Collagen V Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-26551

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Bovine, Equine

Cited:

Human

Applications

Validated:

Immunohistochemistry-Frozen, ELISA

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

Goats were hyperimmunized with human type V collagen.

Specificity

Reacts with conformational determinants on human type V collagen as demonstrated by ELISA. May react with type V collagen from other species. Exhibits <10% cross reactivity with collagen type I, II, III, IV and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Collagen V Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Collagen V Antibody [NBP1-26551]

Immunohistochemistry-Paraffin: Collagen V Antibody [NBP1-26551]

Immunohistochemistry-Paraffin: Collagen V Antibody [NBP1-26551] - Human gastric cancer tissue was stained with Goat Anti-Type V Collagen-UNLB followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP.
Immunohistochemistry-Frozen: Collagen V Antibody [NBP1-26551]

Immunohistochemistry-Frozen: Collagen V Antibody [NBP1-26551]

Immunohistochemistry-Frozen: Collagen V Antibody [NBP1-26551] - Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AF555 and DAPI.
Immunohistochemistry-Frozen: Collagen V Antibody [NBP1-26551]

Immunohistochemistry-Frozen: Collagen V Antibody [NBP1-26551]

Immunohistochemistry-Frozen: Collagen V Antibody [NBP1-26551] - Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB followed by Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF555 and DAPI.

Applications for Collagen V Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:4000

Immunohistochemistry-Frozen

1:20-1:40

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.4 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Collagen V

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.

Alternate Names

alpha 1 type V collagen, Collagen 5, collagen alpha-1(V) chain, collagen, type V, alpha 1, Collagen-5, EC 2.7.7.6, EC 6.1.1

Gene Symbol

COL5A1

Additional Collagen V Products

Product Documents for Collagen V Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Collagen V Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Collagen V Antibody - Azide and BSA Free

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Protocols

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