COX-2 Antibody (COX2/7803R) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-21139

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # COX2/7803R

Format

Azide and BSA Free
View all COX-2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa400-604) of human COX-2 protein (exact sequence is proprietary)

Localization

Cytoplasm. Cell surface.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Human colon or lung carcinoma tissue (IHC).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for COX-2 Antibody (COX2/7803R) - Azide and BSA Free

COX-2 Antibody (COX2/7803R) - Azide and BSA Free

COX-2 Antibody (COX2/7803R)

Formalin-fixed, paraffin-embedded human prostate stained with COX-2 antibody (COX2/7803R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
COX-2 Antibody (COX2/7803R) - Azide and BSA Free

COX-2 Antibody (COX2/7803R)

Formalin-fixed, paraffin-embedded human prostate stained with COX-2 antibody (COX2/7803R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
COX-2 Antibody (COX2/7803R) - Azide and BSA Free Western Blot: COX-2 Antibody (COX2/7803R) - Azide and BSA Free [NBP3-21139] -

Western Blot: COX-2 Antibody (COX2/7803R) - Azide and BSA Free [NBP3-21139] -

Western blot analysis of NIH/3T3 cell lysate using COX-2 Antibody (COX2/7803R) - Azide and BSA Free.

Applications for COX-2 Antibody (COX2/7803R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: COX-2

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Long Name

Cyclooxygenase 2

Alternate Names

COX2, PGHS-2, PHS-II, PTGS2

Gene Symbol

PTGS2

Additional COX-2 Products

Product Documents for COX-2 Antibody (COX2/7803R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for COX-2 Antibody (COX2/7803R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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