CENPA Antibody (5A7-2E11) - BSA Free

Novus Biologicals | Catalog # NBP2-12892

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5A7-2E11

Format

BSA Free
View all CENPA Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to a portion of human CENP-A

Localization

Nucleus, Chromosome, Centromere, Kinetochore

Marker

Centromeres Marker

Specificity

Detects approx 18kDa. Recognizes Human CENP-A.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CENPA Antibody (5A7-2E11) - BSA Free

Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892]

Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892]

Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892] - analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.
Immunocytochemistry/ Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892]

Immunocytochemistry/ Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892]

Immunocytochemistry/Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892] - Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Applications for CENPA Antibody (5A7-2E11) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of CENP-A Antibody was sufficient for detection of CENPA in 20 ug of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CENPA

1 ug/mL was sufficient for detection of CENPA in 20ug of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Alternate Names

Centromere autoantigen A, centromere protein A (17kD), centromere protein ACENP-Acentromere protein A, 17kDa, histone H3-like centromeric protein A

Gene Symbol

CENPA

UniProt

NP_001035891.1 (Human)

Additional CENPA Products

Product Documents for CENPA Antibody (5A7-2E11) - BSA Free

Certificate of Analysis

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Product Specific Notices for CENPA Antibody (5A7-2E11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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