, Conrad AJ, Albrecht J, Morrissey M, Ganger DR, Rosenblate H, Blatt LM, Jensen DM, Taylor MW
      J. Interferon Cytokine Res., 2001;21(12):1011-9.
      Species: Human
      Sample Type: Serum
    104. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
      Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
      J. Leukoc. Biol., 2001;70(4):617-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    105. Restoration of cytotoxic T lymphocyte function in malignant pleural effusion: interleukin-15 vs. interleukin-2.
      Authors: Chen YM, Ting CC, Peng JW, Yang WK, Yang KY, Tsai CM, Perng RP
      J. Interferon Cytokine Res., 2000;20(1):31-9.
      Species: Human
      Sample Type: Pleural Effusion
    106. Low levels of interferon-gamma in nasal fluid accompany raised levels of T-helper 2 cytokines in children with ongoing allergic rhinitis.
      Authors: Benson M, Strannegard IL, Wennergren G, Strannegard O
      Pediatr Allergy Immunol, 2000;11(1):20-8.
      Species: Human
      Sample Type: Nasal Lavage
    107. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
      Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
      J. Exp. Med., 2000;192(2):219-26.
      Species: Human
      Sample Type: Cell Culture Supernates
    108. Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity.
      Authors: Black, K P, Merrill, K W, Jackson, S, Katz, J
      Oral Microbiol Immunol, 2000;15(2):74-81.
      Species: Human
      Sample Type: Saliva
    109. Cytokine production in rhesus monkeys infected with Plasmodium coatneyi.
      Authors: Yang C, Xiao L, Tongren JE, Sullivan J, Lal AA, Collins WE
      Am. J. Trop. Med. Hyg., 1999;61(2):226-9.
      Species: Primate
      Sample Type: Serum
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