CNOT8 Antibody (HL2586)
Novus Biologicals | Catalog # NBP3-48703
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2586
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Product Specifications
Immunogen
Recombinant fragment of human CNOT8.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CNOT8 Antibody (HL2586)
Western Blot: CNOT8 Antibody (HL2586) [NBP3-48703] -
Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: CNOT8 Antibody (HL2586) [NBP3-48703] -
CNOT8 antibody [HL2586] detects CNOT8 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CNOT8 stained by CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Western Blot: CNOT8 Antibody (HL2586) [NBP3-48703] -
Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: CNOT8 Antibody (HL2586) [NBP3-48703] -
Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: CNOT8 Antibody (HL2586) [NBP3-48703] -
Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: CNOT8 Antibody (HL2586) [NBP3-48703] -
Whole Japanese medaka extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for CNOT8 Antibody (HL2586)
Application
Recommended Usage
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CNOT8
Alternate Names
CAF1, CAF2, CALIFCAF1-like protein, CALIFp, CCR4-NOT transcription complex subunit 8, CCR4-NOT transcription complex, subunit 8, hCAF1, PGK promoter directed over production, POP2CCR4-associated factor 8
Gene Symbol
CNOT8
Additional CNOT8 Products
Product Documents for CNOT8 Antibody (HL2586)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CNOT8 Antibody (HL2586)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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