COMT Antibody

Novus Biologicals | Catalog # NB100-1780

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Flow Cytometry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all COMT Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence GDTKEQRILNHVLQC corresponding to N-Terminus according to NP_000745.1, NP_001128633.1, NP_001128634.1, NP_009294.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 19770404).

Specificity

This antibody is expected to recognize both reported isoforms. Variants (NP_000745.1; NP_001128633.1; NP_001128634.1) encode the same isoform.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat COMT Antibody (NB100-1780) is a polyclonal antibody validated for use in WB, ELISA and Flow. Anti-COMT Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for COMT Antibody

Western Blot: COMT Antibody [NB100-1780]

Western Blot: COMT Antibody [NB100-1780]

Western Blot: COMT Antibody [NB100-1780] - Staining of MCF7 (A) with antibody at 0.5 ug/mL, U251 cell lysate 1 (B) and U251 cell lysate 2 (C) (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence.
Flow Cytometry: COMT Antibody [NB100-1780]

Flow Cytometry: COMT Antibody [NB100-1780]

Flow Cytometry: COMT Antibody [NB100-1780] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: COMT Antibody [NB100-1780]

Western Blot: COMT Antibody [NB100-1780]

Western Blot: COMT Antibody [NB100-1780] - Staining of Human Liver (A) with antibody at 2 ug/mL and Ovary (B) lysate (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence.

Applications for COMT Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Peptide ELISA

Detection limit 1:4000

Western Blot

0.3 - 2 ug/mL

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COMT

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. The transcript variants are formed through the use of alternative translation initiation sites and promoters.

Long Name

Catechol O-Methyltransferase

Alternate Names

catechol O-methyltransferase, catechol-O-methyltransferase, EC 2.1.1.6

Entrez Gene IDs

1312 (Human)

Gene Symbol

COMT

UniProt

NP_000745.1 (Human)

Additional COMT Products

Product Documents for COMT Antibody

Certificate of Analysis

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Product Specific Notices for COMT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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