Collagen VI alpha 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-91195

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Collagen VI alpha 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VSVGIKDVFDFIPGSDQLNVISCQGLAPSQGRPGLSLVKENYAELLEDAFLKNVTAQICIDKKCPDYTCPITFSSPADITILLD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Collagen VI alpha 1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91195]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91195]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91195] - Collagen VI Antibody [NBP1-91195] - Staining in human gallbladder and pancreas tissues using anti-COL6A1 antibody. Corresponding COL6A1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Collagen VI alpha 1 Antibody [NBP1-91195]

Immunocytochemistry/ Immunofluorescence: Collagen VI alpha 1 Antibody [NBP1-91195]

Immunocytochemistry/Immunofluorescence: Collagen VI Antibody [NBP1-91195] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91195]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91195]

Immunohistochemistry-Paraffin: Collagen VI Antibody [NBP1-91195] - Staining of human gallbladder shows high expression.
Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91195]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91195]

Immunohistochemistry-Paraffin: Collagen VI Antibody [NBP1-91195] - Staining of human pancreas shows low expression as expected.
Collagen VI alpha 1 Antibody - BSA Free Immunohistochemistry: Collagen VI alpha 1 Antibody - BSA Free [NBP1-91195]

Immunohistochemistry: Collagen VI alpha 1 Antibody - BSA Free [NBP1-91195]

Analysis in human prostate and tonsil tissues using NBP1-91195 antibody. Corresponding COL6A1 RNA-seq data are presented for the same tissues.
Collagen VI alpha 1 Antibody - BSA Free Immunohistochemistry: Collagen VI alpha 1 Antibody - BSA Free [NBP1-91195]

Immunohistochemistry: Collagen VI alpha 1 Antibody - BSA Free [NBP1-91195]

Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Collagen VI alpha 1 Antibody - BSA Free Immunohistochemistry: Collagen VI alpha 1 Antibody - BSA Free [NBP1-91195]

Immunohistochemistry: Collagen VI alpha 1 Antibody - BSA Free [NBP1-91195]

Staining of human prostate shows strong membranous positivity in smooth muscle cells.
Collagen VI alpha 1 Antibody - BSA Free Immunohistochemistry: Collagen VI alpha 1 Antibody - BSA Free [NBP1-91195]

Immunohistochemistry: Collagen VI alpha 1 Antibody - BSA Free [NBP1-91195]

Staining of human testis shows strong membranous positivity in collagen fibers.
Collagen VI alpha 1 Antibody - BSA Free Immunohistochemistry: Collagen VI alpha 1 Antibody - BSA Free [NBP1-91195]

Immunohistochemistry: Collagen VI alpha 1 Antibody - BSA Free [NBP1-91195]

Staining of human duodenum shows strong membranous positivity in smooth muscle cells.

Applications for Collagen VI alpha 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Collagen VI alpha 1

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.

Alternate Names

Collagen 6, collagen alpha-1(VI) chain, collagen VI, alpha-1 polypeptide, collagen, type VI, alpha 1, Collagen-6, human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA), OPLL

Gene Symbol

COL6A1

Additional Collagen VI alpha 1 Products

Product Documents for Collagen VI alpha 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Collagen VI alpha 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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