Claudin-12 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87450

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLPSPFWQPLYSHPPSMHTYSQPYSARSRLSAIEIDIPVVSHTT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Claudin-12 Antibody - BSA Free

Western Blot: Claudin-12 Antibody [NBP1-87450]

Western Blot: Claudin-12 Antibody [NBP1-87450]

Claudin-12-Antibody-Western-Blot-NBP1-87450-img0008.jpg
Immunohistochemistry-Paraffin: Claudin-12 Antibody [NBP1-87450]

Immunohistochemistry-Paraffin: Claudin-12 Antibody [NBP1-87450]

Immunohistochemistry-Paraffin: Claudin-12 Antibody [NBP1-87450] - Staining of human pancreas shows moderate cytoplasmic and membranous positivity in exocrine glandular cells along with moderate cytoplasmic staining in islets of Langerhans
Western Blot: Claudin-12 Antibody [NBP1-87450]

Western Blot: Claudin-12 Antibody [NBP1-87450]

Western Blot: Claudin-12 Antibody [NBP1-87450] - Analysis in human liver tissue.
Claudin-12 Antibody

Western Blot: Claudin-12 Antibody [NBP1-87450] -

Western Blot: Claudin-12 Antibody [NBP1-87450] - Increased CLDN-5 expression in the cerebellum of ASD subjects. a Western blots of brain lysates immunoblotted with anti-claudin-5, anti-claudin-12, SMA or actin antibody. b Densitometry analysis of the results from the western blots is shown, where the data are normalized against SMA expression & are expressed as the mean ± standard error. Quantitative results represent the average of three independent experiments. ***p < 0.001 Image collected & cropped by CiteAb from the following publication (http://molecularautism.biomedcentral.com/articles/10.1186/s13229-016-01…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Claudin-12 Antibody

Western Blot: Claudin-12 Antibody [NBP1-87450] -

Western Blot: Claudin-12 Antibody [NBP1-87450] - Increased CLDN-5 & decreased CLDN-12 expression in the cortex of ASD subjects. a Brain tissues were lysed & immunoblotted with anti-claudin-5, anti-claudin-12, SMA or actin antibody. b Densitometry analysis of the results from the western blots is shown, where the data are normalized against SMA expression & are expressed as the mean ± standard error. Quantitative results represent the average of three independent experiments.*p < 0.05; ****p < 0.0001 Image collected & cropped by CiteAb from the following publication (http://molecularautism.biomedcentral.com/articles/10.1186/s13229-016-01…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Claudin-12 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 29986305). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Claudin-12

Claudins, such as CLDN12, are components of epithelial cell tight junctions. Tight junctions regulate movement ofsolutes and ions through the paracellular space and prevent mixing of proteins and lipids in the outer leaflet of theapical and basolateral plasma membrane domains (Acharya et al., 2004).(supplied by OMIM)

Alternate Names

Claudin12, CLDN12

Gene Symbol

CLDN12

Additional Claudin-12 Products

Product Documents for Claudin-12 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Claudin-12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Claudin-12 Antibody - BSA Free

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Protocols

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