CKIP-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76238

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Avian - Chicken

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CKIP-1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human CKIP1. The immunogen is located within the last 50 amino acids of CKIP1.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CKIP-1 Antibody - BSA Free

Western Blot: CKIP-1 AntibodyBSA Free [NBP1-76238]

Western Blot: CKIP-1 AntibodyBSA Free [NBP1-76238]

Western Blot: CKIP-1 Antibody [NBP1-76238] - Human lung tissue lysate with CKIP1 antibody at (A) 1 and (B) 2 ug/mL.
CKIP-1 Antibody - BSA Free

Western Blot: CKIP-1 Antibody - BSA Free [NBP1-76238] -

Western Blot: CKIP-1 Antibody - BSA Free [NBP1-76238] - CKIP-1 directly interacts with Smurf1 to promote Smurf1 degradation.DF-1 chicken fibroblasts were transfected with the indicated constructs & then immunostained using anti-FLAG & anti-V5 antibodies. (A) Localization of FLAG-tagged CKIP-1 & V5-tagged Smurf1 alone. (B) Schematics for CKIP-1 FLAG constructs highlighting the membrane-binding PH domain (red) & Smurf1-binding LZ (blue) followed by a C-terminal FLAG tag (yellow). (C) Full-length CKIP-1 FLAG was cotransfected with full-length V5-Smurf1 or V5-Smurf1 C700A dominant-negative mutant. In addition, CKIP-1 FLAG truncation mutants were cotransfected with full-length V5-Smurf1, showing that the PH domain is required for efficient membrane localization, & the LZ domain is required for Smurf1 colocalization. Arrows indicate membrane localization. Scale bars represent 10 μm. (D) Chick embryos were electroporated at HH4 with V5-Smurf1 & CKIP-1 FLAG & then stained for V5 or FLAG at HH8 & imaged in cryosection. Scale bars represent 25 μm. (E) Western blot analysis of HH7 whole-embryo lysates electroporated with constructs indicated & probed for V5, pSmad1/5/8, & RFP. Bar graphs represent normalized V5 & pSmad1/5/8 staining intensity means ± SEM from 3 replicates. Underlying data can be found in S1 Data. P values from two-tailed Student t test. CKIP-1, casein kinase interacting protein 1; HH, Hamburger-Hamilton stage; LZ, leucine zipper motif; NC; neural crest domain; NS, not significant; PH, pleckstrin homology; pSmad1/5/8, phospho-Smads 1/5/8; RFP, red fluorescent protein; Smurf1, Smad ubiquitin regulatory factor 1. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29949573), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CKIP-1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CKIP-1

CKIP1 was identified through a yeast-two hybrid screening as a protein that would interact with only one of the two catalytic subunits of the casein kinase 2 complex. CKIP1 is a pleckstrin homology domain-containing protein localized within the nucleus and at the plasma membrane that interacts with CK2alpha but not CK2alpha', and is thought to play a role in targeting CK2alpha to a particular cellular location. CKIP1 has been implicated in muscle differentiation and the regulation of cell morphology and actin cytoskeleton. CKIP1 can also interact with other proteins such as ATM, an upstream kinase of p53, and recruit the nuclear ATM to the plasma membrane, suggesting CKIP1 may regulate ATM function through re-localizing ATM. CKIP1 can also form a complex with the kinase AKT, leading to a down-regulation of PI3K/AKT signaling and suppression of tumor growth in vivo.

Alternate Names

2810052M02Rik, Casein kinase 2-interacting protein 1, C-Jun-binding protein, CK2 interacting protein 1; HQ0024c protein, CK2-interacting protein 1, CKIP1, CKIP-1PH domain-containing family O member 1, JBP, OC120, Osteoclast maturation-associated gene 120 protein, pleckstrin homology domain containing, family O member 1, pleckstrin homology domain-containing family O member 1

Gene Symbol

PLEKHO1

UniProt

NP_057358 (Human)

Additional CKIP-1 Products

Product Documents for CKIP-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CKIP-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CKIP-1 Antibody - BSA Free

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