Collagen VI alpha 3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-34837

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Collagen VI alpha 3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PRDLKIVVLMLTGEVPEQQLEEAQRVILQAKCKGYFFVVLGIGRKVNIKEVYTFASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSENAFYLSPDIRKQCDWFQGDQPTKNLVKFGHKQVNVPNNVTSSPTSNPVTTTKPVT

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Collagen VI alpha 3 Antibody - BSA Free

Western Blot: Collagen VI alpha 3 Antibody [NBP2-34837]

Western Blot: Collagen VI alpha 3 Antibody [NBP2-34837]

Western Blot: Collagen VI alpha 3 Antibody [NBP2-34837] - Analysis in human cell line U-87 MG.
Immunohistochemistry-Paraffin: Collagen VI alpha 3 Antibody [NBP2-34837]

Immunohistochemistry-Paraffin: Collagen VI alpha 3 Antibody [NBP2-34837]

Immunohistochemistry-Paraffin: Collagen VI alpha 3 Antibody [NBP2-34837] - Staining of human placenta shows moderate cytoplasmic positivity in connective tissues.
Immunohistochemistry-Paraffin: Collagen VI alpha 3 Antibody [NBP2-34837]

Immunohistochemistry-Paraffin: Collagen VI alpha 3 Antibody [NBP2-34837]

Immunohistochemistry-Paraffin: Collagen VI alpha 3 Antibody [NBP2-34837] - Staining of human ovary shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Collagen VI alpha 3 Antibody [NBP2-34837]

Immunohistochemistry-Paraffin: Collagen VI alpha 3 Antibody [NBP2-34837]

Immunohistochemistry-Paraffin: Collagen VI alpha 3 Antibody [NBP2-34837] - Staining of human cerebral cortex shows no positivity in neurons.
Simple Western: Collagen VI alpha 3 Antibody [NBP2-34837]

Simple Western: Collagen VI alpha 3 Antibody [NBP2-34837]

Simple Western: Collagen VI alpha 3 Antibody [NBP2-34837] - Lane view shows a specific band for Collagen IV alpha3 in 0.2 mg/ml of WI-38 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: Collagen VI alpha 3 Antibody [NBP2-34837]

Simple Western: Collagen VI alpha 3 Antibody [NBP2-34837]

Simple Western: Collagen VI alpha 3 Antibody [NBP2-34837] - Electropherogram image of the corresponding Simple Western lane view. Collagen IV alpha3 antibody was used at 1:25 dilution on WI-38 lysate(s) respectively.

Applications for Collagen VI alpha 3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Simple Western

1:25

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
See Simple Western Antibody Database for Simple Western validation: Tested in WI-38 lysates, separated by Size, antibody dilution of 1:25

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Collagen VI alpha 3

Alternate Names

Collagen Alpha-3(VI) Chain, Collagen VI, Alpha-3 Polypeptide, collagen, type VI, alpha 3

Gene Symbol

COL6A3

Additional Collagen VI alpha 3 Products

Product Documents for Collagen VI alpha 3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Collagen VI alpha 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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