Connexin 50/GJA8 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-15748

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Connexin 50/GJA8 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 234-433 of human Connexin 50/GJA8 (NP_005258.2). SALKRPVEQPLGEIPEKSLHSIAVSSIQKAKGYQLLEEEKIVSHYFPLTEVGMVETSPLPAKPFNQFEEKISTGPLGDLSRGYQETLPSYAQVGAQEVEGEGPPAEEGAEPEVGEKKEEAERLTTEEQEKVAVPEGEKVETPGVDKEGEKEEPQSEKVSKQGLPAEKTPSLCPELTTDDARPLSRLSKASSRARSDDLTV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Connexin 50/GJA8 Antibody - Azide and BSA Free

Western Blot: Connexin 50/GJA8 AntibodyAzide and BSA Free [NBP3-15748]

Western Blot: Connexin 50/GJA8 AntibodyAzide and BSA Free [NBP3-15748]

Western Blot: Connexin 50/GJA8 Antibody [NBP3-15748] - Western blot analysis of extracts of Raji cells, using Connexin 50/GJA8 antibody (NBP3-15748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Connexin 50/GJA8 Antibody - Azide and BSA Free [NBP3-15748]

Immunocytochemistry/ Immunofluorescence: Connexin 50/GJA8 Antibody - Azide and BSA Free [NBP3-15748]

Immunocytochemistry/Immunofluorescence: Connexin 50/GJA8 Antibody [NBP3-15748] - Immunofluorescence analysis of L929 cells using Connexin 50/GJA8 antibody (NBP3-15748) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Connexin 50/GJA8 Antibody - Azide and BSA Free [NBP3-15748]

Immunocytochemistry/ Immunofluorescence: Connexin 50/GJA8 Antibody - Azide and BSA Free [NBP3-15748]

Immunocytochemistry/Immunofluorescence: Connexin 50/GJA8 Antibody [NBP3-15748] - Immunofluorescence analysis of C6 cells using Connexin 50/GJA8 antibody (NBP3-15748) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for Connexin 50/GJA8 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Connexin 50/GJA8

The 433 amino acid long, 48 kDA transmembrane connexin, gap junction alpha-8 protein encoded by the GJA8 gene is critical in growth and development of lens fiber cells. Mutations in the GJA8 gene may lead to zonular pulverulent cataracts, nuclear progressive cataracts, and cataract-microcornea syndrome. The GJA8 gene has been linked to myopia, microphthalmia, neuronitis, cycstic fibrosis, neuopathy, cervical adenocarcinoma, and juvenile myoclonic epilepsy. The GJA8 gene interacts with TJP1, MIP, MED14, GJA1, and GJB1 to participate in pathways such as gap junction assembly, membrane trafficking, and connexin synthesis.

Alternate Names

CAE1, CX50, CZP1, GJA8, MP70

Gene Symbol

GJA8

Additional Connexin 50/GJA8 Products

Product Documents for Connexin 50/GJA8 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Connexin 50/GJA8 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Ion Channels and Regulators

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Protocols

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