CHD4 Antibody

Novus Biologicals | Catalog # NB100-57520

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CHD4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 250 and 300 of human chromodomain helicase DNA binding protein 4 (Mi2 autoantigen 218 kDa protein) using the numbering given in entry NP_001264.2 (GeneID 1108).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHD4 Antibody

Western Blot: CHD4 Antibody [NB100-57520]

Western Blot: CHD4 Antibody [NB100-57520]

Western Blot: CHD4 Antibody [NB100-57520] - Detection of Human and Mouse CHD4/Mi2 beta on HeLa whole cell lysate using NB100-57520. CHD4/Mi2 beta was also IPed by rabbit anti-CHD4/Mi2 beta antibodies NB100-57519 and NB100-57521.

Applications for CHD4 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CHD4

CHD4/Mi2beta is a helicase component of the nucleosome remodeling and deacetylase (NuRD) complex that functions to remodel chromatin and repress transcription. Outside of the NuRD complex, CHD4/Mi2beta can function as an activator of transcription in association with p300 histone acetyltransferase. Alternate names for CHD4/Mi2beta include chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b, and DKFZp686E06161.

Alternate Names

ATP-dependent helicase CHD4, CHD-4, chromodomain helicase DNA binding protein 4, chromodomain-helicase-DNA-binding protein 4, DKFZp686E06161, EC 3.6.1, EC 3.6.4.12, Mi-2 autoantigen 218 kDa protein, Mi-2b, Mi2-BETA

Entrez Gene IDs

1108 (Human); 107932 (Mouse)

Gene Symbol

CHD4

UniProt

NP_001264.2 (Human)

Additional CHD4 Products

Product Documents for CHD4 Antibody

Certificate of Analysis

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Product Specific Notices for CHD4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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