Key Product Details

Species Reactivity

Human

Applications

Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 77F12

Format

BSA Free
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Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with native human HCG (whole molecule).

Specificity

This specifically recognizes HCG; it reacts mainly with the alpha-subunit. It is cross-reacting with the whole molecule of HCG, hLH, hTSH and hFSH. Cross-reactivity with free beta-subunit of HCG was not detected.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Sandwich ELISA

1:100-1:2000
Application Notes
Detection of HCG in immunoassays. Suitable for immunochromatography. Recommended pairs for sandwich immunoassay (capture-detection): NB100-73002 - NB100-73001.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Chorionic Gonadotropin alpha Chain (HCG alpha)

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family.

Alternate Names

alpha HCG, CGA, FSHA, GPHa1, LHA, TSHA

Entrez Gene IDs

1081 (Human)

Gene Symbol

CGA

Additional Chorionic Gonadotropin alpha Chain (HCG alpha) Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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