CISH/CIS-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38474

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CIS-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: APSLELPKPVMQPLPAGAFLEEVAEGTPAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVRIEYADSSFRLDSNCLSR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CISH/CIS-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CISH/CIS-1 Antibody [NBP2-38474]

Immunocytochemistry/ Immunofluorescence: CISH/CIS-1 Antibody [NBP2-38474]

Immunocytochemistry/Immunofluorescence: CISH/CIS-1 Antibody [NBP2-38474] - Staining of human cell line U-2 OS shows localization to microtubules & cytokinetic bridge. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CISH/CIS-1 Antibody [NBP2-38474]

Immunohistochemistry-Paraffin: CISH/CIS-1 Antibody [NBP2-38474]

Immunohistochemistry-Paraffin: CISH/CIS-1 Antibody [NBP2-38474] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules and cells in glomeruli.

Applications for CISH/CIS-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CIS-1

CISH is encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor.

Long Name

Cytokine Inducible SH2-containing Protein

Alternate Names

CISH

Gene Symbol

CISH

UniProt

Q9NSE2

Additional CIS-1 Products

Product Documents for CISH/CIS-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CISH/CIS-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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