CHIP/STUB1 Antibody

Novus Biologicals | Catalog # NB300-982

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CHIP/STUB1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DAFISENGWVEDY corresponding to C-Terminus according to NP_005852.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CHIP/STUB1 Antibody

Western Blot: CHIP/STUB1 Antibody [NB300-982]

Western Blot: CHIP/STUB1 Antibody [NB300-982]

Western Blot: CHIP Antibody [NB300-982] - (0.3ug/ml) Analysis of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for CHIP/STUB1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in human muscle lysates (calculated MW of 34.9 kDa band according to NP_005852.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHIP/STUB1

STUB1 modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It has E3 ubiquitin protein ligase activity and targets misfolded chaperone substrates towards proteasomal degradation. STUB1 mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation.

Long Name

C-terminus of Hsc70 Interacting Protein

Alternate Names

HSPABP2, NY-CO-7, SDCCAG7, STUB1, UBOX1

Entrez Gene IDs

10273 (Human); 56424 (Mouse)

Gene Symbol

STUB1

UniProt

NP_005852. (Human)

Additional CHIP/STUB1 Products

Product Documents for CHIP/STUB1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CHIP/STUB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CHIP/STUB1 Antibody

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FAQs for CHIP/STUB1 Antibody

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  • Q: In researching our products for safety information I ran across one of your products (Product number IMG3137) which I need clarification. According to our documentation, this product contains Tris-saline, 0.02% Sodium azide, 0.5% BSA and is a liquid. I am

    A: With regard to product IMG-3137 (now NB300-982), the molarity of the Tris-saline solution is as follows: 20mM Tris, 150mM NaCl

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