Collagen XI alpha 2 Antibody (473) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43728

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (93%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 473

Format

Azide and BSA Free
View all Collagen XI alpha 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Collagen XI alpha 2. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

172 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Collagen XI alpha 2 Antibody (473) - Azide and BSA Free

Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728]

Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728]

Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human COL11A2-transfected 293T cells (N-terminal fragment of COL11A2 isoform 1) 12 % SDS-PAGE COL11A2 antibody [473] dilution: 1:5000
Collagen XI alpha 2 Antibody (473) - Azide and BSA Free

Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728] -

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL11A2 antibody [GT473] (NBP2-43728) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Collagen XI alpha 2 Antibody (473) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Collagen XI alpha 2 Antibody (473) [NBP2-43728] -

COL11A2 antibody [GT473] detects COL11A2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL11A2 protein stained by COL11A2 antibody [GT473] (NBP2-43728) diluted at 1:500.
Blue: Hoechst 33342 staining.
Collagen XI alpha 2 Antibody (473) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Collagen XI alpha 2 Antibody (473) [NBP2-43728] -

COL11A2 antibody [GT473] detects COL11A2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: COL11A2 protein stained by COL11A2 antibody [GT473] (NBP2-43728) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for Collagen XI alpha 2 Antibody (473) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Collagen XI alpha 2

Alternate Names

collagen alpha-2(XI) chain, collagen, type XI, alpha 2, DFNA13, DFNB53, HKE5, PARP, pro-a2 chain of collagen type XI, STL3

Gene Symbol

COL11A2

Additional Collagen XI alpha 2 Products

Product Documents for Collagen XI alpha 2 Antibody (473) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Collagen XI alpha 2 Antibody (473) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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