CHIP/STUB1 Antibody (2E12) - Azide and BSA Free

Novus Biologicals | Catalog # H00010273-M01

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Lambda Clone # 2E12

Format

Azide and BSA Free
View all CHIP/STUB1 Primary Antibodies »

Product Specifications

Immunogen

STUB1 (NP_005852.2, 204 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGWVEDY

Specificity

Reacts with STIP1 homology and U-box containing protein 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Lambda

Scientific Data Images for CHIP/STUB1 Antibody (2E12) - Azide and BSA Free

Sandwich ELISA: CHIP/STUB1 Antibody (2E12) [H00010273-M01] - Detection limit for recombinant GST tagged STUB1 is 0.3 ng/ml as a capture antibody.

Applications for CHIP/STUB1 Antibody (2E12) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CHIP/STUB1

STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation (Min et al., 2008 [PubMed 18411298]).[supplied by OMIM]

Long Name

C-terminus of Hsc70 Interacting Protein

Alternate Names

HSPABP2, NY-CO-7, SDCCAG7, STUB1, UBOX1

Entrez Gene IDs

10273 (Human)

Gene Symbol

STUB1

UniProt

NP_005852 (Human)

Additional CHIP/STUB1 Products

Product Documents for CHIP/STUB1 Antibody (2E12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CHIP/STUB1 Antibody (2E12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CHIP/STUB1 Antibody (2E12) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review CHIP/STUB1 Antibody (2E12) - Azide and BSA Free and earn rewards!

Have you used CHIP/STUB1 Antibody (2E12) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for CHIP/STUB1 Antibody (2E12) - Azide and BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I am interested in a monoclonal antibody for Stub1 protein (CHIP). I need the antibody for a structural characterization, so it's very important for me to know the reactive sequence. It would be great if you can give me information about what is the reactive domain (TPR or U-box).

    A: You are right to identify CHIP Antibody (2E12) (H00010273-M01) - this is our only monoclonal antibody for STUB1/CHIP. The immunogen sequence for H00010273-M01 is amino acids 204-303 of the canonical sequence (isoform 1) as described in UniProt (http://www.uniprot.org/uniprot/Q9UNE7). According to this UniProt page (the 'Regions' section), this corresponds to the U-box domain, with some amino acids either side, but not overlapping with a TPR repeat.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies