Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI5A12
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Product Specifications
Immunogen
Full length human recombinant protein of human CNOT2(NP_055330) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CNOT2 Antibody (OTI5A12)
![Western Blot: CNOT2 Antibody (OTI5A12) [NBP2-46323]](https://resources.rndsystems.com/images/products/CNOT2-Antibody-5A12-Western-Blot-NBP2-46323-img0005.jpg "Western Blot: CNOT2 Antibody (OTI5A12) [NBP2-46323]")
Western Blot: CNOT2 Antibody (OTI5A12) [NBP2-46323]
Western Blot: CNOT2 Antibody (5A12) [NBP2-46323] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT2.![Immunohistochemistry: CNOT2 Antibody (OTI5A12) [NBP2-46323]](https://resources.rndsystems.com/images/products/CNOT2-Antibody-5A12-Immunohistochemistry-NBP2-46323-img0004.jpg "Immunohistochemistry: CNOT2 Antibody (OTI5A12) [NBP2-46323]")
Immunohistochemistry: CNOT2 Antibody (OTI5A12) [NBP2-46323]
Immunohistochemistry: CNOT2 Antibody (5A12) [NBP2-46323] - Analysis of Human tonsil tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: CNOT2 Antibody (OTI5A12) [NBP2-46323]](https://resources.rndsystems.com/images/products/CNOT2-Antibody-5A12-Immunohistochemistry-NBP2-46323-img0001.jpg "Immunohistochemistry: CNOT2 Antibody (OTI5A12) [NBP2-46323]")
Immunohistochemistry: CNOT2 Antibody (OTI5A12) [NBP2-46323]
Immunohistochemistry: CNOT2 Antibody (5A12) [NBP2-46323] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: CNOT2 Antibody (OTI5A12) [NBP2-46323]](https://resources.rndsystems.com/images/products/CNOT2-Antibody-5A12-Immunohistochemistry-NBP2-46323-img0002.jpg "Immunohistochemistry: CNOT2 Antibody (OTI5A12) [NBP2-46323]")
Immunohistochemistry: CNOT2 Antibody (OTI5A12) [NBP2-46323]
Immunohistochemistry: CNOT2 Antibody (5A12) [NBP2-46323] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: CNOT2 Antibody (OTI5A12) [NBP2-46323]](https://resources.rndsystems.com/images/products/CNOT2-Antibody-5A12-Immunohistochemistry-NBP2-46323-img0003.jpg "Immunohistochemistry: CNOT2 Antibody (OTI5A12) [NBP2-46323]")
Immunohistochemistry: CNOT2 Antibody (OTI5A12) [NBP2-46323]
Immunohistochemistry: CNOT2 Antibody (5A12) [NBP2-46323] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)Applications for CNOT2 Antibody (OTI5A12)
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CNOT2
Alternate Names
CCR4-associated factor 2, CCR4-NOT transcription complex, subunit 2, CDC36FLJ26456, negative regulator of transcription 2, NOT2CCR4-NOT transcription complex subunit 2, NOT2H
Gene Symbol
CNOT2
Additional CNOT2 Products
Product Documents for CNOT2 Antibody (OTI5A12)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CNOT2 Antibody (OTI5A12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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