COX-1 Antibody (6B6) - BSA Free

Novus Biologicals | Catalog # NBP3-26362

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6B6 expressed in HEK293

Format

BSA Free
View all COX-1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human COX-1 [UniProt P23219]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COX-1 Antibody (6B6) - BSA Free

COX-1 Antibody (6B6)

Western Blot: COX-1 Antibody (6B6) [NBP3-26362] -

Western Blot: COX-1 Antibody (6B6) [NBP3-26362] - Positive Western Blot detected in: HL60 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissue
All lanes: COX-1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 69, 65, 62, 57, 72, 73 kDa
Observed band size: 72 kDa
COX-1 Antibody (6B6)

Flow Cytometry: COX-1 Antibody (6B6) [NBP3-26362] -

Flow Cytometry: COX-1 Antibody (6B6) [NBP3-26362] - Overlay histogram showing Hela cells stained with COX-1 Antibody (6B6) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for COX-1 Antibody (6B6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:20-1:200

Western Blot

1:500-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: COX-1

Cyclooxygenase (COX), also known as prostaglandin G/H synthase, is a membrane bound enzyme partly responsible for the catalysis of prostanoid synthesis. COX is expressed as at least three different isoforms. COX-1 is constitutively expressed and thought to regulate a number of 'housekeeping' functions such as vascular hemostasis, renal blood flow, and glomerular function. COX-2 expression is tightly regulated and induced by inflammatory mediators such as growth factors, cytokines, and endotoxin. COX-3 appears to be much more strictly regulated spatially and is observed in greatest abundance in cerebral cortex.

Long Name

Cyclooxygenase 1

Alternate Names

COX1, PTGS1

Gene Symbol

PTGS1

Additional COX-1 Products

Product Documents for COX-1 Antibody (6B6) - BSA Free

Certificate of Analysis

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Product Specific Notices for COX-1 Antibody (6B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for COX-1 Antibody (6B6) - BSA Free

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  • Q: Please could you tell me if Novus Biologicals has an active COX1 Recombinant Protein?

    A: Unfortunately we do not currently have any COX1 recombinant proteins that are expected to be active.

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