Complement Factor MASP3 Antibody (38:12-3)

Novus Biologicals | Catalog # NBP3-11301

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoassay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 38:12-3
View all Complement Factor MASP3 Primary Antibodies »

Product Specifications

Immunogen

The monoclonal antibody 38:12-3 recognizes MASP-3.

Specificity

The monoclonal antibody 38:12-3 does not recognize MASP-1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Description

Novus Biologicals Rat Complement Factor MASP3 Antibody (38:12-3) (NBP3-11301) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Applications for Complement Factor MASP3 Antibody (38:12-3)

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Complement Factor MASP3

The monoclonal antibody 38:12-3 recognizes MASP-3, a 94 kDa mannan-binding lectin (MBL)-associated serine protease. Three pathways of complement activation have been reported: the antibody-dependent classical pathway, the antibody-independent alternative pathway and the lectin pathway. Activation of each pathway involves formation of serine protease complexes, which results in activation of the central complement component, C3. In the lectin pathway, mannose- binding-lectin (MBL)-associated serine proteases (MASP) form complexes with oligopolymeric lectin molecules, which are involved in pattern recognition. Upon binding of the recognition molecules to carbohydrates on the surface of micro-organisms, MASPs are converted to their active forms and initiate complement activation. Six members of the family of MASP-like serine proteases have been reported: MASP-1, MASP-2, MASP-3, Map19, C1r and C1s. These products show identical domain organization, even though the overall amino acid sequence identity is only approximately 40%. Each MASP-type forms homodimers and is individually associated with MBL and the ficolins in a Ca2+-dependent matter. The four MBL-associated proteins are generated from only two genes, the MASP-1/3 gene encodes MASP-1 and MASP-3, whereas the MASP-2/Map19 gene gives rise to MASP-2 and the non-enzymatic MASP-2 MAp19. MASP-1 associates preferentially with low oligomer MBL (termed MBL-I), whereas MASP-2 and MASP-3 associate mainly with larger MBL oligomers (MBL II and MBL III). MASP-3 is generated by alternative splicing of the MASP-1/3 gene. The MASP-3 transcription product is composed of an A chain, which is common to both MASP-1 and MASP-3, and a B chain, which is unique to MASP-3. The biological function of MASP-3 remains unclear. However, MASP-3 was found to downregulate the C4 and C2 cleaving activity of MASP-2. MASP-3 inhibits MASP-2 by a combination of competitive inhibition of MASP-2 association with MBL and inhibition of activation of the MBL-associated MASP-2. The monoclonal antibody 38:12-3 does not recognize MASP-1.

Long Name

Mannan-binding lectin-Associated Serine Protease 3

Alternate Names

MASP1

Gene Symbol

MASP1

Additional Complement Factor MASP3 Products

Product Documents for Complement Factor MASP3 Antibody (38:12-3)

Certificate of Analysis

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Product Specific Notices for Complement Factor MASP3 Antibody (38:12-3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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