CHD2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32563

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CHD2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: QHWYKDHHYGDRRHMDAHRSGSYRPNNMSRKRPYDQYSSDRDHRGHRDYYDRHHHDSKRRRSDEFRPQNYHQQDFRRMS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHD2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CHD2 Antibody [NBP2-32563]

Immunocytochemistry/ Immunofluorescence: CHD2 Antibody [NBP2-32563]

Immunocytochemistry/Immunofluorescence: CHD2 Antibody [NBP2-32563] - Immunofluorescent staining of human cell line SiHa shows localization to nucleus & vesicles.
CHD2 Antibody - BSA Free

CHD2 Antibody [NBP2-32563] -

Analysis in human testis and skeletal muscle tissues using NBP2-32563 antibody. Corresponding CHD2 RNA-seq data are presented for the same tissues.
CHD2 Antibody - BSA Free

CHD2 Antibody [NBP2-32563] -

Staining of human Testis shows strong nuclear positivity in spermatogonia.
CHD2 Antibody - BSA Free

CHD2 Antibody [NBP2-32563] -

Staining of human Endometrium shows strong nuclear positivity in glandular cells.
CHD2 Antibody - BSA Free

CHD2 Antibody [NBP2-32563] -

Staining of human Cerebral cortex shows strong nuclear positivity in neurons and glial cells.
CHD2 Antibody - BSA Free

CHD2 Antibody [NBP2-32563] -

Staining of human Skeletal muscle shows very weak nuclear positivity in myocytes.

Applications for CHD2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHD2

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

ATP-dependent helicase CHD2, CHD-2, chromodomain helicase DNA binding protein 2, chromodomain-helicase-DNA-binding protein 2, DKFZp547I1315, DKFZp686E01200, DKFZp781D1727, EC 3.6.1, EC 3.6.4.12, FLJ38614

Gene Symbol

CHD2

Additional CHD2 Products

Product Documents for CHD2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CHD2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CHD2 Antibody - BSA Free

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Protocols

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FAQs

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