CHREBP Antibody - BSA Free
Novus Biologicals | Catalog # NB400-136
![Immunocytochemistry/ Immunofluorescence: CHREBP Antibody [NB400-136]](https://resources.rndsystems.com/images/products/CHREBP-Antibody-Immunocytochemistry-Immunofluorescence-NB400-136-img0002.jpg "Immunocytochemistry/ Immunofluorescence: CHREBP Antibody [NB400-136]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
An internal synthetic peptide made to the human CHREBP protein sequence (between residues 50-150). [UniProt# Q9NP71, Isoform 1/Alpha]
Reactivity Notes
Human and Mouse. Immunogen sequence has 87% identity with rat.
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CHREBP Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: CHREBP Antibody [NB400-136]
Immunocytochemistry/Immunofluorescence: CHREBP Antibody [NB400-136] - Staining of a human hepatocyte using NB400-136. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).Applications for CHREBP Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Application Notes
This CHREBP antibody is useful for Immunocytochemistry/Immunofluorescence.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CHREBP
Alternate Names
BHLHD14, CHREBPcarbohydrate-responsive element-binding protein, Class D basic helix-loop-helix protein 14, MIOWS basic-helix-loop-helix leucine zipper protein, MLX interacting protein-like, MLX interactor, MLX-interacting protein-like, MONDOBWilliams-Beuren syndrome chromosomal region 14 protein, WBSCR14Williams-Beuren syndrome chromosome region 14 protein 1, Williams Beuren syndrome chromosome region 14, Williams-Beuren syndrome chromosome region 14 protein 2, WS-bHLHbHLHd14carbohydrate response element binding protein
Gene Symbol
MLXIPL
Additional CHREBP Products
Product Documents for CHREBP Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CHREBP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for CHREBP Antibody - BSA Free
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Protocols
View specific protocols for CHREBP Antibody - BSA Free (NB400-136):
CHREBP Antibody:
Procedure for immunofluorescent staining
1. Wash human hepatocytes grown on cover-slips with PBS twice.
2. Fix cells with 10% buffered formalin for 25 minutes at room temperature.
3. Wash cells with PBS 3 times.
4. Incubate with 0.5% Triton X-100 in PBS for 5 minutes.
5. Wash cells with PBS 3 times.
6. Block with 1% BSA in PBS for 15 minutes at 37C.
7. Incubate with rabbit anti-ChREBP antibody (NB 400-136) 1:100-1:500 in PBS with 1% BSA for 30 minutes at 37C.
8. Wash cells with PBS 3 times.
9. Incubate with Goat anti-rabbit Alexa Fluor 568 antibody 1:2,000 in PBS with 1% BSA for 30 minutes at 37C.
10. Wash cells with PBS 3 times.
11. Mount by Vectashield mounting medium with DAPI.
12. Take pictures by using Rhodamine and DAPI filters.
Procedure for immunofluorescent staining
1. Wash human hepatocytes grown on cover-slips with PBS twice.
2. Fix cells with 10% buffered formalin for 25 minutes at room temperature.
3. Wash cells with PBS 3 times.
4. Incubate with 0.5% Triton X-100 in PBS for 5 minutes.
5. Wash cells with PBS 3 times.
6. Block with 1% BSA in PBS for 15 minutes at 37C.
7. Incubate with rabbit anti-ChREBP antibody (NB 400-136) 1:100-1:500 in PBS with 1% BSA for 30 minutes at 37C.
8. Wash cells with PBS 3 times.
9. Incubate with Goat anti-rabbit Alexa Fluor 568 antibody 1:2,000 in PBS with 1% BSA for 30 minutes at 37C.
10. Wash cells with PBS 3 times.
11. Mount by Vectashield mounting medium with DAPI.
12. Take pictures by using Rhodamine and DAPI filters.
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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