CHD4 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-57519

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation Sequencing

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CHD4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human chromodomain helicase DNA binding protein 4 (Mi2 autoantigen 218 kDa protein) using the numbering given in entry NP_001264.2 (GeneID 1108).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHD4 Antibody - BSA Free

Western Blot: CHD4 Antibody [NB100-57519]

Western Blot: CHD4 Antibody [NB100-57519]

Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: CHD4 Antibody [NB100-57519]

Western Blot: CHD4 Antibody [NB100-57519]

Western Blot: CHD4 Antibody [NB100-57519] - Detection of Human CHD4/Mi2 beta by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody NB100-57519 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: CHD4 Antibody [NB100-57519]

Immunoprecipitation: CHD4 Antibody [NB100-57519]

Immunoprecipitation: CHD4 Antibody [NB100-57519] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57519 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57520, NB100-57521), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). For blotting immunoprecipitated CHD4, NBP2-77358 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for CHD4 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2- 10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CHD4

CHD4/Mi2beta is a helicase component of the nucleosome remodeling and deacetylase (NuRD) complex that functions to remodel chromatin and repress transcription. Outside of the NuRD complex, CHD4/Mi2beta can function as an activator of transcription in association with p300 histone acetyltransferase. Alternate names for CHD4/Mi2beta include chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b, and DKFZp686E06161.

Alternate Names

ATP-dependent helicase CHD4, CHD-4, chromodomain helicase DNA binding protein 4, chromodomain-helicase-DNA-binding protein 4, DKFZp686E06161, EC 3.6.1, EC 3.6.4.12, Mi-2 autoantigen 218 kDa protein, Mi-2b, Mi2-BETA

Entrez Gene IDs

1108 (Human); 107932 (Mouse)

Gene Symbol

CHD4

UniProt

NP_001264.2 (Human)

Additional CHD4 Products

Product Documents for CHD4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CHD4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CHD4 Antibody - BSA Free

Customer Reviews for CHD4 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review CHD4 Antibody - BSA Free and earn rewards!

Have you used CHD4 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies