Coagulation Factor III/Tissue Factor Antibody

Novus Biologicals | Catalog # NBP2-15139

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Coagulation Factor III/Tissue Factor Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Coagulation Factor III/Tissue Factor. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Coagulation Factor III/Tissue Factor Antibody

Western Blot: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139]

Western Blot: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139]

Western Blot: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Coagulation factor III/Tissue Factor antibody [N1C3]diluted at 1:1000.
Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139] - Human endometrial carcinoma. Coagulation factor III/Tissue Factor stained by Coagulation factor III/Tissue Factor antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139] - Tissue Factor expression in paraffin-embedded human lung cancer tissues using Tissue Factor antibody. Image from verified customer review.

Applications for Coagulation Factor III/Tissue Factor Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:500 - 1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-15139 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Coagulation Factor III/Tissue Factor

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Alternate Names

CD142, F3, Thromboplastin, Tissue Factor

Gene Symbol

F3

UniProt

P13726

Additional Coagulation Factor III/Tissue Factor Products

Product Documents for Coagulation Factor III/Tissue Factor Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Coagulation Factor III/Tissue Factor Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Coagulation Factor III/Tissue Factor Antibody

Customer Reviews for Coagulation Factor III/Tissue Factor Antibody (1)

4 out of 5
1 Customer Rating
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Customer Images

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  • Name: Christopher England
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human lung cancer tissue samples
    Species: Human
    Verified Customer | Posted 09/03/2016
    Tissue Factor Expression in Lung Cancer Patient Samples
    Coagulation Factor III/Tissue Factor Antibody NBP2-15139

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Coagulation Factor III/Tissue Factor Antibody

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  • Q: Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.

    A: Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail