Chromogranin A Antibody (CGA/414)

Novus Biologicals | Catalog # NBP2-29428

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat (Negative)

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CGA/414
View all Chromogranin A Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Chromogranin A protein (Uniprot: P10645)

Reactivity Notes

Does not react with Rat.

Mouse reactivity reported in scientific literature (PMID: 29352046).

Localization

Finely granular cytoplasmic

Marker

Neuroendocrine Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33198)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Chromogranin A Antibody (CGA/414)

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428] - Formalin-fixed, paraffin-embedded pancreas stained with Chromogranin A Ab (CGA414).
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428] - Formalin-fixed, paraffin-embedded human small cell Lung Carcinoma stained with Chromogranin A Monoclonal Antibody (CGA/414)
Chromogranin A Antibody (CGA/414)

Immunohistochemistry: Chromogranin A Antibody (CGA/414) [NBP2-29428] -

Expression of sst5TMD4 and co-localization with angiogenic marker in GEP-NET(A) Analysis of expression of angiogenic molecules and sst5TMD4 by specific serial immunohistochemistry in a pancreatic NET. Original magnification ×100 and ×400 (insets). N: normal tissue; T: tumor tissue. For specific immunostaining techniques see the “Materials and methods" section. (B) Expression of sst5TMD4 and angiogenic molecules by triple immunofluorescence in a gastrointestinal NET sample. Original magnification ×400. N: normal tissue; T: tumor tissue. For specific immunofluorescence techniques see the “Materials and methods" section. Scale bar for 100 μm is represented with a line for each Figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26673010), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Chromogranin A Antibody (CGA/414)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Chromogranin A

Chromogranin A is found in secretory vesicles of neurons and endocrine cells. It is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

UniProt

P10645

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody (CGA/414)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Chromogranin A Antibody (CGA/414)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Chromogranin A Antibody (CGA/414)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Chromogranin A Antibody (CGA/414)

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  • Q: I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?

    A:

    When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

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