CHAF1B Antibody (7N0I2)

Name: CHAF1B Antibody (7N0I2)
Brand: Novus Biologicals
SKU: NBP3-16545
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16545

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7N0I2 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 459-559 of human CHAF1B (Q13112). PLPGPSEEKTLQPSSQNTKAHPSRRVTLNTLQAWSKTTPRRINLTPLKTDTPPSSVPTSVISTPSTEEIQSETPGDAQGSPPELKRPRLDENKGGTESLDP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHAF1B Antibody (7N0I2)

Western Blot: CHAF1B Antibody (7N0I2) [NBP3-16545]

Western Blot: CHAF1B Antibody (7N0I2) [NBP3-16545] - Western blot analysis of extracts of various cell lines, using CHAF1B Rabbit mAb (NBP3-16545) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry-Paraffin: CHAF1B Antibody (7N0I2) [NBP3-16545]

Immunohistochemistry-Paraffin: CHAF1B Antibody (7N0I2) [NBP3-16545] - Immunohistochemistry of paraffin-embedded human colon carcinoma using CHAF1B Rabbit mAb (NBP3-16545) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for CHAF1B Antibody (7N0I2)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHAF1B

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

CAF-1 antibodies are useful tools for cell division, DNA replication and DNA packaging.

Alternate Names

CAF1, CAF-1, CAF1ACAF-1 subunit B, CAF1P60CAF-I 60 kDa subunit, CAF-IP60, chromatin assembly factor 1 subunit B, chromatin assembly factor 1, subunit B (p60), Chromatin assembly factor I p60 subunit, human chromatin assembly factor-I p60 subunit, 10, MPHOSPH7CAF-I p60, MPP7M-phase phosphoprotein 7

Gene Symbol

CHAF1B

Additional CHAF1B Products

Product Documents for CHAF1B Antibody (7N0I2)

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Certificate of Analysis

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Product Specific Notices for CHAF1B Antibody (7N0I2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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