Cotton Rat CD8 alpha Antibody

Catalog # Availability Size / Price Qty
MAB7080
MAB7080-SP
Cotton Rat CD8 alpha  Antibody in Data
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Cotton Rat CD8 alpha Antibody Summary

Species Reactivity
Cotton Rat
Specificity
Detects cotton rat CD8 alpha in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # JG12
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NIH‑3T3 mouse embryonic fibroblast cell line transfected with cotton rat CD8 alpha and SP2/O mouse melanoma cell line transfected with cotton rat CD8 alpha.
Accession # AAL55392
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD8 alpha  in Cotton Rat Splenocytes by Flow Cytometry. View Larger

Detection of CD8 alpha in Cotton Rat Splenocytes by Flow Cytometry. Cotton rat splenocytes were stained with Mouse Anti-Cotton Rat CD8a Monoclonal Antibody (Catalog # MAB7080, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8 alpha (CD8 alpha ; also OX8, Leu-2 and Lyt-2) is a 23 kDa (predicted) member of the Ig superfamily of molecules. It is found on immature thymocytes, cytotoxic and suppressor T cells, select mast cells, and splenic and bone marrow dendritic cells (in rodent). On T cells, CD8 alpha exists as either a disulfide linked homodimer, or heterodimer bound to CD8 beta. CD8 alpha beta is best known as a coreceptor for the TCR, enhancing TCR signaling. CD8 alpha alpha serves a different function and acts as a TCR corepressor that blocks T cell activation. Based on rat, mature Sigmodon hispidus/cotton rat CD8 alpha is a 210 amino acid (aa) type I transmembrane protein that consists of a 161 aa extracellular region (aa 24-184) with one V-type Ig-like domain (aa 35-135), and a 30 aa cytoplasmic tail (aa 206-235). Although Sigmodon hispidus is called a rat, it is not. It is a rodent, and rat CD8 alpha is the closest ortholog to cotton rat CD8 alpha currently reported. Over the extracellular region, rat and cotton rat CD8 alpha share only 54% aa identity. Rat CD8 alpha runs at 34-39 kDa in SDS-Page, and possesses one utilized N-linked and O-linked glycosylation site (vs. no N-linked sites in cotton rat CD8 alpha ). Based on this organization, and assuming O-linked glycosylation, cotton rat CD8 alpha would be expected to run at about 30-34 kDa in SDS-PAGE. Over aa 24-182, cotton rat CD8 alpha shares 54% and 48% aa identity with rat and mouse CD8 alpha, respectively.   

Entrez Gene IDs
925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names
CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Cotton Rat CD8 alpha Antibody

There are currently no reviews for this product. Be the first to review Cotton Rat CD8 alpha Antibody and earn rewards!

Have you used Cotton Rat CD8 alpha Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review