Coronin-1a Antibody (OTI1A5)

Novus Biologicals | Catalog # NBP2-02779

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A5
View all Coronin-1a Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CORO1A(NP_009005) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Coronin-1a Antibody (OTI1A5)

Western Blot: Coronin-1a Antibody (1A5) [NBP2-02779] Analysis of extracts (35ug) from 9 different cell lines by using anti-Coronin-1a monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Coronin-1a Antibody (OTI1A5) [NBP2-02779]

Immunocytochemistry/ Immunofluorescence: Coronin-1a Antibody (OTI1A5) [NBP2-02779]

Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody (1A5) [NBP2-02779] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Coronin-1a.
Flow Cytometry: Coronin-1a Antibody (OTI1A5) [NBP2-02779]

Flow Cytometry: Coronin-1a Antibody (OTI1A5) [NBP2-02779]

Flow Cytometry: Coronin-1a Antibody (1A5) [NBP2-02779] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Coronin-1a antibody, and then analyzed by flow cytometry.
Western Blot: Coronin-1a Antibody (OTI1A5) [NBP2-02779]

Western Blot: Coronin-1a Antibody (OTI1A5) [NBP2-02779]

Western Blot: Coronin-1a Antibody (1A5) [NBP2-02779] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Coronin-1a (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Coronin-1a.
Flow Cytometry: Coronin-1a Antibody (OTI1A5) [NBP2-02779]

Flow Cytometry: Coronin-1a Antibody (OTI1A5) [NBP2-02779]

Flow Cytometry: Coronin-1a Antibody (1A5) [NBP2-02779] - Analysis of Hela cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Coronin-1a Antibody (OTI1A5) [NBP2-02779]

Flow Cytometry: Coronin-1a Antibody (OTI1A5) [NBP2-02779]

Flow Cytometry: Coronin-1a Antibody (1A5) [NBP2-02779] - Analysis of Jurkat cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for Coronin-1a Antibody (OTI1A5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Coronin-1a

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq].

Alternate Names

CLIPINA, Clipin-A, CORO1, coronin, actin binding protein, 1A, coronin, actin-binding protein, 1A, coronin-1, coronin-1A, Coronin-like protein A, Coronin-like protein p57, FLJ41407, HCORO1, MGC117380, p57, TACOCLABP, Tryptophan aspartate-containing coat protein

Entrez Gene IDs

11151 (Human)

Gene Symbol

CORO1A

Additional Coronin-1a Products

Product Documents for Coronin-1a Antibody (OTI1A5)

Certificate of Analysis

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Product Specific Notices for Coronin-1a Antibody (OTI1A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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