Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Collagen XXV alpha 1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide mapping to residues 430-443 [DYNGNLHEALQRIT] of the NC3 region of human and mouse CLAC-P.

Reactivity Notes

This antibody works in human and is expected to react with mouse protein.

Specificity

This detects full-length CLAC-P and the secreted form of CLAC-P protein. It does not react with full-length or secreted forms of COLXIII protein or the COLXXIII protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Collagen XXV alpha 1 Antibody - BSA Free

Western Blot: Collagen XXV alpha 1 AntibodyBSA Free [NB300-289]

Western Blot: Collagen XXV alpha 1 AntibodyBSA Free [NB300-289]

Western Blot: Collagen XXV alpha 1 Antibody [NB300-289] - Stable HEK293 cell line expressing CLAC-P using NB300-289. Lane 1: full-length CLAC-P (lysate). Lane 2: secreted form of CLAC-P (media). Lane 3: COLXIII (lysate). Lane 4 degrees COLXIII (media). Lane 5: COLXXIII (lysate). Lane 6: COLXXIII (media).
Immunohistochemistry: Collagen XXV alpha 1 Antibody - BSA Free [NB300-289]

Immunohistochemistry: Collagen XXV alpha 1 Antibody - BSA Free [NB300-289]

Immunohistochemistry: Collagen XXV Antibody [NB300-289] - Immunostain of the neocortex of Alzheimer's diseased brain sections (floating, fixed with 10% formalin for 24 hrs) using NB 300-289. At high magnification, NB 300-289 stains senile plaques.

Applications for Collagen XXV alpha 1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This antibody has been tested in Western blot and immunohistochemistry-Paraffin. Suggested Starting Dilutions:* Western Blot: 0.2 ug/ml (ECL Overexpressed protein)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Collagen XXV alpha 1

Antibodies against senile plaque (SP) amyloid obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with approximately 50/100 kDa polypeptides in Alzheimer's disease (AD) brains. The 9D2 antigen was identified as CLAC (collagen-like Alzheimer amyloid plaque component), and its precursor as CLAC-P/collagen type XXV. Both secreted and membrane-tethered forms of CLAC-P/collagen type XXV specifically bound to fibrillized Abeta, implicating these proteins in beta-amyloidogenesis and neuronal degeneration in AD.

Alternate Names

COL25A1

Entrez Gene IDs

84570 (Human)

Gene Symbol

COL25A1

Additional Collagen XXV alpha 1 Products

Product Documents for Collagen XXV alpha 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Collagen XXV alpha 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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