IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
Human IFN-gamma Quantikine ELISA Kit
R&D Systems | Catalog # DIF50
New Version Available:
DIF50C
NEW
This product has been discontinuation and replaced by catalog # DIF50C.
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL)
Sensitivity
8 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
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Product Summary for Human IFN-gamma Quantikine ELISA Kit
The Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure IFN-gamma levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring human IFN-gamma samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human IFN-gamma
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 79.2 | 214 | 466 | 78.7 | 206 | 457 |
| Standard Deviation | 3.7 | 5.5 | 13.0 | 6.1 | 13.1 | 17.0 |
| CV% | 4.7 | 2.6 | 2.8 | 7.8 | 6.4 | 3.7 |
Recovery for Human IFN-gamma Quantikine ELISA Kit
The recovery of IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 93-109 |
| EDTA Plasma (n=5) | 98 | 88-111 |
| Serum (n=5) | 102 | 91-118 |
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IFN-gamma in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IFN-gamma Quantikine ELISA Kit
Human IFN-gamma ELISA Standard Curve
Human IFN-gamma Standard Curve ELISA
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-gamma
Long Name
Interferon gamma
Alternate Names
IFG, IFI, IFNG, IFNgamma
Entrez Gene IDs
Gene Symbol
IFNG
Additional IFN-gamma Products
Product Documents for Human IFN-gamma Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IFN-gamma Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human IFN-gamma Quantikine ELISA Kit
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Customer Reviews for Human IFN-gamma Quantikine ELISA Kit (18)
4.8 out of 5
18 Customer Ratings
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Sample Tested: Human serumVerified Customer | Posted 09/26/2022
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Sample Tested: Blood monocytesVerified Customer | Posted 10/18/2020
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Sample Tested: Peripheral blood mononuclear cells (PBMCs)Verified Customer | Posted 07/13/2020Rested vs. Stimulated freshly isolated human PBMCs for IFNg secretion. Easy-to-follow protocol and always robust results, even if re-using plates/reagents.
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Sample Tested: Serum-free Cell Culture MediaVerified Customer | Posted 06/26/2020
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Sample Tested: Serum-free Cell Culture MediaVerified Customer | Posted 01/06/2020
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Sample Tested: Cell culture supernatantVerified Customer | Posted 12/08/2019
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Sample Tested: Jurkat human acute T cell leukemia cell lineVerified Customer | Posted 08/06/2019
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Sample Tested: BMECVerified Customer | Posted 07/11/2019
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Sample Tested: Jurkat human acute T cell leukemia cell lineVerified Customer | Posted 04/04/2019
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Sample Tested: MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell lineVerified Customer | Posted 03/21/2019
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Sample Tested: Cell culture supernatantVerified Customer | Posted 12/17/2018
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Sample Tested: EDTA PlasmaVerified Customer | Posted 10/07/2018
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Sample Tested: Immature dendritic cellsVerified Customer | Posted 08/27/2018
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Sample Tested: activated mouse CD8 T cellVerified Customer | Posted 05/13/2018
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Sample Tested: CHP-100 human neuroblastoma cell lineVerified Customer | Posted 04/19/2018
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Sample Tested: Serum-free Cell Culture SupernatesVerified Customer | Posted 06/26/2017
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Sample Tested: SerumVerified Customer | Posted 04/24/2017The Human IFN-gamma Quantikine ELISA kit is used for Diagnostic use.
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Sample Tested: Mesenchymal stem cellsVerified Customer | Posted 06/21/2016
There are no reviews that match your criteria.
Protocols
View specific protocols for Human IFN-gamma Quantikine ELISA Kit (DIF50):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
100 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
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- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Hematopoietic Stem Cell Differentiation Pathways & Lineage-specific Markers
IL-15 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
Innate Lymphoid Cell Differentiation Pathways
