Choline Kinase beta Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00001120-B01P

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
View all Choline Kinase beta Primary Antibodies »

Product Specifications

Immunogen

CHKB (NP_005189.2, 1 a.a. - 395 a.a.) full-length human protein. MAAEATAVAGSGAVGGCLAKDGLQQSKCPDTTPKRRRASSLSRDAERRAYQWCREYLGGAWRRVQPEELRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVLLRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYIPSRPLKTQELREPVLSAAIATKMAQFHGMEMPFTKEPHWLFGTMERYLKQIQDLPPTGLPEMNLLEMYSLKDEMGNLRKLLESTPSPVVFCHNDIQEGNILLLSEPENADSLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLAEAKKGETLSQEEQRKLEEDLLVEVSRYALASHFFWGLWSILQASMSTIEFGYLDYAQSRFQFYFQQKGQLTSVHSSS

Specificity

Reacts with choline kinase beta.

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for Choline Kinase beta Antibody - Azide and BSA Free

Western Blot: Choline Kinase beta Antibody [H00001120-B01P]

Western Blot: Choline Kinase beta Antibody [H00001120-B01P]

Western Blot: Choline Kinase beta Antibody [H00001120-B01P] - Analysis of CHKB expression in transfected 293T cell line by CHKB polyclonal antibody. Lane 1: CHKB transfected lysate(43.45 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Choline Kinase beta Antibody [H00001120-B01P]

Immunocytochemistry/ Immunofluorescence: Choline Kinase beta Antibody [H00001120-B01P]

Immunocytochemistry/Immunofluorescence: Choline Kinase beta Antibody [H00001120-B01P] - Analysis of purified antibody to CHKB on HeLa cell. (antibody concentration 10 ug/ml)

Applications for Choline Kinase beta Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
This antibody is reactive against transfected lysate in WB, IF and as a detection antibody in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Choline Kinase beta

Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq]

Alternate Names

CHETK, CHKL, ChoKB, CK, CKEKB, EK, EKB, Ethanolamine Kinase beta, MDCMC

Entrez Gene IDs

1120 (Human)

Gene Symbol

CHKB

UniProt

NP_005189.2 (Human)

Additional Choline Kinase beta Products

Product Documents for Choline Kinase beta Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Choline Kinase beta Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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