Claudin-3 Antibody (HL1528) - Azide and BSA Free

Name: Claudin-3 Antibody (HL1528) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-25413
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-25413

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (90%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1528 expressed in HEK293

Format

Azide and BSA Free

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Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Claudin-3. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Claudin-3 Antibody (HL1528) - Azide and BSA Free

Western Blot: Claudin-3 Antibody (HL1528) - Azide and BSA Free [NBP3-25413] -

Western Blot: Claudin-3 Antibody (HL1528) - Azide and BSA Free [NBP3-25413] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 3 antibody [HL1528] (NBP3-25413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Claudin-3 Antibody (HL1528) - Azide and BSA Free [NBP3-25413] -

Immunocytochemistry/Immunofluorescence: Claudin-3 Antibody (HL1528) - Azide and BSA Free [NBP3-25413] - Claudin 3 antibody [HL1528] detects Claudin 3 protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Claudin 3 stained by Claudin 3 antibody [HL1528] (NBP3-25413) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI.

Applications for Claudin-3 Antibody (HL1528) - Azide and BSA Free

Application

Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Claudin-3

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Alternate Names

Claudin3, CLDN3, CPE-R 2, CPETR2, HRVP1

Gene Symbol

CLDN3

Additional Claudin-3 Products

Product Documents for Claudin-3 Antibody (HL1528) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for Claudin-3 Antibody (HL1528) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.