COASY Antibody (1H6) - Azide and BSA Free

Novus Biologicals | Catalog # H00080347-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1H6

Format

Azide and BSA Free
View all COASY Primary Antibodies »

Product Specifications

Immunogen

COASY (AAH06354, 1 a.a. ~ 225 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKGLGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRVFGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD

Reactivity Notes

Human. Other species not tested.

Specificity

COASY - Coenzyme A synthase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for COASY Antibody (1H6) - Azide and BSA Free

Western Blot: COASY Antibody (1H6) [H00080347-M01]

Western Blot: COASY Antibody (1H6) [H00080347-M01]

Western Blot: COASY Antibody (1H6) [H00080347-M01] - COASY monoclonal antibody (M01), clone 1H6 Analysis of COASY expression in A-431.
Western Blot: COASY Antibody (1H6) [H00080347-M01]

Western Blot: COASY Antibody (1H6) [H00080347-M01]

Western Blot: COASY Antibody (1H6) [H00080347-M01] - Analysis of COASY over-expressed 293 cell line, cotransfected with COASY Validated Chimera RNAi ( Cat # H00080347-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with COASY monoclonal antibody (M01), clone 1H6 (Cat # H00080347-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: COASY Antibody (1H6) [H00080347-M01]

Western Blot: COASY Antibody (1H6) [H00080347-M01]

Western Blot: COASY Antibody (1H6) [H00080347-M01] - Analysis of COASY expression in transfected 293T cell line by COASY monoclonal antibody (M01), clone 1H6.Lane 1: COASY transfected lysate(30.2 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: COASY Antibody (1H6) [H00080347-M01] - Detection limit for recombinant GST tagged COASY is approximately 0.1ng/ml as a capture antibody.

Applications for COASY Antibody (1H6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COASY

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Alternate Names

bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase, CoA synthaseUKR1, CoASY, Coenzyme A synthase, DPCK, FLJ35179, NBPbifunctional coenzyme A synthase, nucleotide binding protein, phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase, POV-2, PPAT

Entrez Gene IDs

80347 (Human)

Gene Symbol

COASY

UniProt

AAH06354 (Human)

Additional COASY Products

Product Documents for COASY Antibody (1H6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for COASY Antibody (1H6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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