Human IFN-gamma Quantikine ELISA Kit

  (111 citations)
(3 Reviews)
  
Product Details
Assay Procedure
Citations (111)
FAQs
Supplemental Products
Reviews
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL)
  • Sensitivity
    8 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant human IFN-gamma
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC01-1 , Quantikine Immunoassay Control Group 1 - Please Inquire
Product Summary
The Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure IFN-gamma levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring human IFN-gamma samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 79.2 214 466 78.7 206 457
Standard Deviation 3.7 5.5 13 6.1 13.1 17
CV% 4.7 2.6 2.8 7.8 6.4 3.7

Recovery

The recovery of IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 93-109
EDTA Plasma (n=5) 98 88-111
Serum (n=5) 102 91-118
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IFN-gamma in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IFN-gamma Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-gamma
IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
  • Long Name:
    Interferon gamma
  • Entrez Gene IDs:
    3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
  • Alternate Names:
    IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

111 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. The in vitro and ex vivo effect of Auranta 3001 in preventing Cryptosporidium hominis and Cryptosporidium parvum infection
    Authors: A Ch Stratak, F Sima, P Ward, M Linton, C Kelly, L Pinkerton, L Stef, I Pet, T Iancu, G Pircalabio, N Corcionivo
    Gut Pathog, 2017;9(0):49.
    Species: Bovine
    Sample Type: Cell Culture Supernates
  2. T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab
    Authors: U Bughani, A Saha, A Kuriakose, R Nair, RB Sadashivar, R Venkataram, S Patel, AT Deshchougu, SK S, E Montero, HV Pai, DV Palanivelu, R Melarkode, P Nair
    PLoS ONE, 2017;12(7):e0180088.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. Myeloid-derived suppressor cells are increased and correlated with type 2 immune responses, malnutrition, inflammation, and poor prognosis in patients with breast cancer
    Authors: K Gonda, M Shibata, T Ohtake, Y Matsumoto, K Tachibana, N Abe, H Ohto, K Sakurai, S Takenoshit
    Oncol Lett, 2017;14(2):1766-1774.
    Species: Human
    Sample Type: Cell Culture Supernates
  4. Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome
    Authors: KC Clark, FA Fierro, EM Ko, NJ Walker, B Arzi, CG Tepper, H Dahlenburg, A Cicchetto, A Kol, L Marsh, WJ Murphy, N Fazel, DL Borjesson
    Stem Cell Res Ther, 2017;8(1):69.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. Frequency and role of NKp46 and NKG2A in hepatitis B virus infection
    Authors: T Yoshioka, T Tatsumi, T Miyagi, K Mukai, K Nishio, A Nishio, Y Yokoyama, T Suda, T Kegasawa, M Shigekawa, H Hikita, R Sakamori, T Takehara
    PLoS ONE, 2017;12(3):e0174103.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
    J Immunol, 2016;197(5):1621-30.
    Species: Human
    Sample Type: Serum
  7. Alterations in the Fecal Microbiota of Patients with HIV-1 Infection: An Observational Study in A Chinese Population
    Sci Rep, 2016;6(0):30673.
    Species: Human
    Sample Type: Serum
  8. A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism.
    Authors: Mankad D, Dupuis A, Smile S, Roberts W, Brian J, Lui T, Genore L, Zaghloul D, Iaboni A, Marcon P, Anagnostou E
    Mol Autism, 2015;6(0):18.
    Species: Human
    Sample Type: Plasma
  9. WNT5A transforms intestinal CD8alphaalpha(+) IELs into an unconventional phenotype with pro-inflammatory features.
    Authors: Zhao D, Xu A, Dai Z, Peng J, Zhu M, Shen J, Zheng Q, Ran Z
    BMC Gastroenterol, 2015;15(0):173.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  10. CCL20-CCR6 Cytokine Network Facilitate Treg Activity in Advanced Grades and Metastatic Variants of Hepatocellular Carcinoma.
    Authors: Li W, Liu H
    Scand J Immunol, 2015;83(1):33-7.
    Species: Human
    Sample Type: Tissue Homogenates
  11. A Multidrug-resistant Engineered CAR T Cell for Allogeneic Combination Immunotherapy.
    Authors: Valton J, Guyot V, Marechal A, Filhol J, Juillerat A, Duclert A, Duchateau P, Poirot L
    Mol Ther, 2015;23(9):1507-18.
    Species: Human
    Sample Type: Cell Culture Supernates
  12. Comparative Multi-Donor Study of IFNgamma Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays.
    Authors: Hagen J, Zimmerman R, Goetz C, Bonnevier J, Houchins J, Reagan K, Kalyuzhny A
    Cells, 2015;4(1):84-95.
    Species: Human
    Sample Type: Whole Cells
  13. Exogenous IFN-beta regulates the RANKL-c-Fos-IFN-beta signaling pathway in the collagen antibody-induced arthritis model.
    Authors: Zhao R, Chen N, Zhou X, Miao P, Hu C, Qian L, Yu Q, Zhang J, Nie H, Chen X, Li P, Xu R, Xiao L, Zhang X, Liu J, Zhang D
    J Transl Med, 2014;12(1):330.
    Species: Human
    Sample Type: Serum
  14. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
    Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
    PLoS ONE, 2014;9(1):e86466.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy.
    Authors: Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J
    PLoS ONE, 2014;9(6):e99612.
    Species: Human
    Sample Type: Cell Culture Supernates
  16. Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma.
    Authors: Han L, Liu F, Li R, Li Z, Chen X, Zhou Z, Zhang X, Hu T, Zhang Y, Young K, Sun S, Wen J, Zhang M
    Oncol Lett, 2014;8(4):1461-1469.
    Species: Human
    Sample Type: Serum
  17. Live attenuated B. pertussis BPZE1 rescues the immune functions of Respiratory Syncytial virus infected human dendritic cells by promoting Th1/Th17 responses.
    Authors: Schiavoni I, Fedele G, Quattrini A, Bianco M, Schnoeller C, Openshaw P, Locht C, Ausiello C
    PLoS ONE, 2014;9(6):e100166.
    Species: Human
    Sample Type: Cell Culture Supernates
  18. EBV-encoded miR-BART20-5p and miR-BART8 inhibit the IFN-gamma-STAT1 pathway associated with disease progression in nasal NK-cell lymphoma.
    Authors: Huang W, Lin C
    Am J Pathol, 2014;184(4):1185-97.
    Species: Human
    Sample Type: Cell Culture Supernates
  19. Endocannabinoids affect innate immunity of Muller glia during HIV-1 Tat cytotoxicity.
    Authors: Krishnan G, Chatterjee N
    Mol Cell Neurosci, 2014;59(0):10-23.
    Species: Human
    Sample Type: Cell Culture Supernates
  20. Neutrophil microparticles modulate cytokine production by natural killer cells.
    Authors: Pliyev B, Kalintseva M, Abdulaeva S, Yarygin K, Savchenko V
    Cytokine, 2013;65(2):126-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  21. Cleaved/associated TLR3 represents the primary form of the signaling receptor.
    Authors: Toscano F, Estornes Y, Virard F, Garcia-Cattaneo A, Pierrot A, Vanbervliet B, Bonnin M, Ciancanelli M, Zhang S, Funami K, Seya T, Matsumoto M, Pin J, Casanova J, Renno T, Lebecque S
    J Immunol, 2013;190(2):764-73.
    Species: Human
    Sample Type: Cell Culture Supernates
  22. Effects of interval and continuous exercise training on CD4 lymphocyte apoptotic and autophagic responses to hypoxic stress in sedentary men.
    Authors: Weng T, Huang S, Chuang Y, Wang J
    PLoS ONE, 2013;8(11):e80248.
    Species: Human
    Sample Type: Plasma
  23. Susceptibility-associated genetic variation at IL12B enhances Th1 polarization in psoriasis.
    Authors: Johnston A, Xing X, Swindell W, Kochkodan J, Riblett M, Nair R, Stuart P, Ding J, Voorhees J, Elder J, Gudjonsson J
    Hum Mol Genet, 2013;22(9):1807-15.
    Species: Human
    Sample Type: Serum
  24. N6-isopentenyladenosine, an endogenous isoprenoid end product, directly affects cytotoxic and regulatory functions of human NK cells through FDPS modulation.
    Authors: Ciaglia E, Pisanti S, Picardi P, Laezza C, Malfitano A, D'Alessandro A, Gazzerro P, Vitale M, Carbone E, Bifulco M
    J Leukoc Biol, 2013;94(6):1207-19.
    Species: Human
    Sample Type: Cell Culture Supernates
  25. Engulfment of hematopoietic stem cells caused by down-regulation of CD47 is critical in the pathogenesis of hemophagocytic lymphohistiocytosis.
    Authors: Kuriyama T, Takenaka K, Kohno K, Yamauchi T, Daitoku S, Yoshimoto G, Kikushige Y, Kishimoto J, Abe Y, Harada N, Miyamoto T, Iwasaki H, Teshima T, Akashi K
    Blood, 2012;120(19):4058-67.
    Species: Human
    Sample Type: Serum
  26. Analysis of the Interferon Gamma (rs2430561, +874T/A) Functional Gene Variant in Relation to the Presence of Cardiovascular Events in Rheumatoid Arthritis.
    Authors: Garcia-Bermudez M, Lopez-Mejias R, Gonzalez-Juanatey C, Corrales A, Robledo G, Castaneda S, Miranda-Filloy J, Blanco R, Fernandez-Gutierrez B, Balsa A, Gonzalez-Alvaro I, Gomez-Vaquero C, Llorca J, Martin J, Gonzalez-Gay M
    PLoS ONE, 2012;7(10):e47166.
    Species: Human
    Sample Type: Serum
  27. Potent adjuvanticity of a pure TLR7-agonistic imidazoquinoline dendrimer.
    Authors: Shukla N, Salunke D, Balakrishna R, Mutz C, Malladi S, David S
    PLoS ONE, 2012;7(8):e43612.
    Species: Human
    Sample Type: Cell Culture Supernates
  28. Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor.
    Authors: Churchman SM, Geiler J, Parmar R, Horner EA, Church LD, Emery P, Buch MH, McDermott MF, Ponchel F
    Clin. Exp. Rheumatol., 2012;30(4):534-42.
    Species: Human
    Sample Type: Serum
  29. Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system.
    Authors: Sun J, Fu F, Gu W, Yan R, Zhang G, Shen Z, Zhou Y, Wang H, Shen B, Zhang X
    PLoS ONE, 2011;6(9):e24751.
    Species: Human
    Sample Type: Cell Culture Supernates
  30. Cytokine Response Patterns in Severe Pandemic 2009 H1N1 and Seasonal Influenza among Hospitalized Adults.
    Authors: Lee N, Wong CK, Chan PK, Chan MC, Wong RY, Lun SW, Ngai KL, Lui GC, Wong BC, Lee SK, Choi KW, Hui DS
    PLoS ONE, 2011;6(10):e26050.
    Species: Human
    Sample Type: Plasma
  31. Subnormal cytokine profile in the tear fluid of keratoconus patients.
    Authors: Jun AS, Cope L, Speck C, Feng X, Lee S, Meng H, Hamad A, Chakravarti S
    PLoS ONE, 2011;6(1):e16437.
    Species: Human
    Sample Type: Tears
  32. Myeloid-derived suppressor cells in the peripheral blood of cancer patients contain a subset of immature neutrophils with impaired migratory properties.
    Authors: Brandau S, Trellakis S, Bruderek K, Schmaltz D, Steller G, Elian M, Suttmann H, Schenck M, Welling J, Zabel P, Lang S
    J. Leukoc. Biol., 2011;89(2):311-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  33. Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus.
    Authors: Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J
    J. Invest. Dermatol., 2011;131(1):133-40.
    Species: Human
    Sample Type: Cell Culture Supernates
  34. Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis.
    Authors: Mulder DJ, Pooni A, Mak N, Hurlbut DJ, Basta S, Justinich CJ
    Am. J. Pathol., 2011;178(2):744-53.
    Species: Human
    Sample Type: Whole Tissue
  35. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011;6(10):e26780.
    Species: Human
    Sample Type: Cell Culture Supernates
  36. Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
    Authors: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H
    Arthritis Res. Ther., 2011;13(2):R52.
    Species: Human
    Sample Type: Serum
  37. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
    Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
    Leukemia, 2011;25(1):161-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  38. Shaping of iNKT cell repertoire after unrelated cord blood transplantation.
    Authors: Beziat V, Nguyen S, Exley M, Achour A, Simon T, Chevallier P, Sirvent A, Vigouroux S, Debre P, Rio B, Vieillard V, Lubrano-Berthelier C, Alatrakchi N
    Clin. Immunol., 2010;135(3):364-73.
    Species: Human
    Sample Type: Cell Culture Supernates
  39. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
    Authors: Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
    J. Immunol., 2010;184(3):1642-52.
    Species: Human
    Sample Type: Serum
  40. Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
    Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
    J. Allergy Clin. Immunol., 2010;126(5):962.
    Species: Human
    Sample Type: Tissue Homogenates
  41. A novel immunomodulatory mechanism of ribavirin in suppressing natural killer cell function.
    Authors: Ogbomo H, Michaelis M, Altenbrandt B, Doerr HW, Cinatl J
    Biochem. Pharmacol., 2010;79(2):188-97.
    Species: Human
    Sample Type: Cell Culture Supernates
  42. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
    Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
    Diabetes, 2010;59(4):829-35.
    Species: Human
    Sample Type: Plasma
  43. Interleukin-1beta, C-x-C motif ligand 10, and interferon-gamma serum levels in mixed cryoglobulinemia with or without autoimmune thyroiditis.
    Authors: Antonelli A, Ferri C, Ferrari SM, De Marco S, Di Domenicantonio A, Centanni M, Pupilli C, Villa E, Menichetti F, Fallahi P
    J. Interferon Cytokine Res., 2010;30(11):835-42.
    Species: Human
    Sample Type: Serum
  44. B7-h3 augments the inflammatory response and is associated with human sepsis.
    Authors: Zhang G, Wang J, Kelly J
    J. Immunol., 2010;185(6):3677-84.
    Species: Human
    Sample Type: Plasma
  45. Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
    Authors: Mabilleau G, Sabokbar A
    PLoS ONE, 2009;4(1):e4173.
    Species: Human
    Sample Type: Cell Culture Supernates
  46. Arginase I-producing myeloid-derived suppressor cells in renal cell carcinoma are a subpopulation of activated granulocytes.
    Authors: Rodriguez PC, Ernstoff MS, Hernandez C, Atkins M, Zabaleta J, Sierra R, Ochoa AC
    Cancer Res., 2009;69(4):1553-60.
    Species: Human
    Sample Type: Plasma
  47. Cytokine secretion by human mesenchymal stem cells cocultured with damaged corneal epithelial cells.
    Authors: Oh JY, Kim MK, Shin MS, Wee WR, Lee JH
    Cytokine, 2009;46(1):100-3.
    Species: Human
    Sample Type: Cell Culture Supernates
  48. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
    Species: Human
    Sample Type: Cell Culture Supernates
  49. An evaluation of commercial fluorescent bead-based luminex cytokine assays.
    Authors: Djoba Siawaya JF, Roberts T, Babb C, Black G, Golakai HJ, Stanley K, Bapela NB, Hoal E, Parida S, van Helden P, Walzl G
    PLoS ONE, 2008;3(7):e2535.
    Species: Human
    Sample Type: Cell Culture Supernates
  50. Synergistic interactions between interferon-gamma and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis.
    Authors: Stefanescu R, Bassett D, Modarresi R, Santiago F, Fakruddin M, Laurence J
    Blood, 2008;112(2):340-9.
    Species: Human
    Sample Type: Plasma
  51. Staphylococcus aureus in nasal lavage and biopsy of patients with chronic rhinosinusitis.
    Authors: Niederfuhr A, Kirsche H, Deutschle T, Poppert S, Riechelmann H, Wellinghausen N
    Allergy, 2008;63(10):1359-67.
    Species: Human
    Sample Type: Nasal Lavage
  52. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008;38(4):1012-23.
    Species: Human
    Sample Type: Cell Culture Supernates
  53. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Type: BALF
  54. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
    Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
    Int. Immunol., 2008;20(10):1321-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  55. CD200 is induced by ERK and is a potential therapeutic target in melanoma.
    Authors: Petermann KB, Rozenberg GI, Zedek D, Groben P, McKinnon K, Buehler C, Kim WY, Shields JM, Penland S, Bear JE, Thomas NE, Serody JS, Sharpless NE
    J. Clin. Invest., 2007;117(12):3922-3929.
    Species: Human
    Sample Type: Cell Culture Supernates
  56. Phase 2 study of pegylated liposomal doxorubicin in combination with interleukin-12 for AIDS-related Kaposi sarcoma.
    Authors: Little RF, Aleman K, Kumar P, Wyvill KM, Pluda JM, Read-Connole E, Wang V, Pittaluga S, Catanzaro AT, Steinberg SM, Yarchoan R
    Blood, 2007;110(13):4165-71.
    Species: Human
    Sample Type: Serum
  57. 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
    Authors: Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR
    Cytokine, 2007;40(2):128-34.
    Species: Human
    Sample Type: Cell Culture Supernates
  58. Interferon-beta and adhesion molecules (E-selectin and s-intracellular adhesion molecule-1) are detected in sera from patients with retinal vasculitis and are induced in retinal vascular endothelial cells by Toll-like receptor 3 signalling.
    Authors: Lee MT, Hooper LC, Kump L, Hayashi K, Nussenblatt R, Hooks JJ, Detrick B
    Clin. Exp. Immunol., 2007;147(1):71-80.
    Species: Human
    Sample Type: Serum
  59. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
    Authors: Lin YL, Liang YC, Chiang BL
    J. Leukoc. Biol., 2007;82(6):1473-80.
    Species: Human
    Sample Type: Cell Culture Supernates
  60. Fucoidan ingestion increases the expression of CXCR4 on human CD34+ cells.
    Authors: Irhimeh MR, Fitton JH, Lowenthal RM
    Exp. Hematol., 2007;35(6):989-94.
    Species: Human
    Sample Type: Plasma
  61. Neuroinvasion of fluorescein-positive monocytes in acute simian immunodeficiency virus infection.
    Authors: Clay CC, Rodrigues DS, Ho YS, Fallert BA, Janatpour K, Reinhart TA, Esser U
    J. Virol., 2007;81(21):12040-8.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Plasma
  62. Translocation of bacterial DNA from Gram-positive microorganisms is associated with a species-specific inflammatory response in serum and ascitic fluid of patients with cirrhosis.
    Authors: Frances R, Gonzalez-Navajas JM, Zapater P, Munoz C, Cano R, Pascual S, Santana F, Marquez D, Perez-Mateo M, Such J
    Clin. Exp. Immunol., 2007;150(2):230-7.
    Species: Human
    Sample Type: Serum
  63. Impairment of human NK cell cytotoxic activity and cytokine release by cigarette smoke.
    Authors: Mian MF, Lauzon NM, Stampfli MR, Mossman KL, Ashkar AA
    J. Leukoc. Biol., 2007;83(3):774-84.
    Species: Human
    Sample Type: Cell Culture Supernates
  64. Panel of markers can accurately predict endometriosis in a subset of patients.
    Authors: Seeber B, Sammel MD, Fan X, Gerton GL, Shaunik A, Chittams J, Barnhart KT
    Fertil. Steril., 2007;89(5):1073-81.
    Species: Human
    Sample Type: Serum
  65. IL-22 induces lipopolysaccharide-binding protein in hepatocytes: a potential systemic role of IL-22 in Crohn's disease.
    Authors: Wolk K, Witte E, Hoffmann U, Doecke WD, Endesfelder S, Asadullah K, Sterry W, Volk HD, Wittig BM, Sabat R
    J. Immunol., 2007;178(9):5973-81.
    Species: Human
    Sample Type: Cell Culture Supernates
  66. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
    Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
    Gut, 2006;56(2):243-52.
    Species: Human
    Sample Type: Cell Culture Supernates
  67. Human plasmacytoid predendritic cells activate NK cells through glucocorticoid-induced tumor necrosis factor receptor-ligand (GITRL).
    Authors: Hanabuchi S, Watanabe N, Wang YH, Ito T, Shaw J, Cao W, Qin FX, Liu YJ
    Blood, 2006;107(9):3617-23.
    Species: Human
    Sample Type: Cell Culture Supernates
  68. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Sample Type: Cell Culture Supernates
  69. Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock.
    Authors: Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A
    Am. J. Respir. Crit. Care Med., 2006;174(1):51-7.
    Species: Human
    Sample Type: Plasma
  70. The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
    Authors: Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N
    Blood, 2006;108(3):947-55.
    Species: Human
    Sample Type: Cell Culture Supernates
  71. A novel bispecific protein (ULBP2-BB4) targeting the NKG2D receptor on natural killer (NK) cells and CD138 activates NK cells and has potent antitumor activity against human multiple myeloma in vitro and in vivo.
    Authors: von Strandmann EP, Hansen HP, Reiners KS, Schnell R, Borchmann P, Merkert S, Simhadri VR, Draube A, Reiser M, Purr I, Hallek M, Engert A
    Blood, 2006;107(5):1955-62.
    Species: Human
    Sample Type: Cell Culture Supernates
  72. Over-expression of p53 mutants in LNCaP cells alters tumor growth and angiogenesis in vivo.
    Authors: Perryman LA, Blair JM, Kingsley EA, Szymanska B, Ow KT, Wen VW, MacKenzie KL, Vermeulen PB, Jackson P, Russell PJ
    Biochem. Biophys. Res. Commun., 2006;345(3):1207-14.
    Species: Human
    Sample Type: Cell Culture Supernates
  73. Th2 cytokine response in Major Depressive Disorder patients before treatment.
    Authors: Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G
    J. Neuroimmunol., 2006;172(1):156-65.
    Species: Human
    Sample Type: Serum
  74. Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.
    Authors: Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M
    Am. J. Respir. Crit. Care Med., 2006;173(12):1370-6.
    Species: Human
    Sample Type: Serum
  75. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
    Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
    Clin. Cancer Res., 2006;12(2):376-82.
    Species: Human
    Sample Type: Plasma
  76. Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma.
    Authors: Yoshikawa H, Sato K, Edahiro S, Furukawa Y, Maruta T, Iwasa K, Watanabe H, Takaoka S, Suzuki Y, Takamori M, Yamada M
    J. Neuroimmunol., 2006;175(1):169-75.
    Species: Human
    Sample Type: Serum
  77. IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis.
    Authors: Wolk K, Witte E, Wallace E, Docke WD, Kunz S, Asadullah K, Volk HD, Sterry W, Sabat R
    Eur. J. Immunol., 2006;36(5):1309-23.
    Species: Human
    Sample Type: Plasma
  78. Effect of piceatannol in human monocyte-derived dendritic cells in vitro.
    Authors: Takei M, Umeyama A, Arihara S, Matsumoto H
    J Pharm Sci, 2005;94(5):974-82.
    Species: Human
    Sample Type: Cell Culture Supernates
  79. Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b.
    Authors: Jensen J, Krakauer M, Sellebjerg F
    Cytokine, 2005;29(1):24-30.
    Species: Human
    Sample Type: Plasma
  80. Detection of interleukin 10 in cerebrospinal fluid of patients with subacute sclerosing panencephalitis.
    Authors: Mistchenko AS, Fornari MC, Viegas M, Barrero PR, Diez RA
    J. Neurovirol., 2005;11(1):66-9.
    Species: Human
    Sample Type: CSF
  81. Respiratory syncytial virus and other respiratory viruses during the first 3 months of life promote a local TH2-like response.
    Authors: Kristjansson S, Bjarnarson SP, Wennergren G, Palsdottir AH, Arnadottir T, Haraldsson A, Jonsdottir I
    J. Allergy Clin. Immunol., 2005;116(4):805-11.
    Species: Human
    Sample Type: Tissue Secretion
  82. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
    Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
    Microbes Infect., 2005;7(3):427-36.
    Species: Human
    Sample Type: Serum
  83. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
    Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
    Microbes Infect., 2005;7(3):427-36.
    Species: Human
    Sample Type: Serum
  84. Specific recognition and killing of glioblastoma multiforme by interleukin 13-zetakine redirected cytolytic T cells.
    Authors: Kahlon KS, Brown C, Cooper LJ, Raubitschek A, Forman SJ, Jensen MC
    Cancer Res., 2004;64(24):9160-6.
    Species: Human
    Sample Type: Cell Culture Supernates
  85. Escape from cardiomyocyte apoptosis by enterovirus persistence due to elevated soluble Fas-receptors.
    Authors: Alter P, Maisch B
    Z Kardiol, 2004;93(7):524-32.
    Species: Human
    Sample Type: Serum
  86. Effects of antiasthmatic agents on the functions of peripheral blood monocyte-derived dendritic cells from atopic patients.
    Authors: Saeki S, Matsuse H, Kondo Y, Machida I, Kawano T, Tomari S, Obase Y, Fukushima C, Kohno S
    J. Allergy Clin. Immunol., 2004;114(3):538-44.
    Species: Human
    Sample Type: Cell Culture Supernates
  87. IL-22 increases the innate immunity of tissues.
    Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
    Immunity, 2004;21(2):241-54.
    Species: Human
    Sample Type: Cell Culture Supernates
  88. Production of interferon gamma in respiratory syncytial virus infection of humans is not associated with interleukins 12 and 18.
    Authors: Garofalo RP, Hintz KH, Hill V, Ogra PL, Welliver RC
    J. Med. Virol., 2004;73(2):289-94.
    Species: Human
    Sample Type: Tissue Secretion
  89. Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
    Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
    J. Androl., 2004;25(2):250-4.
    Species: Human
    Sample Type: Seminal Plasma
  90. Cytokines evaluation in nasal lavage of allergic children after Bacillus clausii administration: a pilot study.
    Authors: Ciprandi G, Tosca MA, Milanese M, Caligo G, Ricca V
    Pediatr Allergy Immunol, 2004;15(2):148-51.
    Species: Human
    Sample Type: Nasal Lavage
  91. A novel form of complete IL-12/IL-23 receptor beta1 deficiency with cell surface-expressed nonfunctional receptors.
    Authors: Fieschi C, Bosticardo M, de Beaucoudrey L, Boisson-Dupuis S, Feinberg J, Santos OF, Bustamante J, Levy J, Candotti F, Casanova JL
    Blood, 2004;104(7):2095-101.
    Species: Human
    Sample Type: Cell Culture Supernates
  92. IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction.
    Authors: Wu C, Sakorafas P, Miller R, McCarthy D, Scesney S, Dixon R, Ghayur T
    J. Immunol., 2003;170(11):5571-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  93. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003;73(5):584-90.
    Species: Human
    Sample Type: Cell Culture Supernates
  94. In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment.
    Authors: Durali D, de Goer de Herve MG, Giron-Michel J, Azzarone B, Delfraissy JF, Taoufik Y
    Blood, 2003;102(12):4084-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  95. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
    Authors: Briard D, Brouty-Boye D, Azzarone B, Jasmin C
    J. Immunol., 2002;168(9):4326-32.
    Species: Human
    Sample Type: Cell Culture Supernates
  96. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
    Authors: D&amp;amp;apos;Ambrosio D
    Eur. J. Immunol., 2002;32(10):2934-43.
    Species: Human
    Sample Type: Cell Culture Supernates
  97. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
    Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
    Int. J. Cancer, 2002;101(2):183-9.
    Species: Human
    Sample Type: Ascites Fluid
  98. Cytokine pattern in blister fluid and sera of patients with pemphigus.
    Authors: Baroni A, Perfetto B, Ruocco E, Greco R, Criscuolo D, Ruocco V
    Dermatology (Basel), 2002;205(2):116-21.
    Species: Human
    Sample Type: Blister Fluid
  99. A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R.
    Authors: Parham C, Chirica M, Timans J, Vaisberg E, Travis M, Cheung J, Pflanz S, Zhang R, Singh KP, Vega F, To W, Wagner J, O&amp;amp;apos;Farrell AM, McClanahan T, Zurawski S, Hannum C, Gorman D, Rennick DM, Kastelein RA, de Waal Malefyt R, Moore KW
    J. Immunol., 2002;168(11):5699-708.
    Species: Human
    Sample Type: Cell Culture Supernates
  100. Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells.
    Authors: Lazetic S, Leong SR, Chang JC, Ong R, Dawes G, Punnonen J
    J. Biol. Chem., 2002;277(41):38660-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  101. The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx.
    Authors: Chen CD, Yang JH, Lin KC, Chao KH, Ho HN, Yang YS
    Hum. Reprod., 2002;17(1):128-33.
    Species: Human
    Sample Type: Hydrosalpinx Fluid
  102. Cytokine profile in Behcet's disease patients. Relationship with disease activity.
    Authors: Hamzaoui K, Hamzaoui A, Guemira F, Bessioud M, Hamza M, Ayed K
    Scand. J. Rheumatol., 2002;31(4):205-10.
    Species: Human
    Sample Type: Serum
  103. Modulation of human immune response by Echinococcus granulosus antigen B and its possible role in evading host defenses.
    Authors: Rigano R, Profumo E, Bruschi F, Carulli G, Azzara A, Ioppolo S, Buttari B, Ortona E, Margutti P, Teggi A, Siracusano A
    Infect. Immun., 2001;69(1):288-96.
    Species: Human
    Sample Type: Cell Culture Supernates
  104. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
    Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
    J. Leukoc. Biol., 2001;70(4):617-23.
    Species: Human
    Sample Type: Cell Culture Supernates
  105. An analysis of acute changes in interleukin-6 levels after treatment of hepatitis C with consensus interferon.
    Authors: Cotler SJ, Reddy KR, McCone J, Wolfe DL, Liu A, Craft TR, Ferris MW, Conrad AJ, Albrecht J, Morrissey M, Ganger DR, Rosenblate H, Blatt LM, Jensen DM, Taylor MW
    J. Interferon Cytokine Res., 2001;21(12):1011-9.
    Species: Human
    Sample Type: Serum
  106. Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis: in situ, in vivo, and in vitro studies.
    Authors: Jotwani R, Palucka AK, Al-Quotub M, Nouri-Shirazi M, Kim J, Bell D, Banchereau J, Cutler CW
    J. Immunol., 2001;167(8):4693-700.
    Species: Human
    Sample Type: Gingival Crevicular Fluid (GCF)
  107. Low levels of interferon-gamma in nasal fluid accompany raised levels of T-helper 2 cytokines in children with ongoing allergic rhinitis.
    Authors: Benson M, Strannegard IL, Wennergren G, Strannegard O
    Pediatr Allergy Immunol, 2000;11(1):20-8.
    Species: Human
    Sample Type: Nasal Lavage
  108. Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity.
    Authors: Black, K P, Merrill, K W, Jackson, S, Katz, J
    Oral Microbiol Immunol, 2000;15(2):74-81.
    Species: Human
    Sample Type: Saliva
  109. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
    J. Exp. Med., 2000;192(2):219-26.
    Species: Human
    Sample Type: Cell Culture Supernates
  110. Restoration of cytotoxic T lymphocyte function in malignant pleural effusion: interleukin-15 vs. interleukin-2.
    Authors: Chen YM, Ting CC, Peng JW, Yang WK, Yang KY, Tsai CM, Perng RP
    J. Interferon Cytokine Res., 2000;20(1):31-9.
    Species: Human
    Sample Type: Pleural Effusion
  111. Cytokine production in rhesus monkeys infected with Plasmodium coatneyi.
    Authors: Yang C, Xiao L, Tongren JE, Sullivan J, Lal AA, Collins WE
    Am. J. Trop. Med. Hyg., 1999;61(2):226-9.
    Species: Primate
    Sample Type: Serum
Expand to show all Citations

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Group 1

Ctrl QC01-1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Quantikine ELISA Wash Buffer 1

ELISA WA126 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 3 reviews)

Have you used Human IFN-gamma Quantikine ELISA Kit?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human IFN-gamma Quantikine ELISA Kit.

We have 3 reviews tested in 1 species: A. thaliana.

Filter by Ratings
Excellent
(3)
All Ratings
Filter by Applications
All Applications
Filter by Species
A. thaliana
(3)
All Species

Images Ratings Applications Species Reviewed By Date Details
Excellent
  A. thaliana Anonymous 06/26/2017
Show

Summary

Sample Tested Serum-free Cell Culture Supernates
Species A. thaliana
 Human IFN-gamma Quantikine ELISA Kit DIF50 Array DIF50
Enlarge
Excellent
  A. thaliana Anonymous 04/24/2017
Show
Image Details
 Human IFN-gamma Quantikine ELISA Kit DIF50
: Human IFN-gamma Quantikine ELISA Kit [DIF50].

Summary

Sample Tested Serum
Species A. thaliana

Other Experimental Details

Other Experimental Details The Human IFN-gamma Quantikine ELISA kit is used for Diagnostic use.
 Human IFN-gamma Quantikine ELISA Kit DIF50 Array DIF50
Enlarge
Excellent
  A. thaliana Anonymous 06/21/2016
Show
Image Details
 Human IFN-gamma Quantikine ELISA Kit DIF50
: Human IFN-gamma Quantikine ELISA Kit [DIF50].

Summary

Sample Tested Mesenchymal stem cells
Species A. thaliana

Please scroll the above reviews for more

Order Details

Contact Information

reload

Feedback

Customer Information

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

R&D Systems Guarantee