CENTD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84542

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HCLEHKDDKLRNRPRKHRSFNCLEDTEPEAPLGQPKGHKGLKTLRKTEDRNSKATLDSDHKLPSRVIEELNVVLQRSRTLPKELQDEQI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CENTD1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CENTD1 Antibody [NBP1-84542]

Immunocytochemistry/ Immunofluorescence: CENTD1 Antibody [NBP1-84542]

Immunocytochemistry/Immunofluorescence: CENTD1 Antibody [NBP1-84542] - Staining of human cell line U-2 OS shows localization to actin filaments & focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CENTD1 Antibody [NBP1-84542]

Immunohistochemistry-Paraffin: CENTD1 Antibody [NBP1-84542]

Immunohistochemistry-Paraffin: CENTD1 Antibody [NBP1-84542] - Staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.
CENTD1 Antibody - BSA Free Western Blot: CENTD1 Antibody - BSA Free [NBP1-84542]

Western Blot: CENTD1 Antibody - BSA Free [NBP1-84542]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Applications for CENTD1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CENTD1

CENTD1 is encoded by this gene contains ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. This protein lacks the predicted catalytic arginine in the RHO-GAP domain and is therefore unlikely to have RHO-GAP activity. While the encoded protein does contain a sterile alpha motif (SAM) commonly found in some signaling molecules, the function of the protein has not been determined. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

Arf and Rho GAP adapter protein 2, ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2, centaurin, delta 1, Centaurin-delta-1, CENTD1arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2, Cnt-d1, FLJ13675, FLJ44916, KIAA0580centaurin-delta-1, PARX, Protein PARX

Gene Symbol

ARAP2

Additional CENTD1 Products

Product Documents for CENTD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CENTD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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