HCG is a glycoprotein hormone secreted in pregnancy by embryonic trophoblast cells during the first 7 weeks of gestation. Production subsequently shifts to the placenta, where it continues until term. HCG is a heterodimer consisting of an alpha chain and a beta chain. The alpha chain is common to several other hormones, including lutenizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), while the beta chain is unique to HCG. Both alpha and beta chains contain a cysteine knot motif, also found in growth factors such as TGF-beta family members.
Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (2E6B3) - BSA Free
Novus Biologicals | Catalog # NBP2-52442
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2E6B3
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human Chorionic Gonadotropin alpha Chain (hCG alpha) (AA: 25-147) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images
Western Blot: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (2E6B3)BSA Free [NBP2-52442]
Western Blot: Chorionic Gonadotropin alpha Chain (HCG alpha) Antibody (2E6B3) [NBP2-52442] - 1: Western blot analysis using CGA mAb against human CGA (AA: 25-147) recombinant protein. (Expected MW is 39.4 kDa)Western Blot: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (2E6B3)BSA Free [NBP2-52442]
Western Blot: Chorionic Gonadotropin alpha Chain (HCG alpha) Antibody (2E6B3) [NBP2-52442] - Analysis using CGA mAb against HEK293 (1) and CGA (AA: 25-147)-hIgGFc transfected HEK293 (2) cell lysate.ELISA: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (2E6B3) - BSA Free [NBP2-52442]
ELISA: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (2E6B3) [NBP2-52442] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).Applications
Application
Recommended Usage
ELISA
1:10000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Chorionic Gonadotropin alpha Chain (HCG alpha)
Alternate Names
alpha HCG, CGA, FSHA, GPHa1, LHA, TSHA
Gene Symbol
CGA
Additional Chorionic Gonadotropin alpha Chain (HCG alpha) Products
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Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
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