Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TF9-10H10

Format

BSA Free
View all Coagulation Factor III/Tissue Factor Primary Antibodies »

Product Specifications

Immunogen

Denatured Tissue factor isolated from human brain by the Factor VII affinity method

Reactivity Notes

Predicted cross-reactivities: Primate

Localization

Membrane; single pass type I membrane protein.

Specificity

CD142, also known as Tissue Factor, is the membrane receptor for coagulation factors VII and VIIa and is the cell surface initiator of coagulation. It is the major molecule of this type and is criticial for controlling hemostasis, thrombosis and inflammation. NB100-65221 is specific for an epitope within the extracellular domain, epitope locus I. It recognizes both the reduced and native non-reduced human and primate tissue factors. It does not inhibit coagulation or neutralize factor VII binding to CD142.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Coagulation Factor III/Tissue Factor Antibody (TF9-10H10) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1-10 ug/ml

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Coagulation Factor III/Tissue Factor

A series of complicated reactions take place in the body whenever a blood clot begins to form. The major initiator of these reactions is an enzyme system called the tissue factor/factor VIIa (TF/FVIIa) complex. Animal tests show that various inhibitors of the TF/FVIIa complex can minimize blood clot formation as well as inflammatory responses.

Alternate Names

CD142, F3, Thromboplastin, Tissue Factor

Entrez Gene IDs

2152 (Human)

Gene Symbol

F3

UniProt

P13726

Additional Coagulation Factor III/Tissue Factor Products

Product Documents for Coagulation Factor III/Tissue Factor Antibody (TF9-10H10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Coagulation Factor III/Tissue Factor Antibody (TF9-10H10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Coagulation Factor III/Tissue Factor Antibody (TF9-10H10) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Coagulation Factor III/Tissue Factor Antibody (TF9-10H10) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Could you please tell me the molecular weight of Tissue Factor? I want to use Antibody NB100-65221 for western blot.

    A: Tissue factor, also called Coagulation Factor III, is a 295 amino acids long protein and its molecular weight is approximately 33kDa.

  • Q: Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.

    A:

    Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

  • Q: Could you please tell me the molecular weight of Tissue Factor? I want to use Antibody NB100-65221 for western blot.

    A: Tissue factor, also called Coagulation Factor III, is a 295 amino acids long protein and its molecular weight is approximately 33kDa.

  • Q: Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.

    A:

    Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies

Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail