Cotton Rat CD8 alpha alpha Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7080G
Detection of CD8 alpha  in Cotton Rat Splenocytes by Flow Cytometry.
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Cotton Rat CD8 alpha alpha Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Cotton Rat
Specificity
Detects cotton rat CD8 alpha in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # JG12
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NIH‑3T3 mouse embryonic fibroblast cell line transfected with cotton rat CD8 alpha and SP2/O mouse melanoma cell line transfected with cotton rat CD8 alpha.
Accession # AAL55392
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CD8a antibody in Cotton Rat Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD8 alpha in Cotton Rat Splenocytes by Flow Cytometry. Cotton rat splenocytes were stained with Mouse Anti-Cotton Rat CD8a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7080G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of CD8 alpha in NS0 Mouse Cell Line Transfected with Cotton Rat CD8 alpha by Flow Cytometry. NS0 mouse myeloma cell line transfected with cotton rat CD8a were stained with Mouse Anti-Cotton Rat CD8a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7080G, filled histogram) or Mouse IgG2A isotype control antibody (IC003G, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD8

CD8 alpha (CD8 alpha ; also OX8, Leu-2 and Lyt-2) is a 23 kDa (predicted) member of the Ig superfamily of molecules. It is found on immature thymocytes, cytotoxic and suppressor T cells, select mast cells, and splenic and bone marrow dendritic cells (in rodent). On T cells, CD8 alpha exists as either a disulfide linked homodimer, or heterodimer bound to CD8 beta. CD8 alpha beta is best known as a coreceptor for the TCR, enhancing TCR signaling. CD8 alpha alpha serves a different function and acts as a TCR corepressor that blocks T cell activation. Based on rat, mature Sigmodon hispidus/cotton rat CD8 alpha is a 210 amino acid (aa) type I transmembrane protein that consists of a 161 aa extracellular region (aa 24-184) with one V-type Ig-like domain (aa 35-135), and a 30 aa cytoplasmic tail (aa 206-235). Although Sigmodon hispidus is called a rat, it is not. It is a rodent, and rat CD8 alpha is the closest ortholog to cotton rat CD8 alpha currently reported. Over the extracellular region, rat and cotton rat CD8 alpha share only 54% aa identity. Rat CD8 alpha runs at 34-39 kDa in SDS-Page, and possesses one utilized N-linked and O-linked glycosylation site (vs. no N-linked sites in cotton rat CD8 alpha ). Based on this organization, and assuming O-linked glycosylation, cotton rat CD8 alpha would be expected to run at about 30-34 kDa in SDS-PAGE. Over aa 24-182, cotton rat CD8 alpha shares 54% and 48% aa identity with rat and mouse CD8 alpha, respectively.   

Entrez Gene IDs
925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names
CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Cotton Rat CD8 alpha alpha Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Development of Acquired Immunity following Repeated Respiratory Syncytial Virus Infections in Cotton Rats
    PLoS ONE, 2016;11(5):e0155777.
    Species: Cotton Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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