Chorionic Gonadotropin beta Chain (hCG beta) Antibody (JE45-04)

Novus Biologicals | Catalog # NBP3-33129

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE45-04 expressed in HEK293
View all Chorionic Gonadotropin beta Chain (HCG beta) Primary Antibodies »

Product Specifications

Immunogen

Full length protein corresponding to Human Chorionic Gonadotropin beta Chain (hCG beta). (Uniprot: P0DN86)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Chorionic Gonadotropin beta Chain (hCG beta) Antibody (JE45-04) (NBP3-33129) is a recombinant monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Chorionic Gonadotropin beta Chain (hCG beta) Antibody (JE45-04)

Chorionic Gonadotropin beta Chain (hCG beta) Antibody (JE45-04)

Immunohistochemistry: Chorionic Gonadotropin beta Chain (hCG beta) Antibody (JE45-04) [NBP3-33129]

Immunohistochemistry: Chorionic Gonadotropin beta Chain (hCG beta) Antibody (JE45-04) [NBP3-33129] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Chorionic Gonadotropin beta Chain (hCG beta) antibody (NBP3-33129) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33129) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for Chorionic Gonadotropin beta Chain (hCG beta) Antibody (JE45-04)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chorionic Gonadotropin beta Chain (HCG beta)

Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.

Long Name

Chorionic Gonadotropin beta Chain

Alternate Names

CG-Beta, CGB, CGB3, CGB5, CGB8, Choriogonadotropin Subunit Beta

Gene Symbol

CGB3

Additional Chorionic Gonadotropin beta Chain (HCG beta) Products

Product Documents for Chorionic Gonadotropin beta Chain (hCG beta) Antibody (JE45-04)

Certificate of Analysis

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Product Specific Notices for Chorionic Gonadotropin beta Chain (hCG beta) Antibody (JE45-04)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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