CLPTM1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-32114
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 619 to 669 of human Cleft Lip and Palate Associated Transmembrane Protein 1 using the numbering given in entry NP_001285.1 (GeneID 1209).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CLPTM1 Antibody - BSA Free
Western Blot: CLPTM1 Antibody [NBP2-32114]
Western Blot: CLPTM1 Antibody [NBP2-32114] - Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CLPTM1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Western Blot: CLPTM1 Antibody [NBP2-32114]
Western Blot: CLPTM1 Antibody [NBP2-32114] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-CLPTM1 antibody NBP2-32114) used for IP at 6 ug per reaction. CLPTM1 was also immunoprecipitated by rabbit anti-CLPTM1 antibody BL16525. For blotting immunoprecipitated CLPTM1, NBP2-32114was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for CLPTM1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CLPTM1
Alternate Names
cleft lip and palate associated transmembrane protein 1, cleft lip and palate transmembrane protein 1
Gene Symbol
CLPTM1
UniProt
Additional CLPTM1 Products
Product Documents for CLPTM1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CLPTM1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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